Quantitative Analysis of Polysaccharide Composition in <i>Polyporus umbellatus</i> by HPLC–ESI–TOF–MS
<i>Polyporus umbellatus</i> is a well-known and important medicinal fungus in Asia. Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects. A qualitative and quantitative method has been established for the anal...
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doaj-8e902efa7cc24a3e882b8fc77c35ed782020-11-25T00:22:24ZengMDPI AGMolecules1420-30492019-07-012414252610.3390/molecules24142526molecules24142526Quantitative Analysis of Polysaccharide Composition in <i>Polyporus umbellatus</i> by HPLC–ESI–TOF–MSNing Guo0Zongli Bai1Weijuan Jia2Jianhua Sun3Wanwan Wang4Shizhong Chen5Hong Wang6School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaKangmei Pharmaceutical Co.Ltd, Puning 515300, ChinaSchool of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaKangmei Pharmaceutical Co.Ltd, Puning 515300, ChinaSchool of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaSchool of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaSchool of Pharmaceutical Sciences, Peking University, Beijing 100191, China<i>Polyporus umbellatus</i> is a well-known and important medicinal fungus in Asia. Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects. A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of <i>Polyporus umbellatus</i> based on high-performance liquid chromatography coupled with electrospray ionization−ion trap−time of flight−mass spectrometry. The hydrolysis conditions of the polysaccharides were optimized by orthogonal design. The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C. The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method. Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides. The results showed that polysaccharides in <i>Polyporus umbellatus</i> were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose. Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10−103.70%. This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of <i>Polyporusumbellatus</i> as a natural medicine.https://www.mdpi.com/1420-3049/24/14/2526composition analysisHPLC–MSmonosaccharide hydrolysis<i>Polyporus umbellatus</i>polysaccharide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ning Guo Zongli Bai Weijuan Jia Jianhua Sun Wanwan Wang Shizhong Chen Hong Wang |
spellingShingle |
Ning Guo Zongli Bai Weijuan Jia Jianhua Sun Wanwan Wang Shizhong Chen Hong Wang Quantitative Analysis of Polysaccharide Composition in <i>Polyporus umbellatus</i> by HPLC–ESI–TOF–MS Molecules composition analysis HPLC–MS monosaccharide hydrolysis <i>Polyporus umbellatus</i> polysaccharide |
author_facet |
Ning Guo Zongli Bai Weijuan Jia Jianhua Sun Wanwan Wang Shizhong Chen Hong Wang |
author_sort |
Ning Guo |
title |
Quantitative Analysis of Polysaccharide Composition in <i>Polyporus umbellatus</i> by HPLC–ESI–TOF–MS |
title_short |
Quantitative Analysis of Polysaccharide Composition in <i>Polyporus umbellatus</i> by HPLC–ESI–TOF–MS |
title_full |
Quantitative Analysis of Polysaccharide Composition in <i>Polyporus umbellatus</i> by HPLC–ESI–TOF–MS |
title_fullStr |
Quantitative Analysis of Polysaccharide Composition in <i>Polyporus umbellatus</i> by HPLC–ESI–TOF–MS |
title_full_unstemmed |
Quantitative Analysis of Polysaccharide Composition in <i>Polyporus umbellatus</i> by HPLC–ESI–TOF–MS |
title_sort |
quantitative analysis of polysaccharide composition in <i>polyporus umbellatus</i> by hplc–esi–tof–ms |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-07-01 |
description |
<i>Polyporus umbellatus</i> is a well-known and important medicinal fungus in Asia. Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects. A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of <i>Polyporus umbellatus</i> based on high-performance liquid chromatography coupled with electrospray ionization−ion trap−time of flight−mass spectrometry. The hydrolysis conditions of the polysaccharides were optimized by orthogonal design. The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C. The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method. Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides. The results showed that polysaccharides in <i>Polyporus umbellatus</i> were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose. Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10−103.70%. This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of <i>Polyporusumbellatus</i> as a natural medicine. |
topic |
composition analysis HPLC–MS monosaccharide hydrolysis <i>Polyporus umbellatus</i> polysaccharide |
url |
https://www.mdpi.com/1420-3049/24/14/2526 |
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