Comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytes
The integrin α6β4 and cytoskeletal adaptor plectin are essential components of type I and type II hemidesmosomes (HDs). We recently identified an alternative type II HD adhesion complex that also contains CD151 and the integrin α3β1. Here, we have taken a BioID proximity labeling approach to define...
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2020-08-01
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doaj-8d15bd97a4d342e3943ac82617d013292021-06-02T14:42:35ZengThe Company of BiologistsBiology Open2046-63902020-08-019810.1242/bio.054155054155Comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytesLisa te Molder0Liesbeth Hoekman1Maaike Kreft2Onno Bleijerveld3Arnoud Sonnenberg4 Division of Cell Biology I, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands Mass Spectrometry/Proteomics Facility, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands Division of Cell Biology I, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands Mass Spectrometry/Proteomics Facility, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands Division of Cell Biology I, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, The Netherlands The integrin α6β4 and cytoskeletal adaptor plectin are essential components of type I and type II hemidesmosomes (HDs). We recently identified an alternative type II HD adhesion complex that also contains CD151 and the integrin α3β1. Here, we have taken a BioID proximity labeling approach to define the proximity protein environment for α6β4 in keratinocytes. We identified 37 proteins that interacted with both α6 and β4, while 20 and 78 proteins specifically interacted with the α6 and β4 subunits, respectively. Many of the proximity interactors of α6β4 are components of focal adhesions (FAs) and the cortical microtubule stabilizing complex (CMSC). Though the close association of CMSCs with α6β4 in HDs was confirmed by immunofluorescence analysis, CMSCs have no role in the assembly of HDs. Analysis of the β4 interactome in the presence or absence of CD151 revealed that they are strikingly similar; only 11 different interactors were identified. One of these was the integrin α3β1, which interacted with α6β4 more strongly in the presence of CD151 than in its absence. These findings indicate that CD151 does not significantly contribute to the interactome of α6β4, but suggest a role of CD151 in linking α3β1 and α6β4 together in tetraspanin adhesion structures.http://bio.biologists.org/content/9/8/bio054155integrininteractomebioidhemidesmosomecortical microtubule stabilizing complextetraspanin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lisa te Molder Liesbeth Hoekman Maaike Kreft Onno Bleijerveld Arnoud Sonnenberg |
spellingShingle |
Lisa te Molder Liesbeth Hoekman Maaike Kreft Onno Bleijerveld Arnoud Sonnenberg Comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytes Biology Open integrin interactome bioid hemidesmosome cortical microtubule stabilizing complex tetraspanin |
author_facet |
Lisa te Molder Liesbeth Hoekman Maaike Kreft Onno Bleijerveld Arnoud Sonnenberg |
author_sort |
Lisa te Molder |
title |
Comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytes |
title_short |
Comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytes |
title_full |
Comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytes |
title_fullStr |
Comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytes |
title_full_unstemmed |
Comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytes |
title_sort |
comparative interactomics analysis reveals potential regulators of α6β4 distribution in keratinocytes |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2020-08-01 |
description |
The integrin α6β4 and cytoskeletal adaptor plectin are essential components of type I and type II hemidesmosomes (HDs). We recently identified an alternative type II HD adhesion complex that also contains CD151 and the integrin α3β1. Here, we have taken a BioID proximity labeling approach to define the proximity protein environment for α6β4 in keratinocytes. We identified 37 proteins that interacted with both α6 and β4, while 20 and 78 proteins specifically interacted with the α6 and β4 subunits, respectively. Many of the proximity interactors of α6β4 are components of focal adhesions (FAs) and the cortical microtubule stabilizing complex (CMSC). Though the close association of CMSCs with α6β4 in HDs was confirmed by immunofluorescence analysis, CMSCs have no role in the assembly of HDs. Analysis of the β4 interactome in the presence or absence of CD151 revealed that they are strikingly similar; only 11 different interactors were identified. One of these was the integrin α3β1, which interacted with α6β4 more strongly in the presence of CD151 than in its absence. These findings indicate that CD151 does not significantly contribute to the interactome of α6β4, but suggest a role of CD151 in linking α3β1 and α6β4 together in tetraspanin adhesion structures. |
topic |
integrin interactome bioid hemidesmosome cortical microtubule stabilizing complex tetraspanin |
url |
http://bio.biologists.org/content/9/8/bio054155 |
work_keys_str_mv |
AT lisatemolder comparativeinteractomicsanalysisrevealspotentialregulatorsofa6b4distributioninkeratinocytes AT liesbethhoekman comparativeinteractomicsanalysisrevealspotentialregulatorsofa6b4distributioninkeratinocytes AT maaikekreft comparativeinteractomicsanalysisrevealspotentialregulatorsofa6b4distributioninkeratinocytes AT onnobleijerveld comparativeinteractomicsanalysisrevealspotentialregulatorsofa6b4distributioninkeratinocytes AT arnoudsonnenberg comparativeinteractomicsanalysisrevealspotentialregulatorsofa6b4distributioninkeratinocytes |
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1721403511308025856 |