Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation
TIA-1 related protein (TIAR) is a RNA-binding protein involved in several steps of gene expression such as RNA splicing Aznarez et al. (2008) [1] and translation Piecyk et al. (2000) [2]. TIAR contains three RNA recognition motifs (RRMs) allowing its interaction with specific sequences localized in...
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doaj-8d1231930ac54387953d318755df42d62020-11-25T02:37:05ZengElsevierGenomics Data2213-59602016-03-017297300Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulationYacine Kharraz0Anne Lefort1Frédérick Libert2Christopher J. Mann3Cyril Gueydan4Véronique Kruys5Laboratoire de Biologie Moléculaire du Gène, Faculté des Sciences, BelgiumInstitut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Faculté de Médecine, Université Libre de Bruxelles (ULB), BelgiumInstitut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Faculté de Médecine, Université Libre de Bruxelles (ULB), BelgiumAsphalion S.L., 08006 Barcelona, SpainLaboratoire de Biologie Moléculaire du Gène, Faculté des Sciences, BelgiumLaboratoire de Biologie Moléculaire du Gène, Faculté des Sciences, BelgiumTIA-1 related protein (TIAR) is a RNA-binding protein involved in several steps of gene expression such as RNA splicing Aznarez et al. (2008) [1] and translation Piecyk et al. (2000) [2]. TIAR contains three RNA recognition motifs (RRMs) allowing its interaction with specific sequences localized in the untranslated regions (UTRs) of several mRNAs. In myeloid cells, TIAR has been shown to bind and regulate the translation and stability of various mRNA-encoding proteins important for the inflammatory response, such as TNFα Piecyk et al. (2000), Gueydan et al. (1999) [2,3], Cox-2 Cok et al. (2003) [4] or IL-8 Suswam et al. (2005) [5]. Here, we generated two macrophage-like RAW 264.7 cell lines expressing either a tagged full-length TIAR protein or a RRM2-truncated mutant unable to bind RNA with high affinity Dember et al. (1996), Kim et al. (2013) . By a combination of RNA-IP and microarray analysis (RIP-chip), we identified mRNAs specifically bound by the full-length protein both in basal conditions and in response to LPS (GSE77577). Keywords: RIP-chip, AU-rich element, TIAR, Translation repression, LPShttp://www.sciencedirect.com/science/article/pii/S2213596016300265 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yacine Kharraz Anne Lefort Frédérick Libert Christopher J. Mann Cyril Gueydan Véronique Kruys |
spellingShingle |
Yacine Kharraz Anne Lefort Frédérick Libert Christopher J. Mann Cyril Gueydan Véronique Kruys Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation Genomics Data |
author_facet |
Yacine Kharraz Anne Lefort Frédérick Libert Christopher J. Mann Cyril Gueydan Véronique Kruys |
author_sort |
Yacine Kharraz |
title |
Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation |
title_short |
Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation |
title_full |
Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation |
title_fullStr |
Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation |
title_full_unstemmed |
Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation |
title_sort |
genome-wide analysis of tiar rna ligands in mouse macrophages before and after lps stimulation |
publisher |
Elsevier |
series |
Genomics Data |
issn |
2213-5960 |
publishDate |
2016-03-01 |
description |
TIA-1 related protein (TIAR) is a RNA-binding protein involved in several steps of gene expression such as RNA splicing Aznarez et al. (2008) [1] and translation Piecyk et al. (2000) [2]. TIAR contains three RNA recognition motifs (RRMs) allowing its interaction with specific sequences localized in the untranslated regions (UTRs) of several mRNAs. In myeloid cells, TIAR has been shown to bind and regulate the translation and stability of various mRNA-encoding proteins important for the inflammatory response, such as TNFα Piecyk et al. (2000), Gueydan et al. (1999) [2,3], Cox-2 Cok et al. (2003) [4] or IL-8 Suswam et al. (2005) [5]. Here, we generated two macrophage-like RAW 264.7 cell lines expressing either a tagged full-length TIAR protein or a RRM2-truncated mutant unable to bind RNA with high affinity Dember et al. (1996), Kim et al. (2013) . By a combination of RNA-IP and microarray analysis (RIP-chip), we identified mRNAs specifically bound by the full-length protein both in basal conditions and in response to LPS (GSE77577). Keywords: RIP-chip, AU-rich element, TIAR, Translation repression, LPS |
url |
http://www.sciencedirect.com/science/article/pii/S2213596016300265 |
work_keys_str_mv |
AT yacinekharraz genomewideanalysisoftiarrnaligandsinmousemacrophagesbeforeandafterlpsstimulation AT annelefort genomewideanalysisoftiarrnaligandsinmousemacrophagesbeforeandafterlpsstimulation AT fredericklibert genomewideanalysisoftiarrnaligandsinmousemacrophagesbeforeandafterlpsstimulation AT christopherjmann genomewideanalysisoftiarrnaligandsinmousemacrophagesbeforeandafterlpsstimulation AT cyrilgueydan genomewideanalysisoftiarrnaligandsinmousemacrophagesbeforeandafterlpsstimulation AT veroniquekruys genomewideanalysisoftiarrnaligandsinmousemacrophagesbeforeandafterlpsstimulation |
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