Intratubular Enterococcus faecalis viability assessment following root canal instrumentation with rotary and reciprocating systems via fluorescence microscopy

• Main Authors: Jamileh Ghoddusi, Ehsan Arian, Maryam Golmohammadi, Maryam Gharechahi, Siavash Moushekhian
• Format: Article
• Language: English
• Published: Tabriz University of Medical Sciences 2020-12-01
• Series: Journal of Dental Research, Dental Clinics, Dental Prospects
• Subjects: antimicrobial test; bacterial viability; enterococcus faecalis; instrumentation; protaper gold system; root canal preparation; waveone systems
• Online Access: https://joddd.tbzmed.ac.ir/PDF/joddd-14-214.pdf
• ISSN: 2008-210X; 2008-2118

Background. The present in vitro study aimed to compare the effectiveness of the WaveOne and ProTaper Gold systems in removing the Enterococcus faecalis biofilm. Methods. Thirty-eight mandibular premolars were selected. The root canals were assigned to standard control (canals serially enlarged with ProTaper Gold S1-S2-F1-F2, n=15) and experimental (canals enlarged with Primary WaveOne file, n=15) groups. Following the instrumentation procedure, the root canals underwent a sampling procedure, and the colonyforming unit (CFU) counts were determined. The samples were also evaluated under a fluorescent microscope to evaluate viable bacteria. The data were analyzed using independent samples t test and paired samples t test. Results. The results showed that, compared with the ProTaper group, the WaveOne group exhibited the least viable bacteria (P=0.004). Conclusion. It was concluded that comparison with the ProTaper Gold rotary system, the WaveOne reciprocating file is more successful in reducing intratubular viable bacteria counts.