Bioprospecting of haloalkaliphilic microorganisms isolated from a dried-out maar in the volcano “Hoya Rincón de Parangueo”

• Main Authors: Elizabeth Selene Gómez-Acata, Laura Mariana Ayala-Gámez, Raúl García-Covarrubias, Martha Yvette Torres-Núñez, Liliana Vargas-López, Susana Hernández-Camargo, Itzel Guadalupe Becerril-Rojas, Marcos León-Domínguez, Lizbeth Zurita-García, Yendi E. Navarro-Noya, Eloy Conde-Barajas, Luc Dendooven
• Format: Article
• Language: English
• Published: Universidad De La Salle Bajío 2021-05-01
• Series: Nova Scientia
• Subjects: bioprospection; siderophore; halophile; lipases; Nesterenkonia; hypersaline environments
• Online Access: http://nova_scientia.delasalle.edu.mx/ojs/index.php/Nova/article/view/2553

Halophiles can be found in hypersaline environments and they can synthesize products of industrial interest. Method: In this work, 37 bacterial strains were isolated from the sediment of a dried-out maar of the “Hoya Rincón de Parangueo” (Mexico) on different culture media with 20% NaCl and pH 9, while potential industrial extracellular enzymes were determined. Results: Eight genera Salsuginibacillus, Alkalibacillus, Bacillus, Staphylococcus, Oceanobacillus, Halomonas and Nesterenkonia besides 15 distinct species belonging to Actinobacteria, Firmicutes and Proteobacteria were identified. The L371, LTA7 and SU3 isolates produced proteases, M20, L5, L7, L8, Y1, Y2, M3, M9 and RA lipases, LTA4 and SU3 amylases, while LTA7, Y2, M5, M9 and RA caseinases with enzymatic activity at pH 9. Production of siderophore was found in 17 isolates. Discussion or Conclusion: It is reported for the first experimental evidence of siderophores production by N. alba, Alkalibacillus and Oceanobacillus at 6% NaCl (w/v). Isolates RE, RG1, RG2, RH1, RA and I11 showed 2.8% to 4.1% genetic distance with Nesterenkonia species and might represent new species.