In situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ERE-CALUX bioassay
Organic-diffusive gradients in thin-film samplers (o-DGT), were developed and applied for accumulation of estrogen and estrogen-like compounds on a XAD18 resin and deployed in situ in the effluents of Beijing Gaobeidian Wastewater Treatment Plant (GWWTP) and Brussels North Wastewater Treatment Plant...
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doaj-8dc6eb7f72c94cf5abc39d1d424401202020-11-25T02:18:33ZengElsevierEnvironment International0160-41202019-06-011271320In situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ERE-CALUX bioassayWei Guo0Kersten Van Langenhove1Tara Vandermarken2Michael S. Denison3Marc Elskens4Willy Baeyens5Yue Gao6Analytical, Environmental and Geochemistry (AMGC), Vrije Universiteit Brussel (VUB), 1050, Belgium; School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, ChinaAnalytical, Environmental and Geochemistry (AMGC), Vrije Universiteit Brussel (VUB), 1050, BelgiumAnalytical, Environmental and Geochemistry (AMGC), Vrije Universiteit Brussel (VUB), 1050, BelgiumDepartment of Environmental Toxicology, University of California at Davis, Davis, CA 95616, USAAnalytical, Environmental and Geochemistry (AMGC), Vrije Universiteit Brussel (VUB), 1050, BelgiumAnalytical, Environmental and Geochemistry (AMGC), Vrije Universiteit Brussel (VUB), 1050, BelgiumAnalytical, Environmental and Geochemistry (AMGC), Vrije Universiteit Brussel (VUB), 1050, Belgium; Corresponding author.Organic-diffusive gradients in thin-film samplers (o-DGT), were developed and applied for accumulation of estrogen and estrogen-like compounds on a XAD18 resin and deployed in situ in the effluents of Beijing Gaobeidian Wastewater Treatment Plant (GWWTP) and Brussels North Wastewater Treatment Plant as well as in several aquatic systems in Belgium, including the Zenne River, the Belgian Oostende Harbor and the North Sea. Estrogenic compounds accumulate on the XAD18 resin and the estrogenic activity of the resin extract was measured with the Estrogen Responsive Elements-Chemically Activated LUciferase gene eXpression (ERE-CALUX) bioassay. With this result and by applying Fick's diffusion law, it is possible to calculate the estrogenic activity in the aquatic system, if the diffusion boundary layer (DBL) is known or negligible compared to the hydrogel diffusive layer thickness. The DBL thickness in our study varied from 0.010 to 0.023 cm and ignoring the DBL thickness would for instance, underestimate the estrogenic activity by 10–20%. Estrogenic activities in the secondary effluent of GWWTP were the highest (29 ± 4 ng E2-equivalents L−1), while the lowest level was found at the Belgian Oostende Harbor (0.05 ± 0.01 ng E2-equivalents L−1). Comparable estrogenic activities in water samples measured by o-DGT and grab sampling were obtained, confirming that o-DGT can be efficiently used in various aquatic systems. The advantage of our sampling and measuring method is that very low, time averaged estrogenic activities can be determined, with a minimum of sample treatment. The risk of sample contamination is very low as well as the cost of the whole analytical procedure. Keywords: Estrogenic activity, o-DGT, CALUX, DBL, Effluent, Sea waterhttp://www.sciencedirect.com/science/article/pii/S0160412019304234 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Guo Kersten Van Langenhove Tara Vandermarken Michael S. Denison Marc Elskens Willy Baeyens Yue Gao |
spellingShingle |
Wei Guo Kersten Van Langenhove Tara Vandermarken Michael S. Denison Marc Elskens Willy Baeyens Yue Gao In situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ERE-CALUX bioassay Environment International |
author_facet |
Wei Guo Kersten Van Langenhove Tara Vandermarken Michael S. Denison Marc Elskens Willy Baeyens Yue Gao |
author_sort |
Wei Guo |
title |
In situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ERE-CALUX bioassay |
title_short |
In situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ERE-CALUX bioassay |
title_full |
In situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ERE-CALUX bioassay |
title_fullStr |
In situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ERE-CALUX bioassay |
title_full_unstemmed |
In situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ERE-CALUX bioassay |
title_sort |
in situ measurement of estrogenic activity in various aquatic systems using organic diffusive gradients in thin-film coupled with ere-calux bioassay |
publisher |
Elsevier |
series |
Environment International |
issn |
0160-4120 |
publishDate |
2019-06-01 |
description |
Organic-diffusive gradients in thin-film samplers (o-DGT), were developed and applied for accumulation of estrogen and estrogen-like compounds on a XAD18 resin and deployed in situ in the effluents of Beijing Gaobeidian Wastewater Treatment Plant (GWWTP) and Brussels North Wastewater Treatment Plant as well as in several aquatic systems in Belgium, including the Zenne River, the Belgian Oostende Harbor and the North Sea. Estrogenic compounds accumulate on the XAD18 resin and the estrogenic activity of the resin extract was measured with the Estrogen Responsive Elements-Chemically Activated LUciferase gene eXpression (ERE-CALUX) bioassay. With this result and by applying Fick's diffusion law, it is possible to calculate the estrogenic activity in the aquatic system, if the diffusion boundary layer (DBL) is known or negligible compared to the hydrogel diffusive layer thickness. The DBL thickness in our study varied from 0.010 to 0.023 cm and ignoring the DBL thickness would for instance, underestimate the estrogenic activity by 10–20%. Estrogenic activities in the secondary effluent of GWWTP were the highest (29 ± 4 ng E2-equivalents L−1), while the lowest level was found at the Belgian Oostende Harbor (0.05 ± 0.01 ng E2-equivalents L−1). Comparable estrogenic activities in water samples measured by o-DGT and grab sampling were obtained, confirming that o-DGT can be efficiently used in various aquatic systems. The advantage of our sampling and measuring method is that very low, time averaged estrogenic activities can be determined, with a minimum of sample treatment. The risk of sample contamination is very low as well as the cost of the whole analytical procedure. Keywords: Estrogenic activity, o-DGT, CALUX, DBL, Effluent, Sea water |
url |
http://www.sciencedirect.com/science/article/pii/S0160412019304234 |
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