Surface‐bound matrix metalloproteinase‐8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriers
ABSTRACT Objective MMP‐8 binds to surface‐bound tissue inhibitor of metalloproteinase‐1 (TIMP‐1) on PMNs to promote pericellular proteolysis during the development of inflammatory diseases associated with tissue destruction. Little is known about the biology of MMP‐8 in macrophages. We tested the hy...
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doaj-8d6f48bfb98641829479006e4f5ea4eb2021-03-13T18:00:36ZengWileyPhysiological Reports2051-817X2021-03-0195n/an/a10.14814/phy2.14778Surface‐bound matrix metalloproteinase‐8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriersXiaoyun Wang0Duo Zhang1Quynh‐Anh Fucci2Clare M. Dollery3Caroline A. Owen4Division of Pulmonary and Critical Care Medicine Brigham and Women's Hospital and Harvard Medical School Boston MA USAClinical and Experimental Therapeutics College of Pharmacy University of Georgia and Charlie Norwood VA Medical Center Augusta GA USADivision of Pulmonary and Critical Care Medicine Brigham and Women's Hospital and Harvard Medical School Boston MA USAWhittington HospitalWittington Health NHS Trust London UKDivision of Pulmonary and Critical Care Medicine Brigham and Women's Hospital and Harvard Medical School Boston MA USAABSTRACT Objective MMP‐8 binds to surface‐bound tissue inhibitor of metalloproteinase‐1 (TIMP‐1) on PMNs to promote pericellular proteolysis during the development of inflammatory diseases associated with tissue destruction. Little is known about the biology of MMP‐8 in macrophages. We tested the hypotheses that: (1) MMP‐8 and TIMP‐1 are also expressed on the surface of activated macrophages, (2) surface‐bound MMP‐8 on macrophages promotes TIMP‐resistant pericellular proteolysis and macrophage migration through tissue barriers, and (3) MMP‐8 binds to surface‐bound TIMP‐1 on macrophages. Methods Surface MMP‐8 and TIMP‐1 levels were measured on human monocyte‐derived macrophages (MDM) and/or murine macrophages using immunostaining, biotin‐labeling, and substrate cleavage methods. The susceptibility of membrane‐bound Mmp‐8 on activated macrophages from wild‐type (WT) mice to TIMPs was measured. Migration of WT and Mmp‐8−/− macrophages through models of tissue barriers in vitro and the accumulation of peritoneal macrophages in WT versus Mmp‐8−/− mice with sterile peritonitis was compared. Surface levels of Mmp‐8 were compared on activated macrophages from WT and Timp‐1−/− mice. Results Lipopolysaccharides and a cluster of differentiation 40 ligand increased surface MMP‐8 and/or TIMP‐1 staining and surface type I collagenase activity on MDM and/or murine macrophages. Activated Mmp‐8−/− macrophages degraded less type I collagen than activated WT macrophages. The surface type‐I collagenase activity on WT macrophages was resistant to inhibition by Timp‐1. Peritoneal macrophage accumulation was similar in WT and Mmp‐8−/− mice with sterile acute peritonitis. However, Mmp‐8−/− macrophages migrated less efficiently through models of tissue barriers (especially those containing type I collagen) than WT cells. Activated WT and Timp‐1−/− macrophages had similar surface‐bound Mmp‐8 levels. Conclusions MMP‐8 and TIMP‐1 are expressed on the surface of activated human MDM and murine macrophages, but Mmp‐8 is unlikely to bind to surface‐bound Timp‐1 on these cells. Surface‐bound MMP‐8 contributes to TIMP‐resistant monocyte/macrophage pericellular proteolysis and macrophage migration through collagen‐containing tissue barriers.https://doi.org/10.14814/phy2.14778acute lung injurychronic obstructive pulmonary diseasefibrosisinterstitial collagenproteolysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoyun Wang Duo Zhang Quynh‐Anh Fucci Clare M. Dollery Caroline A. Owen |
spellingShingle |
Xiaoyun Wang Duo Zhang Quynh‐Anh Fucci Clare M. Dollery Caroline A. Owen Surface‐bound matrix metalloproteinase‐8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriers Physiological Reports acute lung injury chronic obstructive pulmonary disease fibrosis interstitial collagen proteolysis |
author_facet |
Xiaoyun Wang Duo Zhang Quynh‐Anh Fucci Clare M. Dollery Caroline A. Owen |
author_sort |
Xiaoyun Wang |
title |
Surface‐bound matrix metalloproteinase‐8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriers |
title_short |
Surface‐bound matrix metalloproteinase‐8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriers |
title_full |
Surface‐bound matrix metalloproteinase‐8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriers |
title_fullStr |
Surface‐bound matrix metalloproteinase‐8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriers |
title_full_unstemmed |
Surface‐bound matrix metalloproteinase‐8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriers |
title_sort |
surface‐bound matrix metalloproteinase‐8 on macrophages: contributions to macrophage pericellular proteolysis and migration through tissue barriers |
publisher |
Wiley |
series |
Physiological Reports |
issn |
2051-817X |
publishDate |
2021-03-01 |
description |
ABSTRACT Objective MMP‐8 binds to surface‐bound tissue inhibitor of metalloproteinase‐1 (TIMP‐1) on PMNs to promote pericellular proteolysis during the development of inflammatory diseases associated with tissue destruction. Little is known about the biology of MMP‐8 in macrophages. We tested the hypotheses that: (1) MMP‐8 and TIMP‐1 are also expressed on the surface of activated macrophages, (2) surface‐bound MMP‐8 on macrophages promotes TIMP‐resistant pericellular proteolysis and macrophage migration through tissue barriers, and (3) MMP‐8 binds to surface‐bound TIMP‐1 on macrophages. Methods Surface MMP‐8 and TIMP‐1 levels were measured on human monocyte‐derived macrophages (MDM) and/or murine macrophages using immunostaining, biotin‐labeling, and substrate cleavage methods. The susceptibility of membrane‐bound Mmp‐8 on activated macrophages from wild‐type (WT) mice to TIMPs was measured. Migration of WT and Mmp‐8−/− macrophages through models of tissue barriers in vitro and the accumulation of peritoneal macrophages in WT versus Mmp‐8−/− mice with sterile peritonitis was compared. Surface levels of Mmp‐8 were compared on activated macrophages from WT and Timp‐1−/− mice. Results Lipopolysaccharides and a cluster of differentiation 40 ligand increased surface MMP‐8 and/or TIMP‐1 staining and surface type I collagenase activity on MDM and/or murine macrophages. Activated Mmp‐8−/− macrophages degraded less type I collagen than activated WT macrophages. The surface type‐I collagenase activity on WT macrophages was resistant to inhibition by Timp‐1. Peritoneal macrophage accumulation was similar in WT and Mmp‐8−/− mice with sterile acute peritonitis. However, Mmp‐8−/− macrophages migrated less efficiently through models of tissue barriers (especially those containing type I collagen) than WT cells. Activated WT and Timp‐1−/− macrophages had similar surface‐bound Mmp‐8 levels. Conclusions MMP‐8 and TIMP‐1 are expressed on the surface of activated human MDM and murine macrophages, but Mmp‐8 is unlikely to bind to surface‐bound Timp‐1 on these cells. Surface‐bound MMP‐8 contributes to TIMP‐resistant monocyte/macrophage pericellular proteolysis and macrophage migration through collagen‐containing tissue barriers. |
topic |
acute lung injury chronic obstructive pulmonary disease fibrosis interstitial collagen proteolysis |
url |
https://doi.org/10.14814/phy2.14778 |
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