Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil
In Brazil, mineral water is widely consumed and as of yet there have been no studies done in the country that aim to detect enteric viruses in this water source. The aim of this study was to analyze the presence of the human adenovirus (HAdV), the human rotavirus genogroup A (GARV) and the human ent...
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doaj-8f7a8e28d9324cfea53c9d2f2c6195d12020-11-24T23:21:13ZengMDPI AGBeverages2306-57102015-07-011314014810.3390/beverages1030140beverages1030140Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in BrazilVivian Rodrigues dos Santos0Caroline Rigotto1Rodrigo Staggemeier2Andréia Dalla Vecchia3Andréia Henzel4Fernando Rosado Spilki5Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Prédio Vermelho 2° Andar, Sala 205 RS 239, n° 2755, Vila Nova, 93352-000, Novo Hamburgo, RS, BrazilLaboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Prédio Vermelho 2° Andar, Sala 205 RS 239, n° 2755, Vila Nova, 93352-000, Novo Hamburgo, RS, BrazilLaboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Prédio Vermelho 2° Andar, Sala 205 RS 239, n° 2755, Vila Nova, 93352-000, Novo Hamburgo, RS, BrazilLaboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Prédio Vermelho 2° Andar, Sala 205 RS 239, n° 2755, Vila Nova, 93352-000, Novo Hamburgo, RS, BrazilLaboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Prédio Vermelho 2° Andar, Sala 205 RS 239, n° 2755, Vila Nova, 93352-000, Novo Hamburgo, RS, BrazilLaboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Prédio Vermelho 2° Andar, Sala 205 RS 239, n° 2755, Vila Nova, 93352-000, Novo Hamburgo, RS, BrazilIn Brazil, mineral water is widely consumed and as of yet there have been no studies done in the country that aim to detect enteric viruses in this water source. The aim of this study was to analyze the presence of the human adenovirus (HAdV), the human rotavirus genogroup A (GARV) and the human enterovirus (hEV) in mineral water samples from six different brands that are commercialized in southern Brazil, using molecular techniques and comparing the results with bacterial indicators. Samples of 1.5 L and 500 mL were analyzed for viruses through PCR and total and thermotolerant coliforms. Additionally, heterotrophic bacteria were assayed using a commercial kit. The most prevalent virus was adenovirus (32.5%) followed by rotavirus (25%) and enterovirus (17.5%). Total and thermotolerant coliforms were absent in all samples and only three samples out of the 60 analyzed presented heterotrophic bacteria contamination. We conclude that, following the example taken into consideration regarding the public supply of drinking water, stricter measures for microbiological control should also be applied to mineral water so that this actually becomes a safer alternative.http://www.mdpi.com/2306-5710/1/3/140enteric virusesHAdVhEVGARVmineral water |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vivian Rodrigues dos Santos Caroline Rigotto Rodrigo Staggemeier Andréia Dalla Vecchia Andréia Henzel Fernando Rosado Spilki |
spellingShingle |
Vivian Rodrigues dos Santos Caroline Rigotto Rodrigo Staggemeier Andréia Dalla Vecchia Andréia Henzel Fernando Rosado Spilki Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil Beverages enteric viruses HAdV hEV GARV mineral water |
author_facet |
Vivian Rodrigues dos Santos Caroline Rigotto Rodrigo Staggemeier Andréia Dalla Vecchia Andréia Henzel Fernando Rosado Spilki |
author_sort |
Vivian Rodrigues dos Santos |
title |
Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil |
title_short |
Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil |
title_full |
Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil |
title_fullStr |
Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil |
title_full_unstemmed |
Preliminary Evaluation of Enteric Viruses in Bottled Mineral Water Commercialized in Brazil |
title_sort |
preliminary evaluation of enteric viruses in bottled mineral water commercialized in brazil |
publisher |
MDPI AG |
series |
Beverages |
issn |
2306-5710 |
publishDate |
2015-07-01 |
description |
In Brazil, mineral water is widely consumed and as of yet there have been no studies done in the country that aim to detect enteric viruses in this water source. The aim of this study was to analyze the presence of the human adenovirus (HAdV), the human rotavirus genogroup A (GARV) and the human enterovirus (hEV) in mineral water samples from six different brands that are commercialized in southern Brazil, using molecular techniques and comparing the results with bacterial indicators. Samples of 1.5 L and 500 mL were analyzed for viruses through PCR and total and thermotolerant coliforms. Additionally, heterotrophic bacteria were assayed using a commercial kit. The most prevalent virus was adenovirus (32.5%) followed by rotavirus (25%) and enterovirus (17.5%). Total and thermotolerant coliforms were absent in all samples and only three samples out of the 60 analyzed presented heterotrophic bacteria contamination. We conclude that, following the example taken into consideration regarding the public supply of drinking water, stricter measures for microbiological control should also be applied to mineral water so that this actually becomes a safer alternative. |
topic |
enteric viruses HAdV hEV GARV mineral water |
url |
http://www.mdpi.com/2306-5710/1/3/140 |
work_keys_str_mv |
AT vivianrodriguesdossantos preliminaryevaluationofentericvirusesinbottledmineralwatercommercializedinbrazil AT carolinerigotto preliminaryevaluationofentericvirusesinbottledmineralwatercommercializedinbrazil AT rodrigostaggemeier preliminaryevaluationofentericvirusesinbottledmineralwatercommercializedinbrazil AT andreiadallavecchia preliminaryevaluationofentericvirusesinbottledmineralwatercommercializedinbrazil AT andreiahenzel preliminaryevaluationofentericvirusesinbottledmineralwatercommercializedinbrazil AT fernandorosadospilki preliminaryevaluationofentericvirusesinbottledmineralwatercommercializedinbrazil |
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