Mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice
Abstract Background The Kampo medicine bofutsushosan (BTS; Pulvis ledebouriellae compositae; Fang Feng Tong Sheng San) has been used as an anti-obesity treatment in overweight patients. In this study, we assessed the underlying physiological changes induced by BTS in obese mice maintained on a high-...
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doaj-8f3bb2411378468ca8667a0ebf8f84c22020-11-24T21:55:34ZengBMCChinese Medicine1749-85462017-03-0112111110.1186/s13020-017-0129-xMechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese miceShinjiro Kobayashi0Yuki Kawasaki1Tatsuo Takahashi2Hironori Maeno3Masaaki Nomura4Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Hokuriku UniversityDepartment of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Hokuriku UniversityDepartment of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Hokuriku UniversityDepartment of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Hokuriku UniversityCenter of Clinical Pharmacy Education, Faculty of Pharmaceutical Sciences, Hokuriku UniversityAbstract Background The Kampo medicine bofutsushosan (BTS; Pulvis ledebouriellae compositae; Fang Feng Tong Sheng San) has been used as an anti-obesity treatment in overweight patients. In this study, we assessed the underlying physiological changes induced by BTS in obese mice maintained on a high-fat diet. Methods Male ICR mice were fed a 60% kcal fat diet for 5 weeks starting at 4 weeks of age and then fed the same diet with administration of water (control) or aqueous BTS extract (1.0–2.0 g/kg) for 25 days. Body weight, wet weight of isolated white adipose tissue, and obesity-related serum parameters (glucose, lipids, leptin, adiponectin) were measured after treatment. The mRNA expression levels of leptin, adiponectin, and UCP1 in the adipose tissues were determined by quantitative real-time polymerase chain reaction after the first 5 days of treatment. Results Bofutsushosan (1.5–2.0 g/kg) significantly decreased total body weight and total wet weight of white adipose tissue isolated from subcutaneous (retroperitoneal) and visceral regions (epididymal, mesenteric, and perirenal). At 2.0 g/kg, BTS also decreased total fat mass, visceral fat mass, and ratio of fat mass to body weight as measured by computed tomography, and significantly decreased epididymal adipocyte size after 14 and 25 days’ treatment. Twenty-five days’ treatment lowered serum glucose, insulin, leptin, and triglycerides, and reduced homeostasis model assessment-insulin resistance. Alternatively, 2.0 g/kg BTS significantly increased mRNA levels of adiponectin, leptin, and UCP1 in interscapular brown adipose tissue but not epididymal white adipose tissue after 5 days’ administration. Conclusion In the early administration period, BTS increased mRNA expression levels of leptin, adiponectin, and UCP1 in brown adipose tissues. With longer administration, BTS improved insulin resistance, and subsequently reduced serum levels of leptin and triglyceride in parallel with decreased visceral white adipose tissue volume and adipocyte size.http://link.springer.com/article/10.1186/s13020-017-0129-xBofutsushosan (BTS)High-fat diet-fed obese miceAdiponectin mRNALeptin mRNAUCP1 mRNAInsulin resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shinjiro Kobayashi Yuki Kawasaki Tatsuo Takahashi Hironori Maeno Masaaki Nomura |
spellingShingle |
Shinjiro Kobayashi Yuki Kawasaki Tatsuo Takahashi Hironori Maeno Masaaki Nomura Mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice Chinese Medicine Bofutsushosan (BTS) High-fat diet-fed obese mice Adiponectin mRNA Leptin mRNA UCP1 mRNA Insulin resistance |
author_facet |
Shinjiro Kobayashi Yuki Kawasaki Tatsuo Takahashi Hironori Maeno Masaaki Nomura |
author_sort |
Shinjiro Kobayashi |
title |
Mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice |
title_short |
Mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice |
title_full |
Mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice |
title_fullStr |
Mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice |
title_full_unstemmed |
Mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice |
title_sort |
mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice |
publisher |
BMC |
series |
Chinese Medicine |
issn |
1749-8546 |
publishDate |
2017-03-01 |
description |
Abstract Background The Kampo medicine bofutsushosan (BTS; Pulvis ledebouriellae compositae; Fang Feng Tong Sheng San) has been used as an anti-obesity treatment in overweight patients. In this study, we assessed the underlying physiological changes induced by BTS in obese mice maintained on a high-fat diet. Methods Male ICR mice were fed a 60% kcal fat diet for 5 weeks starting at 4 weeks of age and then fed the same diet with administration of water (control) or aqueous BTS extract (1.0–2.0 g/kg) for 25 days. Body weight, wet weight of isolated white adipose tissue, and obesity-related serum parameters (glucose, lipids, leptin, adiponectin) were measured after treatment. The mRNA expression levels of leptin, adiponectin, and UCP1 in the adipose tissues were determined by quantitative real-time polymerase chain reaction after the first 5 days of treatment. Results Bofutsushosan (1.5–2.0 g/kg) significantly decreased total body weight and total wet weight of white adipose tissue isolated from subcutaneous (retroperitoneal) and visceral regions (epididymal, mesenteric, and perirenal). At 2.0 g/kg, BTS also decreased total fat mass, visceral fat mass, and ratio of fat mass to body weight as measured by computed tomography, and significantly decreased epididymal adipocyte size after 14 and 25 days’ treatment. Twenty-five days’ treatment lowered serum glucose, insulin, leptin, and triglycerides, and reduced homeostasis model assessment-insulin resistance. Alternatively, 2.0 g/kg BTS significantly increased mRNA levels of adiponectin, leptin, and UCP1 in interscapular brown adipose tissue but not epididymal white adipose tissue after 5 days’ administration. Conclusion In the early administration period, BTS increased mRNA expression levels of leptin, adiponectin, and UCP1 in brown adipose tissues. With longer administration, BTS improved insulin resistance, and subsequently reduced serum levels of leptin and triglyceride in parallel with decreased visceral white adipose tissue volume and adipocyte size. |
topic |
Bofutsushosan (BTS) High-fat diet-fed obese mice Adiponectin mRNA Leptin mRNA UCP1 mRNA Insulin resistance |
url |
http://link.springer.com/article/10.1186/s13020-017-0129-x |
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