Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture
Objectives: Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropr...
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Tehran University of Medical Sciences
2017-10-01
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Series: | Frontiers in Dentistry |
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Online Access: | https://jdt.tums.ac.ir/index.php/jdt/article/view/1904 |
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language |
English |
format |
Article |
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author |
Meysam Ganjibakhsh Pouyan Aminishakib Parvaneh Farzaneh Abbas Karimi Seyed Abolhassan Shahzadeh Fazeli Moones Rajabi Ahmad Nasimian Fereshteh Baghaei Naini Hedieh Rahmati Neda sadat Gohari Nazanin Mohebali Masoumeh Asadi Zahra Elyasi Gorji Mehrnaz Izadpanah Shiva Mohamadi Moghanjoghi Sepideh Ashouri |
spellingShingle |
Meysam Ganjibakhsh Pouyan Aminishakib Parvaneh Farzaneh Abbas Karimi Seyed Abolhassan Shahzadeh Fazeli Moones Rajabi Ahmad Nasimian Fereshteh Baghaei Naini Hedieh Rahmati Neda sadat Gohari Nazanin Mohebali Masoumeh Asadi Zahra Elyasi Gorji Mehrnaz Izadpanah Shiva Mohamadi Moghanjoghi Sepideh Ashouri Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture Frontiers in Dentistry Carcinoma Squamous Cell of Head and Neck Primary Cell Culture Mouth Neoplasms |
author_facet |
Meysam Ganjibakhsh Pouyan Aminishakib Parvaneh Farzaneh Abbas Karimi Seyed Abolhassan Shahzadeh Fazeli Moones Rajabi Ahmad Nasimian Fereshteh Baghaei Naini Hedieh Rahmati Neda sadat Gohari Nazanin Mohebali Masoumeh Asadi Zahra Elyasi Gorji Mehrnaz Izadpanah Shiva Mohamadi Moghanjoghi Sepideh Ashouri |
author_sort |
Meysam Ganjibakhsh |
title |
Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture |
title_short |
Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture |
title_full |
Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture |
title_fullStr |
Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture |
title_full_unstemmed |
Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture |
title_sort |
establishment and characterization of primary cultures from iranian oral squamous cell carcinoma patients by enzymatic method and explant culture |
publisher |
Tehran University of Medical Sciences |
series |
Frontiers in Dentistry |
issn |
2676-296X |
publishDate |
2017-10-01 |
description |
Objectives: Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropriate tool for such researches. Cancer cell culture is a useful tool for investigations on biochemical, genetic, molecular and immunological characteristics of different cancers, including oral cancer. Here, we explain the establishment process of five primary oral cancer cells derived from an Iranian population.
Materials and Methods: The specimens were obtained from five oral cancer patients. Enzymatic, explant culture and magnetic-activated cell sorting (MACS) methods were used for cell isolation. After quality control tests, characterization and authentication of primary oral cancer cells were performed by short tandem repeats (STR) profiling, chromosome analysis, species identification, and monitoring the growth, morphology and the expression of CD326 and CD133 markers.
Results: Five primary oral cancer cells were established from an Iranian population. The flow cytometry results showed that the isolated cells were positive for CD326 and CD133 markers. Furthermore, the cells were free from mycoplasma, bacterial and fungal contamination. No misidentified or cross-contaminated cells were detected by STR analysis.
Conclusions: Human primary oral cancer cells provide an extremely useful platform for studying carcinogenesis pathways of oral cancer in Iranian population. They may be helpful in explaining the ethnic differences in cancer biology and the individuality in anticancer drug response in future studies.
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topic |
Carcinoma Squamous Cell of Head and Neck Primary Cell Culture Mouth Neoplasms |
url |
https://jdt.tums.ac.ir/index.php/jdt/article/view/1904 |
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doaj-8d80a573694c48d4828e74f4b6c5c6732020-11-25T03:40:09ZengTehran University of Medical SciencesFrontiers in Dentistry2676-296X2017-10-01144926Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant CultureMeysam Ganjibakhsh0Pouyan Aminishakib1Parvaneh Farzaneh2Abbas Karimi3Seyed Abolhassan Shahzadeh Fazeli4Moones Rajabi5Ahmad Nasimian6Fereshteh Baghaei Naini7Hedieh Rahmati8Neda sadat Gohari9Nazanin Mohebali10Masoumeh Asadi11Zahra Elyasi Gorji12Mehrnaz Izadpanah13Shiva Mohamadi Moghanjoghi14Sepideh Ashouri15PhD Student, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, IranAssistant Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranAssistant Professor, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, IranAssistant Professor, Craniomaxillofacial Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranAssociate Professor, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran; Department of Molecular and Cellular Biology, School of Basic Science and Advanced Technologies in Biology, University of Sciences and Culture, Tehran, IranAdjunct Assistant Professor, Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranPhD Student, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran; Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modares University, Tehran, IranAssociate Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranResearcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, IranResearcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, IranResearcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, IranResearcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, IranResearcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, IranPhD Student, Department of Applied Cell Science and Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranResearcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, IranResearcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran Objectives: Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropriate tool for such researches. Cancer cell culture is a useful tool for investigations on biochemical, genetic, molecular and immunological characteristics of different cancers, including oral cancer. Here, we explain the establishment process of five primary oral cancer cells derived from an Iranian population. Materials and Methods: The specimens were obtained from five oral cancer patients. Enzymatic, explant culture and magnetic-activated cell sorting (MACS) methods were used for cell isolation. After quality control tests, characterization and authentication of primary oral cancer cells were performed by short tandem repeats (STR) profiling, chromosome analysis, species identification, and monitoring the growth, morphology and the expression of CD326 and CD133 markers. Results: Five primary oral cancer cells were established from an Iranian population. The flow cytometry results showed that the isolated cells were positive for CD326 and CD133 markers. Furthermore, the cells were free from mycoplasma, bacterial and fungal contamination. No misidentified or cross-contaminated cells were detected by STR analysis. Conclusions: Human primary oral cancer cells provide an extremely useful platform for studying carcinogenesis pathways of oral cancer in Iranian population. They may be helpful in explaining the ethnic differences in cancer biology and the individuality in anticancer drug response in future studies. https://jdt.tums.ac.ir/index.php/jdt/article/view/1904CarcinomaSquamous Cell of Head and NeckPrimary Cell CultureMouth Neoplasms |