Sesamol Alleviates Airway Hyperresponsiveness and Oxidative Stress in Asthmatic Mice
Sesamol, isolated from sesame seeds<i> (Sesamum </i><i>indicum</i><i>)</i>, was previously shown to have antioxidative, anti-inflammatory, and anti-tumor effects. Sesamol also inhibited lipopolysaccharide (LPS)-induced pulmonary inflammatory response in rats. Howe...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-04-01
|
Series: | Antioxidants |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3921/9/4/295 |
id |
doaj-8dcaecbf5bb844019ea85269a9f29bfc |
---|---|
record_format |
Article |
spelling |
doaj-8dcaecbf5bb844019ea85269a9f29bfc2020-11-25T02:21:58ZengMDPI AGAntioxidants2076-39212020-04-01929529510.3390/antiox9040295Sesamol Alleviates Airway Hyperresponsiveness and Oxidative Stress in Asthmatic MiceChian-Jiun Liou0Ya-Ling Chen1Ming-Chin Yu2Kuo-Wei Yeh3Szu-Chuan Shen4Wen-Chung Huang5Department of Nursing, Division of Basic Medical Sciences, Research Center for Chinese Herbal Medicine, and Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, TaiwanDepartment of Surgery, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33305, TaiwanDivision of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33305, Taiwan.Graduate Program of Nutrition Science, National Taiwan Normal University, 88 Ting-Chow Rd, Sec 4, Taipei 11676, TaiwanDivision of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33305, TaiwanSesamol, isolated from sesame seeds<i> (Sesamum </i><i>indicum</i><i>)</i>, was previously shown to have antioxidative, anti-inflammatory, and anti-tumor effects. Sesamol also inhibited lipopolysaccharide (LPS)-induced pulmonary inflammatory response in rats. However, it remains unclear how sesamol regulates airway inflammation and oxidative stress in asthmatic mice. This study aimed to investigate the efficacy of sesamol on oxidative stress and airway inflammation in asthmatic mice and tracheal epithelial cells. BALB/c mice were sensitized with ovalbumin, and received oral sesamol on days 14 to 27. Furthermore, BEAS-2B human bronchial epithelial cells were treated with sesamol to investigate inflammatory cytokine levels and oxidative responses in vitro. Our results demonstrated that oral sesamol administration significantly suppressed eosinophil infiltration in the lung, airway hyperresponsiveness, and T helper 2 cell-associated (Th2) cytokine expressions in bronchoalveolar lavage fluid and the lungs. Sesamol also significantly increased glutathione expression and reduced malondialdehyde levels in the lungs of asthmatic mice. We also found that sesamol significantly reduced proinflammatory cytokine levels and eotaxin in inflammatory BEAS-2B cells. Moreover, sesamol alleviated reactive oxygen species formation, and suppressed intercellular cell adhesion molecule-1 (ICAM-1) expression, which reduced monocyte cell adherence. We demonstrated that sesamol showed potential as a therapeutic agent for improving asthma.https://www.mdpi.com/2076-3921/9/4/295airway hyperresponsivenessasthmaeosinophil infiltrationoxidative stresssesamol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chian-Jiun Liou Ya-Ling Chen Ming-Chin Yu Kuo-Wei Yeh Szu-Chuan Shen Wen-Chung Huang |
spellingShingle |
Chian-Jiun Liou Ya-Ling Chen Ming-Chin Yu Kuo-Wei Yeh Szu-Chuan Shen Wen-Chung Huang Sesamol Alleviates Airway Hyperresponsiveness and Oxidative Stress in Asthmatic Mice Antioxidants airway hyperresponsiveness asthma eosinophil infiltration oxidative stress sesamol |
author_facet |
Chian-Jiun Liou Ya-Ling Chen Ming-Chin Yu Kuo-Wei Yeh Szu-Chuan Shen Wen-Chung Huang |
author_sort |
Chian-Jiun Liou |
title |
Sesamol Alleviates Airway Hyperresponsiveness and Oxidative Stress in Asthmatic Mice |
title_short |
Sesamol Alleviates Airway Hyperresponsiveness and Oxidative Stress in Asthmatic Mice |
title_full |
Sesamol Alleviates Airway Hyperresponsiveness and Oxidative Stress in Asthmatic Mice |
title_fullStr |
Sesamol Alleviates Airway Hyperresponsiveness and Oxidative Stress in Asthmatic Mice |
title_full_unstemmed |
Sesamol Alleviates Airway Hyperresponsiveness and Oxidative Stress in Asthmatic Mice |
title_sort |
sesamol alleviates airway hyperresponsiveness and oxidative stress in asthmatic mice |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2020-04-01 |
description |
Sesamol, isolated from sesame seeds<i> (Sesamum </i><i>indicum</i><i>)</i>, was previously shown to have antioxidative, anti-inflammatory, and anti-tumor effects. Sesamol also inhibited lipopolysaccharide (LPS)-induced pulmonary inflammatory response in rats. However, it remains unclear how sesamol regulates airway inflammation and oxidative stress in asthmatic mice. This study aimed to investigate the efficacy of sesamol on oxidative stress and airway inflammation in asthmatic mice and tracheal epithelial cells. BALB/c mice were sensitized with ovalbumin, and received oral sesamol on days 14 to 27. Furthermore, BEAS-2B human bronchial epithelial cells were treated with sesamol to investigate inflammatory cytokine levels and oxidative responses in vitro. Our results demonstrated that oral sesamol administration significantly suppressed eosinophil infiltration in the lung, airway hyperresponsiveness, and T helper 2 cell-associated (Th2) cytokine expressions in bronchoalveolar lavage fluid and the lungs. Sesamol also significantly increased glutathione expression and reduced malondialdehyde levels in the lungs of asthmatic mice. We also found that sesamol significantly reduced proinflammatory cytokine levels and eotaxin in inflammatory BEAS-2B cells. Moreover, sesamol alleviated reactive oxygen species formation, and suppressed intercellular cell adhesion molecule-1 (ICAM-1) expression, which reduced monocyte cell adherence. We demonstrated that sesamol showed potential as a therapeutic agent for improving asthma. |
topic |
airway hyperresponsiveness asthma eosinophil infiltration oxidative stress sesamol |
url |
https://www.mdpi.com/2076-3921/9/4/295 |
work_keys_str_mv |
AT chianjiunliou sesamolalleviatesairwayhyperresponsivenessandoxidativestressinasthmaticmice AT yalingchen sesamolalleviatesairwayhyperresponsivenessandoxidativestressinasthmaticmice AT mingchinyu sesamolalleviatesairwayhyperresponsivenessandoxidativestressinasthmaticmice AT kuoweiyeh sesamolalleviatesairwayhyperresponsivenessandoxidativestressinasthmaticmice AT szuchuanshen sesamolalleviatesairwayhyperresponsivenessandoxidativestressinasthmaticmice AT wenchunghuang sesamolalleviatesairwayhyperresponsivenessandoxidativestressinasthmaticmice |
_version_ |
1724864253944397824 |