Amylase-Sensitive Polymeric Nanoparticles Based on Dextran Sulfate and Doxorubicin with Anticoagulant Activity
This study looked into the synthesis and study of Dextrane Sulfate−Doxorubicin Nanoparticles (DS−Dox NP) that are sensitive to amylase and show anticoagulant properties. The particles were obtained by the method of solvent replacement. They had a size of 305 ± 58 nm, wit...
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doaj-8d0a00e5084041d2ab322a4f6b7167542020-11-25T00:48:18ZengMDPI AGPolymers2073-43602019-05-0111592110.3390/polym11050921polym11050921Amylase-Sensitive Polymeric Nanoparticles Based on Dextran Sulfate and Doxorubicin with Anticoagulant ActivityNikolay A. Pyataev0Pavel S. Petrov1Olga V. Minaeva2Mikhail N. Zharkov3Oleg A. Kulikov4Axeksandr V. Kokorev5Ekaterina P. Brodovskaya6Ivan A. Yurlov7Ilya V. Syusin8Andrey V. Zaborovskiy9Larisa A. Balykova10National Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaNational Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaNational Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaNational Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaNational Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaNational Research Nuclear University MEPhI (IATE MEPHI), 31 Kashirskoe shosse, Moscow 115409, RussiaNational Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaNational Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaNational Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaMoscow State University of Medicine and Dentistry named after A.I. Evdokimov, 20/1 Delegatskaya Str., Moscow 127473, RussiaNational Research Ogarev Mordovia State University, 68 Bolshevistskaya Str., Saransk 430005, Republic of Mordovia, RussiaThis study looked into the synthesis and study of Dextrane Sulfate−Doxorubicin Nanoparticles (DS−Dox NP) that are sensitive to amylase and show anticoagulant properties. The particles were obtained by the method of solvent replacement. They had a size of 305 ± 58 nm, with a mass ratio of DS:Dox = 3.3:1. On heating to 37 °C, the release of Dox from the particles was equal to 24.2% of the drug contained. In the presence of amylase, this ratio had increased to 42.1%. The study of the biological activity of the particles included an assessment of the cytotoxicity and the effect on hemostasis and antitumor activity. In a study of cytotoxicity on the L929 cell culture, it was found that the synthesized particles had less toxicity, compared to free doxorubicin. However, in the presence of amylase, their cytotoxicity was higher than the traditional forms of the drug. In a study of the effect of DS−Dox NP on hemostasis, it was found that the particles had a heparin-like anticoagulant effect. Antitumor activity was studied on the model of ascitic Zaidel hepatoma in rats. The frequency of complete cure in animals treated with the DS−Dox nanoparticles was higher, compared to animals receiving the traditional form of the drug.https://www.mdpi.com/2073-4360/11/5/921doxorubicindextran sulfatenanoparticlesamylasecytotoxicityascitic hepatomaheparin-like effect |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nikolay A. Pyataev Pavel S. Petrov Olga V. Minaeva Mikhail N. Zharkov Oleg A. Kulikov Axeksandr V. Kokorev Ekaterina P. Brodovskaya Ivan A. Yurlov Ilya V. Syusin Andrey V. Zaborovskiy Larisa A. Balykova |
spellingShingle |
Nikolay A. Pyataev Pavel S. Petrov Olga V. Minaeva Mikhail N. Zharkov Oleg A. Kulikov Axeksandr V. Kokorev Ekaterina P. Brodovskaya Ivan A. Yurlov Ilya V. Syusin Andrey V. Zaborovskiy Larisa A. Balykova Amylase-Sensitive Polymeric Nanoparticles Based on Dextran Sulfate and Doxorubicin with Anticoagulant Activity Polymers doxorubicin dextran sulfate nanoparticles amylase cytotoxicity ascitic hepatoma heparin-like effect |
author_facet |
Nikolay A. Pyataev Pavel S. Petrov Olga V. Minaeva Mikhail N. Zharkov Oleg A. Kulikov Axeksandr V. Kokorev Ekaterina P. Brodovskaya Ivan A. Yurlov Ilya V. Syusin Andrey V. Zaborovskiy Larisa A. Balykova |
author_sort |
Nikolay A. Pyataev |
title |
Amylase-Sensitive Polymeric Nanoparticles Based on Dextran Sulfate and Doxorubicin with Anticoagulant Activity |
title_short |
Amylase-Sensitive Polymeric Nanoparticles Based on Dextran Sulfate and Doxorubicin with Anticoagulant Activity |
title_full |
Amylase-Sensitive Polymeric Nanoparticles Based on Dextran Sulfate and Doxorubicin with Anticoagulant Activity |
title_fullStr |
Amylase-Sensitive Polymeric Nanoparticles Based on Dextran Sulfate and Doxorubicin with Anticoagulant Activity |
title_full_unstemmed |
Amylase-Sensitive Polymeric Nanoparticles Based on Dextran Sulfate and Doxorubicin with Anticoagulant Activity |
title_sort |
amylase-sensitive polymeric nanoparticles based on dextran sulfate and doxorubicin with anticoagulant activity |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2019-05-01 |
description |
This study looked into the synthesis and study of Dextrane Sulfate−Doxorubicin Nanoparticles (DS−Dox NP) that are sensitive to amylase and show anticoagulant properties. The particles were obtained by the method of solvent replacement. They had a size of 305 ± 58 nm, with a mass ratio of DS:Dox = 3.3:1. On heating to 37 °C, the release of Dox from the particles was equal to 24.2% of the drug contained. In the presence of amylase, this ratio had increased to 42.1%. The study of the biological activity of the particles included an assessment of the cytotoxicity and the effect on hemostasis and antitumor activity. In a study of cytotoxicity on the L929 cell culture, it was found that the synthesized particles had less toxicity, compared to free doxorubicin. However, in the presence of amylase, their cytotoxicity was higher than the traditional forms of the drug. In a study of the effect of DS−Dox NP on hemostasis, it was found that the particles had a heparin-like anticoagulant effect. Antitumor activity was studied on the model of ascitic Zaidel hepatoma in rats. The frequency of complete cure in animals treated with the DS−Dox nanoparticles was higher, compared to animals receiving the traditional form of the drug. |
topic |
doxorubicin dextran sulfate nanoparticles amylase cytotoxicity ascitic hepatoma heparin-like effect |
url |
https://www.mdpi.com/2073-4360/11/5/921 |
work_keys_str_mv |
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