Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice
CRISPR/Cas9 technology has been used for genome engineering in vivo. Here, the authors use a transfection technique to deliver multiple guide RNAs to the pancreas of adult mice, allowing genetic screening and chromosome engineering in pancreatic cancer.
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2016-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms10770 |
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doaj-8d54419326794a6ba55ff4332e373a042021-05-11T10:57:00ZengNature Publishing GroupNature Communications2041-17232016-02-017111310.1038/ncomms10770Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in miceRoman Maresch0Sebastian Mueller1Christian Veltkamp2Rupert Öllinger3Mathias Friedrich4Irina Heid5Katja Steiger6Julia Weber7Thomas Engleitner8Maxim Barenboim9Sabine Klein10Sandra Louzada11Ruby Banerjee12Alexander Strong13Teresa Stauber14Nina Gross15Ulf Geumann16Sebastian Lange17Marc Ringelhan18Ignacio Varela19Kristian Unger20Fengtang Yang21Roland M. Schmid22George S. Vassiliou23Rickmer Braren24Günter Schneider25Mathias Heikenwalder26Allan Bradley27Dieter Saur28Roland Rad29Department of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenWellcome Trust Sanger Institute, Wellcome Trust Genome CampusInstitute of Radiology, Klinikum rechts der Isar, Technischen Universität MünchenDepartment of Pathology, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenWellcome Trust Sanger Institute, Wellcome Trust Genome CampusWellcome Trust Sanger Institute, Wellcome Trust Genome CampusWellcome Trust Sanger Institute, Wellcome Trust Genome CampusDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenInstituto de Biomedicina y Biotecnología de CantabriaHelmholtz Zentrum München, Research Unit Radiation CytogeneticsWellcome Trust Sanger Institute, Wellcome Trust Genome CampusDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenWellcome Trust Sanger Institute, Wellcome Trust Genome CampusInstitute of Radiology, Klinikum rechts der Isar, Technischen Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenInstitute of Virology, Technische Universität München/Helmholtz Zentrum MünchenWellcome Trust Sanger Institute, Wellcome Trust Genome CampusDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenDepartment of Medicine II, Klinikum rechts der Isar, Technische Universität MünchenCRISPR/Cas9 technology has been used for genome engineering in vivo. Here, the authors use a transfection technique to deliver multiple guide RNAs to the pancreas of adult mice, allowing genetic screening and chromosome engineering in pancreatic cancer.https://doi.org/10.1038/ncomms10770 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Roman Maresch Sebastian Mueller Christian Veltkamp Rupert Öllinger Mathias Friedrich Irina Heid Katja Steiger Julia Weber Thomas Engleitner Maxim Barenboim Sabine Klein Sandra Louzada Ruby Banerjee Alexander Strong Teresa Stauber Nina Gross Ulf Geumann Sebastian Lange Marc Ringelhan Ignacio Varela Kristian Unger Fengtang Yang Roland M. Schmid George S. Vassiliou Rickmer Braren Günter Schneider Mathias Heikenwalder Allan Bradley Dieter Saur Roland Rad |
| spellingShingle |
Roman Maresch Sebastian Mueller Christian Veltkamp Rupert Öllinger Mathias Friedrich Irina Heid Katja Steiger Julia Weber Thomas Engleitner Maxim Barenboim Sabine Klein Sandra Louzada Ruby Banerjee Alexander Strong Teresa Stauber Nina Gross Ulf Geumann Sebastian Lange Marc Ringelhan Ignacio Varela Kristian Unger Fengtang Yang Roland M. Schmid George S. Vassiliou Rickmer Braren Günter Schneider Mathias Heikenwalder Allan Bradley Dieter Saur Roland Rad Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice Nature Communications |
| author_facet |
Roman Maresch Sebastian Mueller Christian Veltkamp Rupert Öllinger Mathias Friedrich Irina Heid Katja Steiger Julia Weber Thomas Engleitner Maxim Barenboim Sabine Klein Sandra Louzada Ruby Banerjee Alexander Strong Teresa Stauber Nina Gross Ulf Geumann Sebastian Lange Marc Ringelhan Ignacio Varela Kristian Unger Fengtang Yang Roland M. Schmid George S. Vassiliou Rickmer Braren Günter Schneider Mathias Heikenwalder Allan Bradley Dieter Saur Roland Rad |
| author_sort |
Roman Maresch |
| title |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_short |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_full |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_fullStr |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_full_unstemmed |
Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice |
| title_sort |
multiplexed pancreatic genome engineering and cancer induction by transfection-based crispr/cas9 delivery in mice |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2016-02-01 |
| description |
CRISPR/Cas9 technology has been used for genome engineering in vivo. Here, the authors use a transfection technique to deliver multiple guide RNAs to the pancreas of adult mice, allowing genetic screening and chromosome engineering in pancreatic cancer. |
| url |
https://doi.org/10.1038/ncomms10770 |
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