Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine model

Background and study aims Endoscopic ultrasound (EUS) has been used for portal vein sampling in patients with pancreaticobiliary cancers for enumerating circulating tumor cells but is not yet a standard procedure. Further evaluation is needed to refine the methodology. Therefore, we evaluated the fe...

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Main Authors: Kenneth Park, Daniel Lew, Christopher Chapman, Ashley Wachsman, Matthew Bloom, Liiana Bancila, Rachel Perry, Qiang Wang, Laith Jamil, Stephen Pandol, Simon Lo
Format: Article
Language:English
Published: Georg Thieme Verlag KG 2020-10-01
Series:Endoscopy International Open
Online Access:http://www.thieme-connect.de/DOI/DOI?10.1055/a-1264-7206
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spelling doaj-8ec75c22bf404d1fae179ec4ac31afbc2020-11-25T03:52:06ZengGeorg Thieme Verlag KGEndoscopy International Open2364-37222196-97362020-10-010811E1717E172410.1055/a-1264-7206Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine modelKenneth Park0Daniel Lew1Christopher Chapman2Ashley Wachsman3Matthew Bloom4Liiana Bancila5Rachel Perry6Qiang Wang7Laith Jamil8Stephen Pandol9Simon Lo10Cedars-Sinai Medical Center, Division of Digestive Diseases, Los Angeles, CaliforniaCedars-Sinai Medical Center, Division of Digestive Diseases, Los Angeles, CaliforniaUniversity of Chicago Medical Center, Center for Endoscopic Research and Therapeutics, Chicago, Illinois, United StatesCedars-Sinai Medical Center, Division of Digestive Diseases, Los Angeles, CaliforniaCedars-Sinai Medical Center – Surgery, Los Angeles, California, United StatesCedars-Sinai Medical Center, Division of Digestive Diseases, Los Angeles, CaliforniaCedars-Sinai Medical Center, Division of Digestive Diseases, Los Angeles, CaliforniaCedars-Sinai Medical Center, Division of Digestive Diseases, Los Angeles, CaliforniaWilliam Beaumont Hospital – Royal Oak, Gastroenterology and Hepatology, Royal Oak, Michigan, United StatesCedars-Sinai Medical Center, Division of Digestive Diseases, Los Angeles, CaliforniaCedars-Sinai Medical Center, Division of Digestive Diseases, Los Angeles, CaliforniaBackground and study aims Endoscopic ultrasound (EUS) has been used for portal vein sampling in patients with pancreaticobiliary cancers for enumerating circulating tumor cells but is not yet a standard procedure. Further evaluation is needed to refine the methodology. Therefore, we evaluated the feasibility and safety of 19-gauge (19G) versus a 22-gauge (22 G) EUS fine-needle aspiration needles for portal vein sampling in a swine model. Methods Celiotomy was performed on two farm pigs. Portal vein sampling occurred transhepatically. We compared 19 G and 22 G needles coated interiorly with saline, heparin or ethylenediaminetetraacetic acid (EDTA). Small- (10 mL) and large- (25 mL) volume blood collections were evaluated. Two different collection methods were tested: direct-to-vial and suction syringe. A bleeding risk trial for saline-coated 19 G and 22 G needles was performed by puncturing the portal vein 20 times. Persistent bleeding after 3 minutes was considered significant. Results All small-volume collection trials were successful except for 22 G saline-coated needles with direct-to-vial method. All large-volume collection trials were successful when using suction syringe; direct-to-vial method for both 19 G and 22 G needles were unsuccessful. Collection times were shorter for 19 G vs. 22 G needles for both small and large-volume collections (P < 0.05). Collection times for saline-coated 22 G needles were longer compared to heparin/EDTA-coated (P < 0.05). Bleeding occurred in 10 % punctures with 19 G needles compared to 0 % with 22 G needles. Conclusion The results of this animal study demonstrate the feasibility and the safety of using 22 G needles for portal vein sampling and can form the basis for a pilot study in patients.http://www.thieme-connect.de/DOI/DOI?10.1055/a-1264-7206
collection DOAJ
language English
format Article
sources DOAJ
author Kenneth Park
Daniel Lew
Christopher Chapman
Ashley Wachsman
Matthew Bloom
Liiana Bancila
Rachel Perry
Qiang Wang
Laith Jamil
Stephen Pandol
Simon Lo
spellingShingle Kenneth Park
Daniel Lew
Christopher Chapman
Ashley Wachsman
Matthew Bloom
Liiana Bancila
Rachel Perry
Qiang Wang
Laith Jamil
Stephen Pandol
Simon Lo
Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine model
Endoscopy International Open
author_facet Kenneth Park
Daniel Lew
Christopher Chapman
Ashley Wachsman
Matthew Bloom
Liiana Bancila
Rachel Perry
Qiang Wang
Laith Jamil
Stephen Pandol
Simon Lo
author_sort Kenneth Park
title Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine model
title_short Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine model
title_full Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine model
title_fullStr Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine model
title_full_unstemmed Feasibility and safety study of 22-gauge endoscopic ultrasound (EUS) needles for portal vein sampling in a swine model
title_sort feasibility and safety study of 22-gauge endoscopic ultrasound (eus) needles for portal vein sampling in a swine model
publisher Georg Thieme Verlag KG
series Endoscopy International Open
issn 2364-3722
2196-9736
publishDate 2020-10-01
description Background and study aims Endoscopic ultrasound (EUS) has been used for portal vein sampling in patients with pancreaticobiliary cancers for enumerating circulating tumor cells but is not yet a standard procedure. Further evaluation is needed to refine the methodology. Therefore, we evaluated the feasibility and safety of 19-gauge (19G) versus a 22-gauge (22 G) EUS fine-needle aspiration needles for portal vein sampling in a swine model. Methods Celiotomy was performed on two farm pigs. Portal vein sampling occurred transhepatically. We compared 19 G and 22 G needles coated interiorly with saline, heparin or ethylenediaminetetraacetic acid (EDTA). Small- (10 mL) and large- (25 mL) volume blood collections were evaluated. Two different collection methods were tested: direct-to-vial and suction syringe. A bleeding risk trial for saline-coated 19 G and 22 G needles was performed by puncturing the portal vein 20 times. Persistent bleeding after 3 minutes was considered significant. Results All small-volume collection trials were successful except for 22 G saline-coated needles with direct-to-vial method. All large-volume collection trials were successful when using suction syringe; direct-to-vial method for both 19 G and 22 G needles were unsuccessful. Collection times were shorter for 19 G vs. 22 G needles for both small and large-volume collections (P < 0.05). Collection times for saline-coated 22 G needles were longer compared to heparin/EDTA-coated (P < 0.05). Bleeding occurred in 10 % punctures with 19 G needles compared to 0 % with 22 G needles. Conclusion The results of this animal study demonstrate the feasibility and the safety of using 22 G needles for portal vein sampling and can form the basis for a pilot study in patients.
url http://www.thieme-connect.de/DOI/DOI?10.1055/a-1264-7206
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