Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine
Stem cell-based therapies to reconstitute in vivo organ function hold great promise for future clinical applications to a variety of diseases. Hypothyroidism resulting from congenital lack of functional thyrocytes, surgical tissue removal, or gland ablation, represents a particularly attractive endo...
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doaj-8fd7c66b773d4f9e9c9f60c75604b25e2021-04-20T16:07:25ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922021-04-011210.3389/fendo.2021.666565666565Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative MedicineAlberto Posabella0Alberto Posabella1Andrea B. Alber2Hendrik J. Undeutsch3Raoul A. Droeser4Anthony N. Hollenberg5Laertis Ikonomou6Laertis Ikonomou7Laertis Ikonomou8Darrell N. Kotton9Darrell N. Kotton10Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA, United StatesUniversity Center of Gastrointestinal and Liver Diseases—Clarunis, University of Basel, Basel, SwitzerlandCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA, United StatesDivision of Endocrinology, Diabetes and Metabolism, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, New York, NY, United StatesUniversity Center of Gastrointestinal and Liver Diseases—Clarunis, University of Basel, Basel, SwitzerlandDivision of Endocrinology, Diabetes and Metabolism, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, New York, NY, United StatesCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA, United StatesThe Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA, United StatesDepartment of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, NY, United StatesCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA, United StatesThe Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA, United StatesStem cell-based therapies to reconstitute in vivo organ function hold great promise for future clinical applications to a variety of diseases. Hypothyroidism resulting from congenital lack of functional thyrocytes, surgical tissue removal, or gland ablation, represents a particularly attractive endocrine disease target that may be conceivably cured by transplantation of long-lived functional thyroid progenitors or mature follicular epithelial cells, provided a source of autologous cells can be generated and a variety of technical and biological challenges can be surmounted. Here we review the emerging literature indicating that thyroid follicular epithelial cells can now be engineered in vitro from the pluripotent stem cells (PSCs) of mice, normal humans, or patients with congenital hypothyroidism. We review the in vivo embryonic development of the thyroid gland and explain how emerging discoveries in developmental biology have been utilized as a roadmap for driving PSCs, which resemble cells of the early embryo, into mature functional thyroid follicles in vitro. Finally, we discuss the bioengineering, biological, and clinical hurdles that now need to be addressed if the goals of life-long cure of hypothyroidism through cell- and/or gene-based therapies are to be attained.https://www.frontiersin.org/articles/10.3389/fendo.2021.666565/fullthyroid follicular cellscongenital hypothyroidismpluripotent stem cellsregenerative medicinedirected differentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alberto Posabella Alberto Posabella Andrea B. Alber Hendrik J. Undeutsch Raoul A. Droeser Anthony N. Hollenberg Laertis Ikonomou Laertis Ikonomou Laertis Ikonomou Darrell N. Kotton Darrell N. Kotton |
spellingShingle |
Alberto Posabella Alberto Posabella Andrea B. Alber Hendrik J. Undeutsch Raoul A. Droeser Anthony N. Hollenberg Laertis Ikonomou Laertis Ikonomou Laertis Ikonomou Darrell N. Kotton Darrell N. Kotton Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine Frontiers in Endocrinology thyroid follicular cells congenital hypothyroidism pluripotent stem cells regenerative medicine directed differentiation |
author_facet |
Alberto Posabella Alberto Posabella Andrea B. Alber Hendrik J. Undeutsch Raoul A. Droeser Anthony N. Hollenberg Laertis Ikonomou Laertis Ikonomou Laertis Ikonomou Darrell N. Kotton Darrell N. Kotton |
author_sort |
Alberto Posabella |
title |
Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine |
title_short |
Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine |
title_full |
Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine |
title_fullStr |
Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine |
title_full_unstemmed |
Derivation of Thyroid Follicular Cells From Pluripotent Stem Cells: Insights From Development and Implications for Regenerative Medicine |
title_sort |
derivation of thyroid follicular cells from pluripotent stem cells: insights from development and implications for regenerative medicine |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Endocrinology |
issn |
1664-2392 |
publishDate |
2021-04-01 |
description |
Stem cell-based therapies to reconstitute in vivo organ function hold great promise for future clinical applications to a variety of diseases. Hypothyroidism resulting from congenital lack of functional thyrocytes, surgical tissue removal, or gland ablation, represents a particularly attractive endocrine disease target that may be conceivably cured by transplantation of long-lived functional thyroid progenitors or mature follicular epithelial cells, provided a source of autologous cells can be generated and a variety of technical and biological challenges can be surmounted. Here we review the emerging literature indicating that thyroid follicular epithelial cells can now be engineered in vitro from the pluripotent stem cells (PSCs) of mice, normal humans, or patients with congenital hypothyroidism. We review the in vivo embryonic development of the thyroid gland and explain how emerging discoveries in developmental biology have been utilized as a roadmap for driving PSCs, which resemble cells of the early embryo, into mature functional thyroid follicles in vitro. Finally, we discuss the bioengineering, biological, and clinical hurdles that now need to be addressed if the goals of life-long cure of hypothyroidism through cell- and/or gene-based therapies are to be attained. |
topic |
thyroid follicular cells congenital hypothyroidism pluripotent stem cells regenerative medicine directed differentiation |
url |
https://www.frontiersin.org/articles/10.3389/fendo.2021.666565/full |
work_keys_str_mv |
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