Transgenic Artemisia dubia WALL showed altered phytochemistry and pharmacology
The rol genes have been shown to enhance the production of secondary metabolites in plants. This report examines the effect of trans-genes (rol ABC) on possible high production of biologically important phytochemicals and enhanced pharmacological activities. Three transgenic lines (1, 2 and 3) of Ar...
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doaj-8eb920db43704ce8a7ef08ab555b90c12020-11-25T03:30:34ZengElsevierArabian Journal of Chemistry1878-53522019-12-0112826442654Transgenic Artemisia dubia WALL showed altered phytochemistry and pharmacologyBushra Hafeez Kiani0Nazif Ullah1Ihsan-ul Haq2Bushra Mirza3Department of Biochemistry, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, PakistanDepartment of Pharmacy, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biochemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan; Corresponding author. Tel.: +92 51 90643007.The rol genes have been shown to enhance the production of secondary metabolites in plants. This report examines the effect of trans-genes (rol ABC) on possible high production of biologically important phytochemicals and enhanced pharmacological activities. Three transgenic lines (1, 2 and 3) of Artemisia dubia WALL (transformed with Agrobacterium tumefaciens harboring rol ABC genes) were subjected to phytochemical analysis and pharmacological studies. A great variation in phytochemistry and the pharmacological activities was observed not only between the transgenic and non-transgenic control plants but also among the transgenic lines itself. Comparative chemical profile obtained via HPLC, TLC and spectrophotometry showed high degree of variations in the quantity of phytochemicals. An increased production of total flavonoids (71.1% in transgenic line 2) and total phenolics (110.8% in transgenic line 1), increase in caffeic acid and catechin and a decrease in gallic acid content in the extracts of transformed plants compared to the untransformed control plants was decreased. In case of pharmacological activities, moderate to high level increase in antimicrobial (antibacterial and antifungal) activities, cytotoxicity (14.1%), antitumor (29%) and antioxidant activities (23.9%) was observed (in transgenic line 2). In general all the three transgenic lines under study showed improvement in their pharmacological activities in the order of transgenic line 2 > 1 > 3 > control. The implication of these findings will help to meet the increasing demand of pharmacologically important compounds. Keywords: Artemisia dubiahttp://www.sciencedirect.com/science/article/pii/S1878535215001227 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bushra Hafeez Kiani Nazif Ullah Ihsan-ul Haq Bushra Mirza |
spellingShingle |
Bushra Hafeez Kiani Nazif Ullah Ihsan-ul Haq Bushra Mirza Transgenic Artemisia dubia WALL showed altered phytochemistry and pharmacology Arabian Journal of Chemistry |
author_facet |
Bushra Hafeez Kiani Nazif Ullah Ihsan-ul Haq Bushra Mirza |
author_sort |
Bushra Hafeez Kiani |
title |
Transgenic Artemisia dubia WALL showed altered phytochemistry and pharmacology |
title_short |
Transgenic Artemisia dubia WALL showed altered phytochemistry and pharmacology |
title_full |
Transgenic Artemisia dubia WALL showed altered phytochemistry and pharmacology |
title_fullStr |
Transgenic Artemisia dubia WALL showed altered phytochemistry and pharmacology |
title_full_unstemmed |
Transgenic Artemisia dubia WALL showed altered phytochemistry and pharmacology |
title_sort |
transgenic artemisia dubia wall showed altered phytochemistry and pharmacology |
publisher |
Elsevier |
series |
Arabian Journal of Chemistry |
issn |
1878-5352 |
publishDate |
2019-12-01 |
description |
The rol genes have been shown to enhance the production of secondary metabolites in plants. This report examines the effect of trans-genes (rol ABC) on possible high production of biologically important phytochemicals and enhanced pharmacological activities. Three transgenic lines (1, 2 and 3) of Artemisia dubia WALL (transformed with Agrobacterium tumefaciens harboring rol ABC genes) were subjected to phytochemical analysis and pharmacological studies. A great variation in phytochemistry and the pharmacological activities was observed not only between the transgenic and non-transgenic control plants but also among the transgenic lines itself. Comparative chemical profile obtained via HPLC, TLC and spectrophotometry showed high degree of variations in the quantity of phytochemicals. An increased production of total flavonoids (71.1% in transgenic line 2) and total phenolics (110.8% in transgenic line 1), increase in caffeic acid and catechin and a decrease in gallic acid content in the extracts of transformed plants compared to the untransformed control plants was decreased. In case of pharmacological activities, moderate to high level increase in antimicrobial (antibacterial and antifungal) activities, cytotoxicity (14.1%), antitumor (29%) and antioxidant activities (23.9%) was observed (in transgenic line 2). In general all the three transgenic lines under study showed improvement in their pharmacological activities in the order of transgenic line 2 > 1 > 3 > control. The implication of these findings will help to meet the increasing demand of pharmacologically important compounds. Keywords: Artemisia dubia |
url |
http://www.sciencedirect.com/science/article/pii/S1878535215001227 |
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