| Summary: | In a hypogonadal rat model, calcium ATPase (Ca2+-ATPase) activity and membrane fluidity were examined in plasma membrane of duodenal enterocytes under two different stress conditions developed by chemical change (lipid saturation) and physical change (temperature). Data generated show that, under both these stress conditions, calcium ATPase activity was decreased. Membrane fluidity (phase transition temperature) study by fluorescence polarization measurement indicated that, compared to corresponding control enterocyte membranes, differences were seen in the phase transition temperature (Tc) of the oophorectomized rats’ enterocyte membrane under both the conditions of stress, which suggested a decrease in membrane fluidity under both the experimental situations. It is proposed that the degree of alteration in calcium ATPase activity in oophorectomized rats’ duodenal enterocyte plasma membrane under two different stress conditions was nearly alike, which was influenced by changes in membrane fluidity induced by chemical change (lipid saturation) or physical change (temperature).
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