Locus-Specific Proteomics by TChP: Targeted Chromatin Purification

Locus-Specific Proteomics by TChP: Targeted Chromatin Purification

Here, we show that transcription factors bound to regulatory sequences can be identified by purifying these unique sequences directly from mammalian cells in vivo. Using targeted chromatin purification (TChP), a double-pull-down strategy with a tetracycline-sensitive “hook” bound to a specific prom...

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Bibliographic Details
Main Authors: Farzin Pourfarzad, Ali Aghajanirefah, Ernie de Boer, Sara Ten Have, Thamar Bryn van Dijk, Sima Kheradmandkia, Ralph Stadhouders, Supat Thongjuea, Eric Soler, Nynke Gillemans, Marieke von Lindern, Jeroen Demmers, Sjaak Philipsen, Frank Grosveld
Format: Article
Language:English
Published: Elsevier 2013-08-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124713003513

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