MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine
A method of direct lipid analysis by MALDI mass spectrometry in intact membranes, without prior extraction/separation steps, is described. The purple membrane isolated from the extremely halophilic archaeon Halobacterium salinarum was selected as model membrane. Lyophilized purple membrane were grin...
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2010-09-01
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doaj-8da74e1c647547e49420fcf50ff99c202021-04-28T06:06:20ZengElsevierJournal of Lipid Research0022-22752010-09-0151928182825MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridineRoberto Angelini0Francesco Babudri1Simona Lobasso2Angela Corcelli3Department of Medical Biochemistry, Biology and Physics, University of Bari Aldo Moro, ItalyDepartment of Chemistry, University of Bari Aldo Moro, ItalyDepartment of Medical Biochemistry, Biology and Physics, University of Bari Aldo Moro, ItalyTo whom correspondence should be addressed. a.corcelli@biologia.uniba.it; Department of Medical Biochemistry, Biology and Physics, University of Bari Aldo Moro, Italy; Institute for Chemical-Physical Processes, National Research Council (IPCF-CNR), Bari, ItalyA method of direct lipid analysis by MALDI mass spectrometry in intact membranes, without prior extraction/separation steps, is described. The purple membrane isolated from the extremely halophilic archaeon Halobacterium salinarum was selected as model membrane. Lyophilized purple membrane were grinded with 9-aminoacridine (9-AA) as dry matrix, and the powder mixture was crushed in a mechanical die press to form a thin pellet. Small pieces of the pellet were then attached to the MALDI target and directly analyzed. In parallel, individual archaebacterial phospholipids and glycolipids, together with the total lipid extract of the purple membrane, were analyzed by MALDI-TOF/MS using 9-AA as the matrix in solution. Results show that 9-AA represents a suitable matrix for the conventional MALDI-TOF/MS analysis of lipid extracts from archaeal microorganisms, as well as for fast and reliable direct dry lipid analysis of lyophilized archaebacterial membranes. This method might be of general application, offering the advantage of quickly gaining information about lipid components without disrupting or altering the membrane matrix.http://www.sciencedirect.com/science/article/pii/S0022227520423120glycerolipids (diphytanylglycerol ether analogs)glycerophospholipids (diphytanylglycerolphosphate ether analogs)Halobacterium salinarumpurple membranematrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roberto Angelini Francesco Babudri Simona Lobasso Angela Corcelli |
spellingShingle |
Roberto Angelini Francesco Babudri Simona Lobasso Angela Corcelli MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine Journal of Lipid Research glycerolipids (diphytanylglycerol ether analogs) glycerophospholipids (diphytanylglycerolphosphate ether analogs) Halobacterium salinarum purple membrane matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
author_facet |
Roberto Angelini Francesco Babudri Simona Lobasso Angela Corcelli |
author_sort |
Roberto Angelini |
title |
MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine |
title_short |
MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine |
title_full |
MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine |
title_fullStr |
MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine |
title_full_unstemmed |
MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine |
title_sort |
maldi-tof/ms analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2010-09-01 |
description |
A method of direct lipid analysis by MALDI mass spectrometry in intact membranes, without prior extraction/separation steps, is described. The purple membrane isolated from the extremely halophilic archaeon Halobacterium salinarum was selected as model membrane. Lyophilized purple membrane were grinded with 9-aminoacridine (9-AA) as dry matrix, and the powder mixture was crushed in a mechanical die press to form a thin pellet. Small pieces of the pellet were then attached to the MALDI target and directly analyzed. In parallel, individual archaebacterial phospholipids and glycolipids, together with the total lipid extract of the purple membrane, were analyzed by MALDI-TOF/MS using 9-AA as the matrix in solution. Results show that 9-AA represents a suitable matrix for the conventional MALDI-TOF/MS analysis of lipid extracts from archaeal microorganisms, as well as for fast and reliable direct dry lipid analysis of lyophilized archaebacterial membranes. This method might be of general application, offering the advantage of quickly gaining information about lipid components without disrupting or altering the membrane matrix. |
topic |
glycerolipids (diphytanylglycerol ether analogs) glycerophospholipids (diphytanylglycerolphosphate ether analogs) Halobacterium salinarum purple membrane matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520423120 |
work_keys_str_mv |
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