Physicochemical characterization of vibriophage N5

• Main Authors: Sen Anindito, Ghosh Amar N
• Format: Article
• Language: English
• Published: BMC 2005-04-01
• Series: Virology Journal
• Subjects: Vibriophage N5; DNA; <it>Vibrio cholerae</it>; Electron Microscopy; Partial denaturation mapping; Bacteriophage
• Online Access: http://www.virologyj.com/content/2/1/27

<p>Abstract</p> <p>Phage N5 is one of the phages of <it>Vibrio cholerae </it>serovar O1 biotype El Tor (Ghosh, A. N., Ansari, M. Q., and Dutta, G. C. Isolation and morphological characterization of El Tor cholera phages. <it>J. Gen. Virol</it>. 70: 2241–2243, 1989). In the present communication the growth curve, molecular weight and confirmation of the genome, partial denaturation map and restriction endonuclease digestion pattern have been determined. Partial denaturation map indicates that the genome has non-permuted / invariant sequence. Presence of cohesive ends has also been documented.</p>