Fluorescent Submicron-Sized Poly(heptafluoro-<i>n</i>-butyl methacrylate) Particles with Long-Term Stability
Fluorescent submicron particles of fluorinated methacrylate (HFMBA) with long-term stability have been synthesized and characterized with regard to their potential applications. Rhodamine B (RBITC) isothiocyanate was used as the fluorescent component. The core–shell structure of the particles effect...
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doaj-8de6b532514b4bf5b5ff425663e73b012020-11-25T02:34:37ZengMDPI AGMolecules1420-30492020-04-01252013201310.3390/molecules25092013Fluorescent Submicron-Sized Poly(heptafluoro-<i>n</i>-butyl methacrylate) Particles with Long-Term StabilityMaciej Jarzębski0Przemysław Siejak1Monika Przeor2Jacek Gapiński3Anna Woźniak4Hanna Maria Baranowska5Jarosław Pawlicz6Elżbieta Baryła-Pankiewicz7Anna Szwajca8Department of Physics and Biophysics, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 38/42, 60-637 Poznań, PolandDepartment of Physics and Biophysics, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 38/42, 60-637 Poznań, PolandDepartment of Gastronomy Science and Functional Foods, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandMolecular Biophysics Division, Faculty of Physics, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, PolandNanoBioMedical Centre, Adam Mickiewicz University in Poznań, Wszechnicy Piastowskiej 3, 61-614 Poznań, PolandDepartment of Physics and Biophysics, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 38/42, 60-637 Poznań, PolandDepartment of Orthopedics and Traumatology, Poznan University of Medical Sciences, 28 Czerwca 1956 str. No. 135/147, 61-545 Poznań, PolandFaculty of Health Sciences Pomeranian Medical University in Szczecin, Żołnierska 48, 71-210 Szczecin, PolandDepartment of Chemistry, Adam Mickiewicz University in Poznań; Uniwersytetu Poznańskiego 8, 61-614 Poznań, PolandFluorescent submicron particles of fluorinated methacrylate (HFMBA) with long-term stability have been synthesized and characterized with regard to their potential applications. Rhodamine B (RBITC) isothiocyanate was used as the fluorescent component. The core–shell structure of the particles effectively protected the dye against bleaching. HFBMA nanoparticle (NP) stability was confirmed after seven years of storage. Only slight differences were found in the polydispersity index (pdi) from 0.002 to 0.010. Particle size measurements were carried out using dynamic light scattering (DLS), nanoparticle tracking (NTA), and fluorescence correlation spectroscopy (FCS). The hydrodynamic diameter evaluated by different methods were in good agreement, respectively: 184–550 nm, 218–579 nm, and 236–508 nm. Particle and core morphology was estimated by using scanning and transmission electron microscopy (SEM and TEM). The ability to recognize particles in 3D as a reference sample in biological media has been confirmed by epifluorescence optical microscopy, confocal laser scanning microscopy, and super-resolution confocal microscopy (STED).https://www.mdpi.com/1420-3049/25/9/2013fluorinated particlescore–shell NPsRhodamine Bfluorescenceconfocal microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maciej Jarzębski Przemysław Siejak Monika Przeor Jacek Gapiński Anna Woźniak Hanna Maria Baranowska Jarosław Pawlicz Elżbieta Baryła-Pankiewicz Anna Szwajca |
spellingShingle |
Maciej Jarzębski Przemysław Siejak Monika Przeor Jacek Gapiński Anna Woźniak Hanna Maria Baranowska Jarosław Pawlicz Elżbieta Baryła-Pankiewicz Anna Szwajca Fluorescent Submicron-Sized Poly(heptafluoro-<i>n</i>-butyl methacrylate) Particles with Long-Term Stability Molecules fluorinated particles core–shell NPs Rhodamine B fluorescence confocal microscopy |
author_facet |
Maciej Jarzębski Przemysław Siejak Monika Przeor Jacek Gapiński Anna Woźniak Hanna Maria Baranowska Jarosław Pawlicz Elżbieta Baryła-Pankiewicz Anna Szwajca |
author_sort |
Maciej Jarzębski |
title |
Fluorescent Submicron-Sized Poly(heptafluoro-<i>n</i>-butyl methacrylate) Particles with Long-Term Stability |
title_short |
Fluorescent Submicron-Sized Poly(heptafluoro-<i>n</i>-butyl methacrylate) Particles with Long-Term Stability |
title_full |
Fluorescent Submicron-Sized Poly(heptafluoro-<i>n</i>-butyl methacrylate) Particles with Long-Term Stability |
title_fullStr |
Fluorescent Submicron-Sized Poly(heptafluoro-<i>n</i>-butyl methacrylate) Particles with Long-Term Stability |
title_full_unstemmed |
Fluorescent Submicron-Sized Poly(heptafluoro-<i>n</i>-butyl methacrylate) Particles with Long-Term Stability |
title_sort |
fluorescent submicron-sized poly(heptafluoro-<i>n</i>-butyl methacrylate) particles with long-term stability |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-04-01 |
description |
Fluorescent submicron particles of fluorinated methacrylate (HFMBA) with long-term stability have been synthesized and characterized with regard to their potential applications. Rhodamine B (RBITC) isothiocyanate was used as the fluorescent component. The core–shell structure of the particles effectively protected the dye against bleaching. HFBMA nanoparticle (NP) stability was confirmed after seven years of storage. Only slight differences were found in the polydispersity index (pdi) from 0.002 to 0.010. Particle size measurements were carried out using dynamic light scattering (DLS), nanoparticle tracking (NTA), and fluorescence correlation spectroscopy (FCS). The hydrodynamic diameter evaluated by different methods were in good agreement, respectively: 184–550 nm, 218–579 nm, and 236–508 nm. Particle and core morphology was estimated by using scanning and transmission electron microscopy (SEM and TEM). The ability to recognize particles in 3D as a reference sample in biological media has been confirmed by epifluorescence optical microscopy, confocal laser scanning microscopy, and super-resolution confocal microscopy (STED). |
topic |
fluorinated particles core–shell NPs Rhodamine B fluorescence confocal microscopy |
url |
https://www.mdpi.com/1420-3049/25/9/2013 |
work_keys_str_mv |
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