Isolamento e avaliação da atividade citotóxica de alguns alcalóides oxaporfínicos obtidos de annonaceae Isolation and cytotoxicity evaluation of some oxoaporphine alkaloids from annonaceae

• Main Authors: Denise Brentan da Silva, Maria de Fátima Cepa Matos, Simone T. Nakashita, Carina K. Misu, Nídia Cristiane Yoshida, Carlos Alexandre Carollo, João Roberto Fabri, Hércules da Silva Miglio, João Máximo de Siqueira
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2007-01-01
• Series: Química Nova
• Subjects: oxoaporphine; chromatography; cytotoxicity
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000800004
• ISSN: 0100-4042; 1678-7064

<abstract language="eng">A different methodology was used to isolate and purify oxoaporphine alkaloids, as they are difficult to separate by the usual workup when in mixture. Alkaloid extracts from Annonaceae species were obtained by base/acid extraction. The extracts were concentrated and submitted to partition in solutions of acids of different pKa values, followed by separation by preparative TLC using 1 mm thick silica gel impregnated with oxalic acid (11.2% w/w). Liriodenine, lisycamine, lanuginosine, and O-methylmoschatoline were obtained and tested against tumoral cells (line Hep2, ATCC-CCL 23, larynx carcinoma). Only O-methylmoschatoline (IC50 12.4 µM) was more active than cisplatin (18.0 µM).