Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO-1 signaling in RAW 264.7 cells
Abstract Background Among the different kinds of pollution, air pollution continues to increase globally. East Asia is considered to be significantly affected. As a result, the populations in these regions face serious health issues including respiratory disorders. This study investigated the impact...
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doaj-8d10f75aa3d64462ae8e959a35b67fb62020-11-25T04:01:41ZengBMCBMC Complementary and Alternative Medicine1472-68822018-09-0118111010.1186/s12906-018-2314-6Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO-1 signaling in RAW 264.7 cellsThilina U. Jayawardena0K. K. Asanka Sanjeewa1I. P. Shanura Fernando2Bo Mi Ryu3Min-Cheol Kang4Youngheun Jee5Won Woo Lee6You-Jin Jeon7Department of Marine Life Sciences, Jeju National UniversityDepartment of Marine Life Sciences, Jeju National UniversityDepartment of Marine Life Sciences, Jeju National UniversityDepartment of Marine Life Sciences, Jeju National UniversityDepartment of Marine Life Sciences, Jeju National UniversityDepartment of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National UniversityDepartment of Marine Life Sciences, Jeju National UniversityDepartment of Marine Life Sciences, Jeju National UniversityAbstract Background Among the different kinds of pollution, air pollution continues to increase globally. East Asia is considered to be significantly affected. As a result, the populations in these regions face serious health issues including respiratory disorders. This study investigated the impact of fine dust (FD) particles (CRM No. 28) on macrophage cells as a model for alveolar lung cells. Methods The research focused on inflammation and oxidative stress induced by FD and Sargassum horneri (Turner) C. Agardh ethanol extract (SHE) as a potential treatment. S. horneri is a type of brown algae that has demonstrated anti-inflammatory effects against RAW 264.7 macrophages in previous studies. MTT, Griess, ELISA, western blotting, and mRNA expression analyses using PCR techniques were used in this study. Results The optimum FD concentration was determined to be 125 μg mL− 1. FD particles stimulated inflammatory mediators production (iNOS, COX-2, and PGE2) and pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α), leading to NO production. These mediators were dose-dependently downregulated by treatment with SHE. IL-6 and TNF-α were identified as biomarkers for FD. SHE treatment induced HO-1 and Nrf2 activity in a dose-dependent manner under FD stimulation. This confirmed the cytoprotective effect against oxidative stress induced via FD. Furthermore, treatment of the cells with a p38 MAPK inhibitor (SB202190) induced FD-stimulated NO production. Conclusions The results suggest that SHE increases macrophage cellular resistance to FD-induced inflammation and oxidative stress, probably via the p38 MAPK pathway and Nrf2/HO-1 expression.http://link.springer.com/article/10.1186/s12906-018-2314-6Sargassum horneri (Turner) C. AgardhInflammationOxidative stressRAW 264.7 macrophagesNrf2/HO-1p38 MAPK |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thilina U. Jayawardena K. K. Asanka Sanjeewa I. P. Shanura Fernando Bo Mi Ryu Min-Cheol Kang Youngheun Jee Won Woo Lee You-Jin Jeon |
spellingShingle |
Thilina U. Jayawardena K. K. Asanka Sanjeewa I. P. Shanura Fernando Bo Mi Ryu Min-Cheol Kang Youngheun Jee Won Woo Lee You-Jin Jeon Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO-1 signaling in RAW 264.7 cells BMC Complementary and Alternative Medicine Sargassum horneri (Turner) C. Agardh Inflammation Oxidative stress RAW 264.7 macrophages Nrf2/HO-1 p38 MAPK |
author_facet |
Thilina U. Jayawardena K. K. Asanka Sanjeewa I. P. Shanura Fernando Bo Mi Ryu Min-Cheol Kang Youngheun Jee Won Woo Lee You-Jin Jeon |
author_sort |
Thilina U. Jayawardena |
title |
Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO-1 signaling in RAW 264.7 cells |
title_short |
Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO-1 signaling in RAW 264.7 cells |
title_full |
Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO-1 signaling in RAW 264.7 cells |
title_fullStr |
Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO-1 signaling in RAW 264.7 cells |
title_full_unstemmed |
Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2/HO-1 signaling in RAW 264.7 cells |
title_sort |
sargassum horneri (turner) c. agardh ethanol extract inhibits the fine dust inflammation response via activating nrf2/ho-1 signaling in raw 264.7 cells |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2018-09-01 |
description |
Abstract Background Among the different kinds of pollution, air pollution continues to increase globally. East Asia is considered to be significantly affected. As a result, the populations in these regions face serious health issues including respiratory disorders. This study investigated the impact of fine dust (FD) particles (CRM No. 28) on macrophage cells as a model for alveolar lung cells. Methods The research focused on inflammation and oxidative stress induced by FD and Sargassum horneri (Turner) C. Agardh ethanol extract (SHE) as a potential treatment. S. horneri is a type of brown algae that has demonstrated anti-inflammatory effects against RAW 264.7 macrophages in previous studies. MTT, Griess, ELISA, western blotting, and mRNA expression analyses using PCR techniques were used in this study. Results The optimum FD concentration was determined to be 125 μg mL− 1. FD particles stimulated inflammatory mediators production (iNOS, COX-2, and PGE2) and pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α), leading to NO production. These mediators were dose-dependently downregulated by treatment with SHE. IL-6 and TNF-α were identified as biomarkers for FD. SHE treatment induced HO-1 and Nrf2 activity in a dose-dependent manner under FD stimulation. This confirmed the cytoprotective effect against oxidative stress induced via FD. Furthermore, treatment of the cells with a p38 MAPK inhibitor (SB202190) induced FD-stimulated NO production. Conclusions The results suggest that SHE increases macrophage cellular resistance to FD-induced inflammation and oxidative stress, probably via the p38 MAPK pathway and Nrf2/HO-1 expression. |
topic |
Sargassum horneri (Turner) C. Agardh Inflammation Oxidative stress RAW 264.7 macrophages Nrf2/HO-1 p38 MAPK |
url |
http://link.springer.com/article/10.1186/s12906-018-2314-6 |
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