Genome-wide identification and expression of GRAS gene family members in cassava
Abstract Background Cassava is highly tolerant to stressful conditions, especially drought stress conditions; however, the mechanisms underlying this tolerance are poorly understood. The GRAS gene family is a large family of transcription factors that are involved in regulating the growth, developme...
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doaj-8ec352cf94414d80a7b26556f4ea5efc2021-01-31T16:06:23ZengBMCBMC Plant Biology1471-22292020-01-0120111610.1186/s12870-020-2242-8Genome-wide identification and expression of GRAS gene family members in cassavaZhongying Shan0Xinglu Luo1Meiyan Wu2Limei Wei3Zhupeng Fan4Yanmei Zhu5Agricultural College, Guangxi UniversityAgricultural College, Guangxi UniversityAgricultural College, Guangxi UniversityAgricultural College, Guangxi UniversityAgricultural College, Guangxi UniversityAgricultural College, Guangxi UniversityAbstract Background Cassava is highly tolerant to stressful conditions, especially drought stress conditions; however, the mechanisms underlying this tolerance are poorly understood. The GRAS gene family is a large family of transcription factors that are involved in regulating the growth, development, and stress responses of plants. Currently, GRAS transcription factors have not been systematically studied in cassava, which is the sixth most important crop in the world. Results Seventy-seven MeGRAS genes were identified from the cassava genome database. Phylogenetic analysis revealed that the MeGRAS proteins could be divided into 14 subfamilies. The gene structure and motif compositions of the proteins were considerably conserved within the same subfamily. Duplication events, particularly segmental duplication, were identified as the main driving force for GRAS gene expansion in cassava. Global expression analysis revealed that MeGRAS genes exhibited similar or distinct expression profiles within different tissues among different varieties. Moreover, qRT-PCR analysis revealed the expression patterns of MeGRAS genes in response to abiotic stress (drought, salt, cold, and H2O2), and the results suggest that these genes may have multiple functions. Conclusion This study is the first to provide comprehensive information on GRAS gene family members in cassava. The data will increase our understanding of both the molecular basis and the effects of GRAS genes. In addition, the results will contribute further to identifying the responses to various environmental conditions and provide insights into the potential functions of GRAS genes.https://doi.org/10.1186/s12870-020-2242-8CassavaGRAS genesGene expressionAbiotic stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhongying Shan Xinglu Luo Meiyan Wu Limei Wei Zhupeng Fan Yanmei Zhu |
spellingShingle |
Zhongying Shan Xinglu Luo Meiyan Wu Limei Wei Zhupeng Fan Yanmei Zhu Genome-wide identification and expression of GRAS gene family members in cassava BMC Plant Biology Cassava GRAS genes Gene expression Abiotic stress |
author_facet |
Zhongying Shan Xinglu Luo Meiyan Wu Limei Wei Zhupeng Fan Yanmei Zhu |
author_sort |
Zhongying Shan |
title |
Genome-wide identification and expression of GRAS gene family members in cassava |
title_short |
Genome-wide identification and expression of GRAS gene family members in cassava |
title_full |
Genome-wide identification and expression of GRAS gene family members in cassava |
title_fullStr |
Genome-wide identification and expression of GRAS gene family members in cassava |
title_full_unstemmed |
Genome-wide identification and expression of GRAS gene family members in cassava |
title_sort |
genome-wide identification and expression of gras gene family members in cassava |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2020-01-01 |
description |
Abstract Background Cassava is highly tolerant to stressful conditions, especially drought stress conditions; however, the mechanisms underlying this tolerance are poorly understood. The GRAS gene family is a large family of transcription factors that are involved in regulating the growth, development, and stress responses of plants. Currently, GRAS transcription factors have not been systematically studied in cassava, which is the sixth most important crop in the world. Results Seventy-seven MeGRAS genes were identified from the cassava genome database. Phylogenetic analysis revealed that the MeGRAS proteins could be divided into 14 subfamilies. The gene structure and motif compositions of the proteins were considerably conserved within the same subfamily. Duplication events, particularly segmental duplication, were identified as the main driving force for GRAS gene expansion in cassava. Global expression analysis revealed that MeGRAS genes exhibited similar or distinct expression profiles within different tissues among different varieties. Moreover, qRT-PCR analysis revealed the expression patterns of MeGRAS genes in response to abiotic stress (drought, salt, cold, and H2O2), and the results suggest that these genes may have multiple functions. Conclusion This study is the first to provide comprehensive information on GRAS gene family members in cassava. The data will increase our understanding of both the molecular basis and the effects of GRAS genes. In addition, the results will contribute further to identifying the responses to various environmental conditions and provide insights into the potential functions of GRAS genes. |
topic |
Cassava GRAS genes Gene expression Abiotic stress |
url |
https://doi.org/10.1186/s12870-020-2242-8 |
work_keys_str_mv |
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