Proteomic Investigation of <i>S</i>-Nitrosylated Proteins During NO-Induced Adventitious Rooting of Cucumber
Nitric oxide (NO) acts an essential signaling molecule that is involved in regulating various physiological and biochemical processes in plants. However, whether <i>S</i>-nitrosylation is a crucial molecular mechanism of NO is still largely unknown. In this study, 50 μM <i>...
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doaj-8d18f23ab7004e319a69614babc69a832020-11-25T01:41:44ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-10-012021536310.3390/ijms20215363ijms20215363Proteomic Investigation of <i>S</i>-Nitrosylated Proteins During NO-Induced Adventitious Rooting of CucumberLijuan Niu0Jihua Yu1Weibiao Liao2Jianming Xie3Jian Yu4Jian Lv5Xuemei Xiao6Linli Hu7Yue Wu8College of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaNitric oxide (NO) acts an essential signaling molecule that is involved in regulating various physiological and biochemical processes in plants. However, whether <i>S</i>-nitrosylation is a crucial molecular mechanism of NO is still largely unknown. In this study, 50 μM <i>S</i>-nitrosoglutathione (GSNO) treatment was found to have a maximum biological effect on promoting adventitious rooting in cucumber. Meanwhile, removal of endogenous NO significantly inhibited the development of adventitious roots implying that NO is responsible for promoting the process of adventitious rooting. Moreover, application of GSNO resulted in an increase of intracellular <i>S</i>-nitrosothiol (SNO) levels and endogenous NO production, while decreasing the <i>S</i>-nitrosoglutathione reductase (GSNOR) activity during adventitious rooting, implicating that <i>S</i>-nitrosylation might be involved in NO-induced adventitious rooting in cucumber. Furthermore, the identification of <i>S</i>-nitrosylated proteins was performed utilizing the liquid chromatography/mass spectrometry/mass spectrometry (LC-MS/MS) and biotin-switch technique during the development of adventitious rooting. Among these proteins, the activities and <i>S</i>-nitrosylated level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), tubulin alpha chain (TUA), and glutathione reductase (GR) were further analyzed as NO direct targets. Our results indicated that NO might enhance the <i>S</i>-nitrosylation level of GAPDH and GR, and was found to subsequently reduce these activities and transcriptional levels. Conversely, <i>S</i>-nitrosylation of TUA increased the expression level of <i>TUA</i>. The results implied that <i>S</i>-nitrosylation of key proteins seems to regulate various pathways through differential <i>S</i>-nitrosylation during adventitious rooting. Collectively, these results suggest that <i>S</i>-nitrosylation could be involved in NO-induced adventitious rooting, and they also provide fundamental evidence for the molecular mechanism of NO signaling during adventitious rooting in cucumber explants.https://www.mdpi.com/1422-0067/20/21/5363nitric oxide<i>s</i>-nitrosylationadventitious root development |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lijuan Niu Jihua Yu Weibiao Liao Jianming Xie Jian Yu Jian Lv Xuemei Xiao Linli Hu Yue Wu |
spellingShingle |
Lijuan Niu Jihua Yu Weibiao Liao Jianming Xie Jian Yu Jian Lv Xuemei Xiao Linli Hu Yue Wu Proteomic Investigation of <i>S</i>-Nitrosylated Proteins During NO-Induced Adventitious Rooting of Cucumber International Journal of Molecular Sciences nitric oxide <i>s</i>-nitrosylation adventitious root development |
author_facet |
Lijuan Niu Jihua Yu Weibiao Liao Jianming Xie Jian Yu Jian Lv Xuemei Xiao Linli Hu Yue Wu |
author_sort |
Lijuan Niu |
title |
Proteomic Investigation of <i>S</i>-Nitrosylated Proteins During NO-Induced Adventitious Rooting of Cucumber |
title_short |
Proteomic Investigation of <i>S</i>-Nitrosylated Proteins During NO-Induced Adventitious Rooting of Cucumber |
title_full |
Proteomic Investigation of <i>S</i>-Nitrosylated Proteins During NO-Induced Adventitious Rooting of Cucumber |
title_fullStr |
Proteomic Investigation of <i>S</i>-Nitrosylated Proteins During NO-Induced Adventitious Rooting of Cucumber |
title_full_unstemmed |
Proteomic Investigation of <i>S</i>-Nitrosylated Proteins During NO-Induced Adventitious Rooting of Cucumber |
title_sort |
proteomic investigation of <i>s</i>-nitrosylated proteins during no-induced adventitious rooting of cucumber |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-10-01 |
description |
Nitric oxide (NO) acts an essential signaling molecule that is involved in regulating various physiological and biochemical processes in plants. However, whether <i>S</i>-nitrosylation is a crucial molecular mechanism of NO is still largely unknown. In this study, 50 μM <i>S</i>-nitrosoglutathione (GSNO) treatment was found to have a maximum biological effect on promoting adventitious rooting in cucumber. Meanwhile, removal of endogenous NO significantly inhibited the development of adventitious roots implying that NO is responsible for promoting the process of adventitious rooting. Moreover, application of GSNO resulted in an increase of intracellular <i>S</i>-nitrosothiol (SNO) levels and endogenous NO production, while decreasing the <i>S</i>-nitrosoglutathione reductase (GSNOR) activity during adventitious rooting, implicating that <i>S</i>-nitrosylation might be involved in NO-induced adventitious rooting in cucumber. Furthermore, the identification of <i>S</i>-nitrosylated proteins was performed utilizing the liquid chromatography/mass spectrometry/mass spectrometry (LC-MS/MS) and biotin-switch technique during the development of adventitious rooting. Among these proteins, the activities and <i>S</i>-nitrosylated level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), tubulin alpha chain (TUA), and glutathione reductase (GR) were further analyzed as NO direct targets. Our results indicated that NO might enhance the <i>S</i>-nitrosylation level of GAPDH and GR, and was found to subsequently reduce these activities and transcriptional levels. Conversely, <i>S</i>-nitrosylation of TUA increased the expression level of <i>TUA</i>. The results implied that <i>S</i>-nitrosylation of key proteins seems to regulate various pathways through differential <i>S</i>-nitrosylation during adventitious rooting. Collectively, these results suggest that <i>S</i>-nitrosylation could be involved in NO-induced adventitious rooting, and they also provide fundamental evidence for the molecular mechanism of NO signaling during adventitious rooting in cucumber explants. |
topic |
nitric oxide <i>s</i>-nitrosylation adventitious root development |
url |
https://www.mdpi.com/1422-0067/20/21/5363 |
work_keys_str_mv |
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