Over-Production, Crystallization, and Preliminary X-ray Crystallographic Analysis of a Coiled-Coil Region in Human Pericentrin
The genes encoding three coiled-coil regions in human pericentrin were gene synthesized with Escherichia coli codon-optimization, and the proteins were successfully over-produced in large quantities using E. coli expression. After verifying that the purified proteins were mostly composed of α-helice...
Main Authors: | Min Ye Kim, Jeong Kuk Park, Yeowon Sim, Doheum Kim, Jeong Yeon Sim, SangYoun Park |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2017-10-01
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Series: | Crystals |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4352/7/10/296 |
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