Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones
Retinal horizontal cells (HCs) feed back negatively to cone photoreceptors and in that way generate the center/surround organization of bipolar cell receptive fields. The mechanism by which HCs inhibit photoreceptors is a matter of debate. General consensus exists that horizontal cell activity leads...
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doaj-8d1986a3c3714ea18c6024aa97cdfc582020-11-24T20:47:56ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992017-12-011010.3389/fnmol.2017.00403310651Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to ConesValentina Cenedese0Wim de Graaff1Tamás Csikós2Mitali Poovayya3Georg Zoidl4Maarten Kamermans5Maarten Kamermans6Retinal Signal Processing Lab, Netherlands Institute for Neuroscience, Amsterdam, NetherlandsRetinal Signal Processing Lab, Netherlands Institute for Neuroscience, Amsterdam, NetherlandsRetinal Signal Processing Lab, Netherlands Institute for Neuroscience, Amsterdam, NetherlandsRetinal Signal Processing Lab, Netherlands Institute for Neuroscience, Amsterdam, NetherlandsDepartment of Biology, York University, Toronto, ON, CanadaRetinal Signal Processing Lab, Netherlands Institute for Neuroscience, Amsterdam, NetherlandsDepartment of Biomedical Physics and Biomedical Optics, Academic Medical Center, University of Amsterdam, Amsterdam, NetherlandsRetinal horizontal cells (HCs) feed back negatively to cone photoreceptors and in that way generate the center/surround organization of bipolar cell receptive fields. The mechanism by which HCs inhibit photoreceptors is a matter of debate. General consensus exists that horizontal cell activity leads to the modulation of the cone Ca-current. This modulation has two components, one fast and the other slow. Several mechanisms for this modulation have been proposed: a fast ephaptic mechanism, and a slow pH mediated mechanism. Here we test the hypothesis that the slow negative feedback signal from HCs to cones is mediated by Panx1 channels expressed at the tips of the dendrites of horizontal cell. We generated zebrafish lacking Panx1 and found that the slow component of the feedback signal was strongly reduced in the mutants showing that Panx1 channels are a fundamental part of the negative feedback pathway from HCs to cones.http://journal.frontiersin.org/article/10.3389/fnmol.2017.00403/fullPannexin 1retinahorizontal cellsconesfeedbackconnexins |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valentina Cenedese Wim de Graaff Tamás Csikós Mitali Poovayya Georg Zoidl Maarten Kamermans Maarten Kamermans |
spellingShingle |
Valentina Cenedese Wim de Graaff Tamás Csikós Mitali Poovayya Georg Zoidl Maarten Kamermans Maarten Kamermans Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones Frontiers in Molecular Neuroscience Pannexin 1 retina horizontal cells cones feedback connexins |
author_facet |
Valentina Cenedese Wim de Graaff Tamás Csikós Mitali Poovayya Georg Zoidl Maarten Kamermans Maarten Kamermans |
author_sort |
Valentina Cenedese |
title |
Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones |
title_short |
Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones |
title_full |
Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones |
title_fullStr |
Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones |
title_full_unstemmed |
Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones |
title_sort |
pannexin 1 is critically involved in feedback from horizontal cells to cones |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Molecular Neuroscience |
issn |
1662-5099 |
publishDate |
2017-12-01 |
description |
Retinal horizontal cells (HCs) feed back negatively to cone photoreceptors and in that way generate the center/surround organization of bipolar cell receptive fields. The mechanism by which HCs inhibit photoreceptors is a matter of debate. General consensus exists that horizontal cell activity leads to the modulation of the cone Ca-current. This modulation has two components, one fast and the other slow. Several mechanisms for this modulation have been proposed: a fast ephaptic mechanism, and a slow pH mediated mechanism. Here we test the hypothesis that the slow negative feedback signal from HCs to cones is mediated by Panx1 channels expressed at the tips of the dendrites of horizontal cell. We generated zebrafish lacking Panx1 and found that the slow component of the feedback signal was strongly reduced in the mutants showing that Panx1 channels are a fundamental part of the negative feedback pathway from HCs to cones. |
topic |
Pannexin 1 retina horizontal cells cones feedback connexins |
url |
http://journal.frontiersin.org/article/10.3389/fnmol.2017.00403/full |
work_keys_str_mv |
AT valentinacenedese pannexin1iscriticallyinvolvedinfeedbackfromhorizontalcellstocones AT wimdegraaff pannexin1iscriticallyinvolvedinfeedbackfromhorizontalcellstocones AT tamascsikos pannexin1iscriticallyinvolvedinfeedbackfromhorizontalcellstocones AT mitalipoovayya pannexin1iscriticallyinvolvedinfeedbackfromhorizontalcellstocones AT georgzoidl pannexin1iscriticallyinvolvedinfeedbackfromhorizontalcellstocones AT maartenkamermans pannexin1iscriticallyinvolvedinfeedbackfromhorizontalcellstocones AT maartenkamermans pannexin1iscriticallyinvolvedinfeedbackfromhorizontalcellstocones |
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1716809553606082560 |