Synergism of components as the main approach to creation of a probiotic complex

• Main Authors: E. V. Shikh, S. A. Solovieva, A. V. Perkov
• Format: Article
• Language: Russian
• Published: Remedium Group LLC 2020-05-01
• Series: Медицинский совет
• Subjects: bifidobacteria; lactobacilli; inulin; cobalamin; corrinoids
• Online Access: https://www.med-sovet.pro/jour/article/view/5611

Development of probiotic products containing several strains: Bifidumbacterium lactis BI-04, Lactobacillus acidophilus La-14 and Lactobacillus rhamnosus Lr-32, is an upcoming trend, as it builds capacity of preventive treatment. According to the results of in vitro experimental studies, the strains of Lactobacillus acidophilus La-14, L. rhamnosus Lr-32, Bifidumbacterium lactis BI-04 are resistant to low pH conditions and survive in the concentrations of bile salts in the small intestine. Demonstrate high adhesion to the human epithelial cell lines - Caco-2 and HT-29. In vitro experiments have shown the inhibitory effect of strains L. acidophilus La-14, L. rhamnosus Lr-32 on the following pathogens: Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes. The probiotic strains with in vitro and in vivo confirmed effects combined a prebiotic and B vitamins is considered a rational combination. Additional favourable conditions in the form of nutrient medium (prebiotic), sufficient energy supply (coenzyme vitamins) contribute towards the increase of colonization potential. Inulin and B vitamins have their own activity, which is synergistic with the probiotic microorganism activity. Vitamin B12 has a porphyrin-like or corrine ring in its structure, which is linked to the cobalt atom by four coordination bonds and is representative of corrinoids group. Microorganisms inhabiting the GI tract use exogenously produced cobalamin corrinoids as cofactors. It is appropriate to add the use of probiotics with additional exogenous intake of vitamin B12, as microbial communities of the human gut are likely competitors in the consumption of cobalamin. The rational selection of components contributes to the maximum implementation of potential preventive effects of probiotic microorganisms.