Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive Bacteria
A new haloalkaliphilic species of Wenzhouxiangella, strain AB-CW3, was isolated from a system of hypersaline alkaline soda lakes in the Kulunda Steppe using cells of Staphylococcus aureus as growth substrate. AB-CW3’s complete, circular genome was assembled from combined nanopore and Illumina sequen...
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doaj-8df218cb1eca4e33bc44314a594048362020-11-25T03:59:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.597686597686Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive BacteriaDimitry Y. Sorokin0Dimitry Y. Sorokin1Damon Mosier2Jackie K. Zorz3Xiaoli Dong4Marc Strous5Winogradsky Institute of Microbiology, Federal Research Centre for Biotechnology, Russian Academy of Sciences, Moscow, RussiaDepartment of Biotechnology, Delft University of Technology, Delft, NetherlandsDepartment of Geoscience, University of Calgary, Calgary, AB, CanadaDepartment of Geoscience, University of Calgary, Calgary, AB, CanadaDepartment of Geoscience, University of Calgary, Calgary, AB, CanadaDepartment of Geoscience, University of Calgary, Calgary, AB, CanadaA new haloalkaliphilic species of Wenzhouxiangella, strain AB-CW3, was isolated from a system of hypersaline alkaline soda lakes in the Kulunda Steppe using cells of Staphylococcus aureus as growth substrate. AB-CW3’s complete, circular genome was assembled from combined nanopore and Illumina sequencing and its proteome was determined for three different experimental conditions. AB-CW3 is an aerobic gammaproteobacterium feeding mainly on proteins and peptides. Unique among Wenzhouxiangella, it uses a flagellum for motility, fimbria for cell attachment and is capable of complete denitrification. AB-CW3 can use proteins derived from living or dead cells of Staphylococcus and other Gram-positive bacteria as the carbon and energy source. It encodes and expresses production of a novel Lantibiotic, a class of antimicrobial peptides which have so far only been found to be produced by Gram-positive bacteria. AB-CW3 likely excretes this peptide via a type I secretion system encoded upstream of the genes for production of the Lanthipeptide. Comparison of AB-CW3’s genome to 18 other Wenzhouxiangella genomes from marine, hypersaline, and soda lake habitats indicated one or two transitions from marine to soda lake environments followed by a transition of W. marina back to the oceans. Only 19 genes appear to set haloalkaliphilic Wenzhouxiangella apart from their neutrophilic relatives. As strain AB-CW3 is only distantly related to other members of the genus, we propose to provisionally name it “Wenzhouxiangella alkaliphila”.https://www.frontiersin.org/articles/10.3389/fmicb.2020.597686/fulllantibioticWenzhouxiangelladenitrificationbacteriovoresproteolytic alkaline soda lakenanopore sequencing |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dimitry Y. Sorokin Dimitry Y. Sorokin Damon Mosier Jackie K. Zorz Xiaoli Dong Marc Strous |
spellingShingle |
Dimitry Y. Sorokin Dimitry Y. Sorokin Damon Mosier Jackie K. Zorz Xiaoli Dong Marc Strous Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive Bacteria Frontiers in Microbiology lantibiotic Wenzhouxiangella denitrification bacteriovores proteolytic alkaline soda lake nanopore sequencing |
author_facet |
Dimitry Y. Sorokin Dimitry Y. Sorokin Damon Mosier Jackie K. Zorz Xiaoli Dong Marc Strous |
author_sort |
Dimitry Y. Sorokin |
title |
Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive Bacteria |
title_short |
Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive Bacteria |
title_full |
Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive Bacteria |
title_fullStr |
Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive Bacteria |
title_full_unstemmed |
Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive Bacteria |
title_sort |
wenzhouxiangella strain ab-cw3, a proteolytic bacterium from hypersaline soda lakes that preys on cells of gram-positive bacteria |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-11-01 |
description |
A new haloalkaliphilic species of Wenzhouxiangella, strain AB-CW3, was isolated from a system of hypersaline alkaline soda lakes in the Kulunda Steppe using cells of Staphylococcus aureus as growth substrate. AB-CW3’s complete, circular genome was assembled from combined nanopore and Illumina sequencing and its proteome was determined for three different experimental conditions. AB-CW3 is an aerobic gammaproteobacterium feeding mainly on proteins and peptides. Unique among Wenzhouxiangella, it uses a flagellum for motility, fimbria for cell attachment and is capable of complete denitrification. AB-CW3 can use proteins derived from living or dead cells of Staphylococcus and other Gram-positive bacteria as the carbon and energy source. It encodes and expresses production of a novel Lantibiotic, a class of antimicrobial peptides which have so far only been found to be produced by Gram-positive bacteria. AB-CW3 likely excretes this peptide via a type I secretion system encoded upstream of the genes for production of the Lanthipeptide. Comparison of AB-CW3’s genome to 18 other Wenzhouxiangella genomes from marine, hypersaline, and soda lake habitats indicated one or two transitions from marine to soda lake environments followed by a transition of W. marina back to the oceans. Only 19 genes appear to set haloalkaliphilic Wenzhouxiangella apart from their neutrophilic relatives. As strain AB-CW3 is only distantly related to other members of the genus, we propose to provisionally name it “Wenzhouxiangella alkaliphila”. |
topic |
lantibiotic Wenzhouxiangella denitrification bacteriovores proteolytic alkaline soda lake nanopore sequencing |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2020.597686/full |
work_keys_str_mv |
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