Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1q
We addressed the issue of C1q autoantigenicity by studying the structural features of the autoepitopes recognized by the polyclonal anti-C1q antibodies present in Lupus Nephritis (LN) sera. We used six fractions of anti-C1q as antigens and selected anti-idiotypic scFv antibodies from the phage libra...
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doaj-8ef43c4fa65f4c19891cc0c03433f0412021-08-06T15:26:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-01228288828810.3390/ijms22158288Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1qNadezhda Todorova0Miroslav Rangelov1Vanya Bogoeva2Vishnya Stoyanova3Anna Yordanova4Ginka Nikolova5Hristo Georgiev6Daniela Dimitrova7Safa Mohedin8Katerina Stoyanova9Ivanka Tsacheva10Institute of Biodiversity and Ecosystem Research (IBER), Bulgarian Academy of Sciences, Yurii Gagarin Str. Bl. 2, 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry (IOCCP), Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 9, 1113 Sofia, BulgariaInstitute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 21, 1113 Sofia, BulgariaDepartment of Chemistry, Biochemistry, Physiology and Pathophysiology, Medical Faculty of Sofia University, 1 Kozyak Str., 1407 Sofia, BulgariaInstitute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 21, 1113 Sofia, BulgariaDepartment of Biochemistry, Faculty of Biology, Sofia University, 8 D. Tsankov Str., 1164 Sofia, BulgariaInstitute of Immunology, Hannover Medical School, Carl-Neuberg Str. Bl. 1, 30625 Hannover, GermanyInstitute of Biophysics and Biomedical Engineering, Department of Electroinduced and Adhesive Properties, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bl. 21, 1113 Sofia, BulgariaDepartment of Biochemistry, Faculty of Biology, Sofia University, 8 D. Tsankov Str., 1164 Sofia, BulgariaDepartment of Biochemistry, Faculty of Biology, Sofia University, 8 D. Tsankov Str., 1164 Sofia, BulgariaDepartment of Biochemistry, Faculty of Biology, Sofia University, 8 D. Tsankov Str., 1164 Sofia, BulgariaWe addressed the issue of C1q autoantigenicity by studying the structural features of the autoepitopes recognized by the polyclonal anti-C1q antibodies present in Lupus Nephritis (LN) sera. We used six fractions of anti-C1q as antigens and selected anti-idiotypic scFv antibodies from the phage library “Griffin.1”. The monoclonal scFv A1 was the most potent inhibitor of the recognition of C1q and its fragments ghA, ghB and ghC, comprising the globular domain gC1q, by the lupus autoantibodies. It was sequenced and in silico folded by molecular dynamics into a 3D structure. The generated 3D model of A1 elucidated CDR similarity to the apical region of gC1q, thus mapping indirectly for the first time a globular autoepitope of C1q. The V<sub>H</sub> CDR2 of A1 mimicked the ghA sequence GSEAD suggested as a cross-epitope between anti-DNA and anti-C1q antibodies. Other potential inhibitors of the recognition of C1q by the LN autoantibodies among the selected recombinant antibodies were the monoclonal scFv F6, F9 and A12.https://www.mdpi.com/1422-0067/22/15/8288C1qglobular autoepitopesscFvanti-DNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nadezhda Todorova Miroslav Rangelov Vanya Bogoeva Vishnya Stoyanova Anna Yordanova Ginka Nikolova Hristo Georgiev Daniela Dimitrova Safa Mohedin Katerina Stoyanova Ivanka Tsacheva |
spellingShingle |
Nadezhda Todorova Miroslav Rangelov Vanya Bogoeva Vishnya Stoyanova Anna Yordanova Ginka Nikolova Hristo Georgiev Daniela Dimitrova Safa Mohedin Katerina Stoyanova Ivanka Tsacheva Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1q International Journal of Molecular Sciences C1q globular autoepitopes scFv anti-DNA |
author_facet |
Nadezhda Todorova Miroslav Rangelov Vanya Bogoeva Vishnya Stoyanova Anna Yordanova Ginka Nikolova Hristo Georgiev Daniela Dimitrova Safa Mohedin Katerina Stoyanova Ivanka Tsacheva |
author_sort |
Nadezhda Todorova |
title |
Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1q |
title_short |
Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1q |
title_full |
Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1q |
title_fullStr |
Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1q |
title_full_unstemmed |
Anti-Idiotype scFv Localizes an Autoepitope in the Globular Domain of C1q |
title_sort |
anti-idiotype scfv localizes an autoepitope in the globular domain of c1q |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-08-01 |
description |
We addressed the issue of C1q autoantigenicity by studying the structural features of the autoepitopes recognized by the polyclonal anti-C1q antibodies present in Lupus Nephritis (LN) sera. We used six fractions of anti-C1q as antigens and selected anti-idiotypic scFv antibodies from the phage library “Griffin.1”. The monoclonal scFv A1 was the most potent inhibitor of the recognition of C1q and its fragments ghA, ghB and ghC, comprising the globular domain gC1q, by the lupus autoantibodies. It was sequenced and in silico folded by molecular dynamics into a 3D structure. The generated 3D model of A1 elucidated CDR similarity to the apical region of gC1q, thus mapping indirectly for the first time a globular autoepitope of C1q. The V<sub>H</sub> CDR2 of A1 mimicked the ghA sequence GSEAD suggested as a cross-epitope between anti-DNA and anti-C1q antibodies. Other potential inhibitors of the recognition of C1q by the LN autoantibodies among the selected recombinant antibodies were the monoclonal scFv F6, F9 and A12. |
topic |
C1q globular autoepitopes scFv anti-DNA |
url |
https://www.mdpi.com/1422-0067/22/15/8288 |
work_keys_str_mv |
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