Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique
Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, Crimean-Congo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present...
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doaj-8fe01b57490848fba104ace671cda8c22020-11-25T00:35:05ZengTaylor & Francis GroupInfection Ecology & Epidemiology2000-86862018-01-018110.1080/20008686.2018.14785851478585Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from MozambiqueHarindranath Cholleti0Juliette Hayer1Fernando Chanisso Mulandane2Kerstin Falk3Jose Fafetine4Mikael Berg5Anne-Lie Blomström6Swedish University of Agricultural Sciences (SLU)Swedish University of Agricultural SciencesBiotechnology Center, Eduardo Mondlane UniversityTumor and Cell Biology (MTC), Karolinska InstituteBiotechnology Center, Eduardo Mondlane UniversitySwedish University of Agricultural Sciences (SLU)Swedish University of Agricultural Sciences (SLU)Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, Crimean-Congo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present in Rhipicephalus spp. ticks collected in the Zambezi Valley of Mozambique. Methods: The RNA was amplified with sequence-independent single primer amplification (SISPA) and high-throughput sequencing was performed on the Ion Torrent platform. The generated sequences were subjected to quality check and classfied by BLAST. CodonCode aligner and SeqMan were used to assemble the sequences. Results: The majority of viral sequences showed closest sequence identity to the Orthomyxoviridae family, although viruses similar to the Parvoviridae and Coronaviridae were also identified. Nearly complete sequences of five orthomyxoviral segments (HA, NP, PB1, PB2, and PA) were obtained and these showed an amino acid identity of 32–52% to known quaranjaviruses. The sequences were most closely related to the Wellfleet Bay virus, detected and isolated from common eider during a mortality event in the USA. Conclusions: In summary, this study has identified a highly divergent virus with in the Orthomyxoviridae family associated with Rhipicephalus ticks from Mozambique. Further genetic and biological studies are needed in order to investigate potential pathogenesis of the identified orthomyxovirus.http://dx.doi.org/10.1080/20008686.2018.1478585TicksarthropodsRhipicephalusquaranjavirusviral metagenomicsMozambique |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Harindranath Cholleti Juliette Hayer Fernando Chanisso Mulandane Kerstin Falk Jose Fafetine Mikael Berg Anne-Lie Blomström |
spellingShingle |
Harindranath Cholleti Juliette Hayer Fernando Chanisso Mulandane Kerstin Falk Jose Fafetine Mikael Berg Anne-Lie Blomström Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique Infection Ecology & Epidemiology Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique |
author_facet |
Harindranath Cholleti Juliette Hayer Fernando Chanisso Mulandane Kerstin Falk Jose Fafetine Mikael Berg Anne-Lie Blomström |
author_sort |
Harindranath Cholleti |
title |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_short |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_full |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_fullStr |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_full_unstemmed |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_sort |
viral metagenomics reveals the presence of highly divergent quaranjavirus in rhipicephalus ticks from mozambique |
publisher |
Taylor & Francis Group |
series |
Infection Ecology & Epidemiology |
issn |
2000-8686 |
publishDate |
2018-01-01 |
description |
Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, Crimean-Congo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present in Rhipicephalus spp. ticks collected in the Zambezi Valley of Mozambique. Methods: The RNA was amplified with sequence-independent single primer amplification (SISPA) and high-throughput sequencing was performed on the Ion Torrent platform. The generated sequences were subjected to quality check and classfied by BLAST. CodonCode aligner and SeqMan were used to assemble the sequences. Results: The majority of viral sequences showed closest sequence identity to the Orthomyxoviridae family, although viruses similar to the Parvoviridae and Coronaviridae were also identified. Nearly complete sequences of five orthomyxoviral segments (HA, NP, PB1, PB2, and PA) were obtained and these showed an amino acid identity of 32–52% to known quaranjaviruses. The sequences were most closely related to the Wellfleet Bay virus, detected and isolated from common eider during a mortality event in the USA. Conclusions: In summary, this study has identified a highly divergent virus with in the Orthomyxoviridae family associated with Rhipicephalus ticks from Mozambique. Further genetic and biological studies are needed in order to investigate potential pathogenesis of the identified orthomyxovirus. |
topic |
Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique |
url |
http://dx.doi.org/10.1080/20008686.2018.1478585 |
work_keys_str_mv |
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