Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro

• Main Authors: Sonam Chopra, Paulius Ruzgys, Martynas Maciulevičius, Milda Jakutavičiūtė, Saulius Šatkauskas
• Format: Article
• Language: English
• Published: MDPI AG 2020-09-01
• Series: Applied Sciences
• Subjects: plasmid; DNA; electroporation; electrotransfection; cell viability
• Online Access: https://www.mdpi.com/2076-3417/10/17/6070

Electroporation is an effective method for delivering plasmid DNA molecules into cells. The efficiency of gene electrotransfer depends on several factors. To achieve high transfection efficiency while maintaining cell viability is a tedious task in electroporation. Here, we present a combined study in which the dynamics of both evaluation types of transfection efficiency and the cell viability were evaluated in dependence of plasmid concentration as well as at the different number of high voltage (HV) electric pulses. The results of this study reveal a quantitative sigmoidal (R² > 0.95) dependence of the transfection efficiency and cell viability on the distance between the cell membrane and the nearest plasmid. We propose this distance value as a new, more accurate output parameter that could be used in further optimization studies as a predictor and a measure of electrotransfection efficiency.