Bioremediation of cooking oil waste using lipases from wastes.
Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, alt...
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doaj-8d51fbfc3a0049c6bb74b240f8f0792e2020-11-24T22:05:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011210e018624610.1371/journal.pone.0186246Bioremediation of cooking oil waste using lipases from wastes.Clarissa Hamaio Okino-DelgadoDébora Zanoni do PradoRoselaine FacanaliMárcia Mayo Ortiz MarquesAugusto Santana NascimentoCélio Junior da Costa FernandesWilliam Fernando ZambuzziLuciana Francisco FleuriCooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases.http://europepmc.org/articles/PMC5657992?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Clarissa Hamaio Okino-Delgado Débora Zanoni do Prado Roselaine Facanali Márcia Mayo Ortiz Marques Augusto Santana Nascimento Célio Junior da Costa Fernandes William Fernando Zambuzzi Luciana Francisco Fleuri |
spellingShingle |
Clarissa Hamaio Okino-Delgado Débora Zanoni do Prado Roselaine Facanali Márcia Mayo Ortiz Marques Augusto Santana Nascimento Célio Junior da Costa Fernandes William Fernando Zambuzzi Luciana Francisco Fleuri Bioremediation of cooking oil waste using lipases from wastes. PLoS ONE |
author_facet |
Clarissa Hamaio Okino-Delgado Débora Zanoni do Prado Roselaine Facanali Márcia Mayo Ortiz Marques Augusto Santana Nascimento Célio Junior da Costa Fernandes William Fernando Zambuzzi Luciana Francisco Fleuri |
author_sort |
Clarissa Hamaio Okino-Delgado |
title |
Bioremediation of cooking oil waste using lipases from wastes. |
title_short |
Bioremediation of cooking oil waste using lipases from wastes. |
title_full |
Bioremediation of cooking oil waste using lipases from wastes. |
title_fullStr |
Bioremediation of cooking oil waste using lipases from wastes. |
title_full_unstemmed |
Bioremediation of cooking oil waste using lipases from wastes. |
title_sort |
bioremediation of cooking oil waste using lipases from wastes. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases. |
url |
http://europepmc.org/articles/PMC5657992?pdf=render |
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