PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway
Senescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress response...
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doaj-8fa49db169e84f0093e97d031f30f37e2020-11-25T00:54:32ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-09-01199260910.3390/ijms19092609ijms19092609PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related PathwayPing Li0Hong Yang1Gaojing Liu2Wenzhang Ma3Chuanhong Li4Heqiang Huo5Jianfang He6Li Liu7Key Laboratory of Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaKey Laboratory of Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaKey Laboratory of Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaKey Laboratory of Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaNational Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, ChinaMid-Florida Research and Education Center, Department of Environmental Horticulture, University of Florida, Apopka, FL 32703, USAKey Laboratory of Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaKey Laboratory of Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, ChinaSenescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress responses in moss are not well known. Here, we investigated functional roles of SARK isolated from Physcomitrella patens (PpSARK) in salt stress response and senescence. PpSARK transcripts significantly accumulated under NaCl and abscisic acid (ABA) treatments, with higher expression in the moss gametophyte stage. Insertional gain-of-function mutants of PpSARK (PpSARKg) were more tolerant to salt stress and ABA than wild type (WT), whereas senescence of mutants was delayed during the protonema stage. Expression of stress-responsive genes in the ABA related pathway, such as PpABI3, PpABI5, PpPP2C, and PpLEA were significantly higher in PpSARKg and WT under salt stress conditions, suggesting that PpSARK might positively regulate salt tolerance via an ABA-related pathway. Endogenous ABA contents also increased 3-fold under salt stress conditions. These results indicate that PpSARK functions as a positive regulator in salt stress responses, while possibly functioning as a negative regulator in senescence in moss.http://www.mdpi.com/1422-0067/19/9/2609senescencesalt stress responseABASARKmoss (Physcomitrella patens) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ping Li Hong Yang Gaojing Liu Wenzhang Ma Chuanhong Li Heqiang Huo Jianfang He Li Liu |
spellingShingle |
Ping Li Hong Yang Gaojing Liu Wenzhang Ma Chuanhong Li Heqiang Huo Jianfang He Li Liu PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway International Journal of Molecular Sciences senescence salt stress response ABA SARK moss (Physcomitrella patens) |
author_facet |
Ping Li Hong Yang Gaojing Liu Wenzhang Ma Chuanhong Li Heqiang Huo Jianfang He Li Liu |
author_sort |
Ping Li |
title |
PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_short |
PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_full |
PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_fullStr |
PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_full_unstemmed |
PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_sort |
ppsark regulates moss senescence and salt tolerance through aba related pathway |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-09-01 |
description |
Senescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress responses in moss are not well known. Here, we investigated functional roles of SARK isolated from Physcomitrella patens (PpSARK) in salt stress response and senescence. PpSARK transcripts significantly accumulated under NaCl and abscisic acid (ABA) treatments, with higher expression in the moss gametophyte stage. Insertional gain-of-function mutants of PpSARK (PpSARKg) were more tolerant to salt stress and ABA than wild type (WT), whereas senescence of mutants was delayed during the protonema stage. Expression of stress-responsive genes in the ABA related pathway, such as PpABI3, PpABI5, PpPP2C, and PpLEA were significantly higher in PpSARKg and WT under salt stress conditions, suggesting that PpSARK might positively regulate salt tolerance via an ABA-related pathway. Endogenous ABA contents also increased 3-fold under salt stress conditions. These results indicate that PpSARK functions as a positive regulator in salt stress responses, while possibly functioning as a negative regulator in senescence in moss. |
topic |
senescence salt stress response ABA SARK moss (Physcomitrella patens) |
url |
http://www.mdpi.com/1422-0067/19/9/2609 |
work_keys_str_mv |
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