Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila
The use of natural products as an antimicrobial agent is an alternative way in the treating drug-resistant pathogens. A. hydrophila and V. harveyi are pathogenic bacteria both in freshwater and marine culture, respectively. Physic nut (Jatropha curcas, Linn) has been known as an herbal plant that ha...
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doaj-8ed5d0f3c9ad46959b2e8ec6c2478ffc2020-11-24T21:36:40ZindUniversitas Gadjah MadaJurnal Perikanan Universitas Gadjah Mada0853-63842502-50662017-03-01191111610.22146/jfs.1074119783Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophilaNasrullah Bai Arifin0Imas Marthapratama1Ellana Sanoesi2Arief Prajitno3Program Studi Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas BrawijayaProgram Studi Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas BrawijayaProgram Studi Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya2Laboratorium Parasit dan Penyakit ikan, Fakultas Perikanan dan Ilmu Kelautan, Universitas BrawijayaThe use of natural products as an antimicrobial agent is an alternative way in the treating drug-resistant pathogens. A. hydrophila and V. harveyi are pathogenic bacteria both in freshwater and marine culture, respectively. Physic nut (Jatropha curcas, Linn) has been known as an herbal plant that has an antibacterial agent. The purpose of this study was to analyze inhibition ability of an extract of physic nut leaves on the growth of V. harveyi and A. hydrophila. In this study, water extract of physic nut leaves was determined to evaluate the antibacterial activity against V. harveyi and A. hydrophila using the Minimum Inhibitory Concentration Test (MIC) and Paper Disk Diffusion Method. The smallest concentration on the MIC was 0.10 g/ml for A. hydrophila and 0.15 g/ml for V. harveyi, respectively. The Paper Disk Diffusion Method used difference concentrations (0.10 g/ml; 0.15 g/ml; 0.20 g/ml; 0.25 g/ml; 0.30 g/ml; 0.35 g/ml; dan 0.40 g/ml) and Control (0 g/ml). The concentration of 0.35 g/ml had the highest inhibition zone diameter in 11.3±0.2 mm for A. hydrophila and 0.40 g/ml had the highest inhibition zone in 11.2±0.3 mm for V. harveyi. The result showed that water extract of physic nut leaves was able to inhibit both tested bacteria.https://journal.ugm.ac.id/jfs/article/view/10741Aeromonas hydrophilapaper disk diffusionJatropha curcas Linnminimum inhibitory concentration testVibrio harveyi |
collection |
DOAJ |
language |
Indonesian |
format |
Article |
sources |
DOAJ |
author |
Nasrullah Bai Arifin Imas Marthapratama Ellana Sanoesi Arief Prajitno |
spellingShingle |
Nasrullah Bai Arifin Imas Marthapratama Ellana Sanoesi Arief Prajitno Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila Jurnal Perikanan Universitas Gadjah Mada Aeromonas hydrophila paper disk diffusion Jatropha curcas Linn minimum inhibitory concentration test Vibrio harveyi |
author_facet |
Nasrullah Bai Arifin Imas Marthapratama Ellana Sanoesi Arief Prajitno |
author_sort |
Nasrullah Bai Arifin |
title |
Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila |
title_short |
Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila |
title_full |
Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila |
title_fullStr |
Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila |
title_full_unstemmed |
Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila |
title_sort |
antibacterial activity of jatropha curcas (linn) leaves extract a gainst vibrio harveyi and aeromonas hydrophila |
publisher |
Universitas Gadjah Mada |
series |
Jurnal Perikanan Universitas Gadjah Mada |
issn |
0853-6384 2502-5066 |
publishDate |
2017-03-01 |
description |
The use of natural products as an antimicrobial agent is an alternative way in the treating drug-resistant pathogens. A. hydrophila and V. harveyi are pathogenic bacteria both in freshwater and marine culture, respectively. Physic nut (Jatropha curcas, Linn) has been known as an herbal plant that has an antibacterial agent. The purpose of this study was to analyze inhibition ability of an extract of physic nut leaves on the growth of V. harveyi and A. hydrophila. In this study, water extract of physic nut leaves was determined to evaluate the antibacterial activity against V. harveyi and A. hydrophila using the Minimum Inhibitory Concentration Test (MIC) and Paper Disk Diffusion Method. The smallest concentration on the MIC was 0.10 g/ml for A. hydrophila and 0.15 g/ml for V. harveyi, respectively. The Paper Disk Diffusion Method used difference concentrations (0.10 g/ml; 0.15 g/ml; 0.20 g/ml; 0.25 g/ml; 0.30 g/ml; 0.35 g/ml; dan 0.40 g/ml) and Control (0 g/ml). The concentration of 0.35 g/ml had the highest inhibition zone diameter in 11.3±0.2 mm for A. hydrophila and 0.40 g/ml had the highest inhibition zone in 11.2±0.3 mm for V. harveyi. The result showed that water extract of physic nut leaves was able to inhibit both tested bacteria. |
topic |
Aeromonas hydrophila paper disk diffusion Jatropha curcas Linn minimum inhibitory concentration test Vibrio harveyi |
url |
https://journal.ugm.ac.id/jfs/article/view/10741 |
work_keys_str_mv |
AT nasrullahbaiarifin antibacterialactivityofjatrophacurcaslinnleavesextractagainstvibrioharveyiandaeromonashydrophila AT imasmarthapratama antibacterialactivityofjatrophacurcaslinnleavesextractagainstvibrioharveyiandaeromonashydrophila AT ellanasanoesi antibacterialactivityofjatrophacurcaslinnleavesextractagainstvibrioharveyiandaeromonashydrophila AT ariefprajitno antibacterialactivityofjatrophacurcaslinnleavesextractagainstvibrioharveyiandaeromonashydrophila |
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