Usefulness of a Kampo Medicine on Stress-Induced Delayed Gastric Emptying in Mice
Anxiety and depression often occur with gastrointestinal symptoms. Although the Japanese traditional medicine (Kampo medicine) bukuryoingohangekobokuto (BGH) is approved for treating anxiety, neurotic gastritis, and heartburn, its effect on gastrointestinal motility remains poorly known. This study...
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doaj-8d402768128546b4a10f831a5e1bdc192020-11-25T02:40:31ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882020-01-01202010.1155/2020/37972193797219Usefulness of a Kampo Medicine on Stress-Induced Delayed Gastric Emptying in MiceSachiko Mogami0Ryutaro Arita1Miwa Nahata2Naoki Fujitsuka3Shin Takayama4Tadashi Ishii5Tsumura Kampo Research Laboratories, Tsumura & Co., Ibaraki, JapanDepartment of Education and Support for Regional Medicine, Department of Kampo Medicine, Tohoku University Hospital, Sendai, Miyagi 980-8574, JapanTsumura Kampo Research Laboratories, Tsumura & Co., Ibaraki, JapanTsumura Kampo Research Laboratories, Tsumura & Co., Ibaraki, JapanDepartment of Education and Support for Regional Medicine, Department of Kampo Medicine, Tohoku University Hospital, Sendai, Miyagi 980-8574, JapanDepartment of Education and Support for Regional Medicine, Department of Kampo Medicine, Tohoku University Hospital, Sendai, Miyagi 980-8574, JapanAnxiety and depression often occur with gastrointestinal symptoms. Although the Japanese traditional medicine (Kampo medicine) bukuryoingohangekobokuto (BGH) is approved for treating anxiety, neurotic gastritis, and heartburn, its effect on gastrointestinal motility remains poorly known. This study aimed to examine the effect of BGH on delayed gastric emptying in stress model mice and clarified its action mechanism. Seven-week-old C57BL/6 male mice were acclimated for a week and fasted overnight. Stress hormone, corticotropin-releasing factor (CRF), was intracerebroventricularly injected to mice, and solid nutrient meal (ground chow and distilled water) was orally administered 1 hour after. Gastric contents were collected to evaluate gastric emptying rates by measuring its dry weight. Injection of CRF (0.3 or 1.0 μg/mouse) significantly delayed the 2-hour gastric emptying in mice. BGH (1.0 g/kg), which was administered 30 minutes before the CRF injection, significantly ameliorated the delayed gastric emptying induced by CRF (0.3 μg/mouse). BGH (0.5, 1.0 g/kg) significantly enhanced the 1-hour gastric emptying and slightly increased the 2-hour gastric emptying in mice without CRF injection. In vitro functional assays showed that components of BGH antagonized or inhibited CRF type-2, dopamine D2/D3, neuropeptide Y Y2 receptors, or acetylcholinesterase. In conclusion, the components of BGH may exert synergistic effects on improving gastric emptying via various targets. BGH is considered to be potentially useful for treating gastrointestinal dysmotility with psychological symptoms.http://dx.doi.org/10.1155/2020/3797219 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sachiko Mogami Ryutaro Arita Miwa Nahata Naoki Fujitsuka Shin Takayama Tadashi Ishii |
spellingShingle |
Sachiko Mogami Ryutaro Arita Miwa Nahata Naoki Fujitsuka Shin Takayama Tadashi Ishii Usefulness of a Kampo Medicine on Stress-Induced Delayed Gastric Emptying in Mice Evidence-Based Complementary and Alternative Medicine |
author_facet |
Sachiko Mogami Ryutaro Arita Miwa Nahata Naoki Fujitsuka Shin Takayama Tadashi Ishii |
author_sort |
Sachiko Mogami |
title |
Usefulness of a Kampo Medicine on Stress-Induced Delayed Gastric Emptying in Mice |
title_short |
Usefulness of a Kampo Medicine on Stress-Induced Delayed Gastric Emptying in Mice |
title_full |
Usefulness of a Kampo Medicine on Stress-Induced Delayed Gastric Emptying in Mice |
title_fullStr |
Usefulness of a Kampo Medicine on Stress-Induced Delayed Gastric Emptying in Mice |
title_full_unstemmed |
Usefulness of a Kampo Medicine on Stress-Induced Delayed Gastric Emptying in Mice |
title_sort |
usefulness of a kampo medicine on stress-induced delayed gastric emptying in mice |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2020-01-01 |
description |
Anxiety and depression often occur with gastrointestinal symptoms. Although the Japanese traditional medicine (Kampo medicine) bukuryoingohangekobokuto (BGH) is approved for treating anxiety, neurotic gastritis, and heartburn, its effect on gastrointestinal motility remains poorly known. This study aimed to examine the effect of BGH on delayed gastric emptying in stress model mice and clarified its action mechanism. Seven-week-old C57BL/6 male mice were acclimated for a week and fasted overnight. Stress hormone, corticotropin-releasing factor (CRF), was intracerebroventricularly injected to mice, and solid nutrient meal (ground chow and distilled water) was orally administered 1 hour after. Gastric contents were collected to evaluate gastric emptying rates by measuring its dry weight. Injection of CRF (0.3 or 1.0 μg/mouse) significantly delayed the 2-hour gastric emptying in mice. BGH (1.0 g/kg), which was administered 30 minutes before the CRF injection, significantly ameliorated the delayed gastric emptying induced by CRF (0.3 μg/mouse). BGH (0.5, 1.0 g/kg) significantly enhanced the 1-hour gastric emptying and slightly increased the 2-hour gastric emptying in mice without CRF injection. In vitro functional assays showed that components of BGH antagonized or inhibited CRF type-2, dopamine D2/D3, neuropeptide Y Y2 receptors, or acetylcholinesterase. In conclusion, the components of BGH may exert synergistic effects on improving gastric emptying via various targets. BGH is considered to be potentially useful for treating gastrointestinal dysmotility with psychological symptoms. |
url |
http://dx.doi.org/10.1155/2020/3797219 |
work_keys_str_mv |
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