Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
SUMMARY: Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for...
Main Authors: | Hiroyuki Kojima, Toshimasa Itoh |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-06-01
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Series: | STAR Protocols |
Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672030023X |
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