Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro
Electroporation is an effective method for delivering plasmid DNA molecules into cells. The efficiency of gene electrotransfer depends on several factors. To achieve high transfection efficiency while maintaining cell viability is a tedious task in electroporation. Here, we present a combined study...
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doaj-8f1a691f5a054149a9e89bf4ac6b5ce82020-11-25T02:45:45ZengMDPI AGApplied Sciences2076-34172020-09-01106070607010.3390/app10176070Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In VitroSonam Chopra0Paulius Ruzgys1Martynas Maciulevičius2Milda Jakutavičiūtė3Saulius Šatkauskas4Biophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos Str. 8, LT 44404 Kaunas, LithuaniaBiophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos Str. 8, LT 44404 Kaunas, LithuaniaBiophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos Str. 8, LT 44404 Kaunas, LithuaniaBiophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos Str. 8, LT 44404 Kaunas, LithuaniaBiophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos Str. 8, LT 44404 Kaunas, LithuaniaElectroporation is an effective method for delivering plasmid DNA molecules into cells. The efficiency of gene electrotransfer depends on several factors. To achieve high transfection efficiency while maintaining cell viability is a tedious task in electroporation. Here, we present a combined study in which the dynamics of both evaluation types of transfection efficiency and the cell viability were evaluated in dependence of plasmid concentration as well as at the different number of high voltage (HV) electric pulses. The results of this study reveal a quantitative sigmoidal (R<sup>2</sup> > 0.95) dependence of the transfection efficiency and cell viability on the distance between the cell membrane and the nearest plasmid. We propose this distance value as a new, more accurate output parameter that could be used in further optimization studies as a predictor and a measure of electrotransfection efficiency.https://www.mdpi.com/2076-3417/10/17/6070plasmidDNAelectroporationelectrotransfectioncell viability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sonam Chopra Paulius Ruzgys Martynas Maciulevičius Milda Jakutavičiūtė Saulius Šatkauskas |
spellingShingle |
Sonam Chopra Paulius Ruzgys Martynas Maciulevičius Milda Jakutavičiūtė Saulius Šatkauskas Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro Applied Sciences plasmid DNA electroporation electrotransfection cell viability |
author_facet |
Sonam Chopra Paulius Ruzgys Martynas Maciulevičius Milda Jakutavičiūtė Saulius Šatkauskas |
author_sort |
Sonam Chopra |
title |
Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro |
title_short |
Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro |
title_full |
Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro |
title_fullStr |
Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro |
title_full_unstemmed |
Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro |
title_sort |
investigation of plasmid dna delivery and cell viability dynamics for optimal cell electrotransfection in vitro |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2020-09-01 |
description |
Electroporation is an effective method for delivering plasmid DNA molecules into cells. The efficiency of gene electrotransfer depends on several factors. To achieve high transfection efficiency while maintaining cell viability is a tedious task in electroporation. Here, we present a combined study in which the dynamics of both evaluation types of transfection efficiency and the cell viability were evaluated in dependence of plasmid concentration as well as at the different number of high voltage (HV) electric pulses. The results of this study reveal a quantitative sigmoidal (R<sup>2</sup> > 0.95) dependence of the transfection efficiency and cell viability on the distance between the cell membrane and the nearest plasmid. We propose this distance value as a new, more accurate output parameter that could be used in further optimization studies as a predictor and a measure of electrotransfection efficiency. |
topic |
plasmid DNA electroporation electrotransfection cell viability |
url |
https://www.mdpi.com/2076-3417/10/17/6070 |
work_keys_str_mv |
AT sonamchopra investigationofplasmiddnadeliveryandcellviabilitydynamicsforoptimalcellelectrotransfectioninvitro AT pauliusruzgys investigationofplasmiddnadeliveryandcellviabilitydynamicsforoptimalcellelectrotransfectioninvitro AT martynasmaciulevicius investigationofplasmiddnadeliveryandcellviabilitydynamicsforoptimalcellelectrotransfectioninvitro AT mildajakutaviciute investigationofplasmiddnadeliveryandcellviabilitydynamicsforoptimalcellelectrotransfectioninvitro AT sauliussatkauskas investigationofplasmiddnadeliveryandcellviabilitydynamicsforoptimalcellelectrotransfectioninvitro |
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1724760520863514624 |