Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils
This study demonstrates the possibility of using sanitizing detergents based on natural products for the elimination and/or reduction of Aeromonas hydrophila biofilm formed on stainless steel surfaces. The goal of this work was to determine the reduction effect of sanitizing detergents containing es...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
2013-01-01
|
Series: | Brazilian Journal of Microbiology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100010 |
id |
doaj-8d20a4b69bee48e481e3edc21ecaf053 |
---|---|
record_format |
Article |
spelling |
doaj-8d20a4b69bee48e481e3edc21ecaf0532020-11-24T20:46:46ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052013-01-014417380Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oilsAlessandra Farias MilleziMaria das Graças CardosoEduardo AlvesRoberta Hilsdorf PiccoliThis study demonstrates the possibility of using sanitizing detergents based on natural products for the elimination and/or reduction of Aeromonas hydrophila biofilm formed on stainless steel surfaces. The goal of this work was to determine the reduction effect of sanitizing detergents containing essential oils of Thymus vulgaris (thyme) and Cymbopogon citratus (lemongrass) on biofilm formed by A. hydrophila on AISI 304 stainless steel coupons, using UHT skimmed milk as substratum. There was adhesion and biofilm formation by A. hydrophila at 28 ºC, presenting 7.60 log cfu.cm-2 after the fourth day of cultivation. There was no significant difference between the lemongrass treatment and that of the thyme oil (p < 0.05). However, both treatments significantly reduced the biofilm, differing significantly from the NaOH control (p > 0.05). The treatment with lemongrass solution reduced the biofilm by 4.51 log cfu cm-2 at 25 ºC. The thyme detergent also reduced the number of cfu cm-2 by 3.84 log cycles at 25 ºC. The use of the lemongrass and thyme solutions efficiently reduced the A. hydrophila biofilm.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100010Thymus vulgarisCymbopogon citratusnatural disinfectantssanitation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alessandra Farias Millezi Maria das Graças Cardoso Eduardo Alves Roberta Hilsdorf Piccoli |
spellingShingle |
Alessandra Farias Millezi Maria das Graças Cardoso Eduardo Alves Roberta Hilsdorf Piccoli Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils Brazilian Journal of Microbiology Thymus vulgaris Cymbopogon citratus natural disinfectants sanitation |
author_facet |
Alessandra Farias Millezi Maria das Graças Cardoso Eduardo Alves Roberta Hilsdorf Piccoli |
author_sort |
Alessandra Farias Millezi |
title |
Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils |
title_short |
Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils |
title_full |
Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils |
title_fullStr |
Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils |
title_full_unstemmed |
Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils |
title_sort |
reduction of aeromonas hidrophyla biofilm on stainless stell surface by essential oils |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1517-8382 1678-4405 |
publishDate |
2013-01-01 |
description |
This study demonstrates the possibility of using sanitizing detergents based on natural products for the elimination and/or reduction of Aeromonas hydrophila biofilm formed on stainless steel surfaces. The goal of this work was to determine the reduction effect of sanitizing detergents containing essential oils of Thymus vulgaris (thyme) and Cymbopogon citratus (lemongrass) on biofilm formed by A. hydrophila on AISI 304 stainless steel coupons, using UHT skimmed milk as substratum. There was adhesion and biofilm formation by A. hydrophila at 28 ºC, presenting 7.60 log cfu.cm-2 after the fourth day of cultivation. There was no significant difference between the lemongrass treatment and that of the thyme oil (p < 0.05). However, both treatments significantly reduced the biofilm, differing significantly from the NaOH control (p > 0.05). The treatment with lemongrass solution reduced the biofilm by 4.51 log cfu cm-2 at 25 ºC. The thyme detergent also reduced the number of cfu cm-2 by 3.84 log cycles at 25 ºC. The use of the lemongrass and thyme solutions efficiently reduced the A. hydrophila biofilm. |
topic |
Thymus vulgaris Cymbopogon citratus natural disinfectants sanitation |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100010 |
work_keys_str_mv |
AT alessandrafariasmillezi reductionofaeromonashidrophylabiofilmonstainlessstellsurfacebyessentialoils AT mariadasgracascardoso reductionofaeromonashidrophylabiofilmonstainlessstellsurfacebyessentialoils AT eduardoalves reductionofaeromonashidrophylabiofilmonstainlessstellsurfacebyessentialoils AT robertahilsdorfpiccoli reductionofaeromonashidrophylabiofilmonstainlessstellsurfacebyessentialoils |
_version_ |
1716811574065233920 |