Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors
Activation of Ca2+ release channels/ryanodine receptors (RyR) by the inward Ca2+ current (I Ca) gives rise to Ca2+-induced Ca2+ release (CICR), the amplifying Ca2+ signaling mechanism that triggers contraction of the heart. CICR, in theory, is a high-gain, self-regenerating process, but an unidentif...
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doaj-8fe745a6af9748f085f395e8731422532020-11-24T21:40:09ZengBMCBiological Research0716-97600717-62872004-01-01374609612Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptorsEMILY F FARRELLANAID ANTARAMIANNANCY BENKUSKYXINSHENG ZHUANGÉLICA RUEDAANA M GÓMEZHÉCTOR H VALDIVIAActivation of Ca2+ release channels/ryanodine receptors (RyR) by the inward Ca2+ current (I Ca) gives rise to Ca2+-induced Ca2+ release (CICR), the amplifying Ca2+ signaling mechanism that triggers contraction of the heart. CICR, in theory, is a high-gain, self-regenerating process, but an unidentified mechanism stabilizes it in vivo. Sorcin, a 21.6 kDa Ca2+-binding protein, binds to cardiac RyRs with high affinity and completely inhibits channel activity. Sorcin significantly inhibits both the spontaneous activity of RyRs in quiescent cells (visualized as Ca2+ sparks) and the I Ca-triggered activity of RyRs that gives rise to [Ca2+]i transients. Since sorcin decreases the amplitude of the [Ca2+]i transient without affecting the amplitude of I Ca, the overall effect of sorcin is to reduce the "gain" of excitation-contraction coupling. Immunocytochemical staining shows that sorcin localizes to the dyadic space of ventricular cardiac myocytes. Ca2+ induces conformational changes and promotes translocation of sorcin between soluble and membranous compartments, but the [Ca2+] required for the latter process (ED50 = ~200 mM) appears to be reached only within the dyadic space. Thus, sorcin is a potent inhibitor of both spontaneous and I Ca-triggered RyR activity and may play a role in helping terminate the positive feedback loop of CICR.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400015Sorcinryanodine receptorsCICRdihydropyridine receptorsarcoplasmic reticulum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
EMILY F FARRELL ANAID ANTARAMIAN NANCY BENKUSKY XINSHENG ZHU ANGÉLICA RUEDA ANA M GÓMEZ HÉCTOR H VALDIVIA |
spellingShingle |
EMILY F FARRELL ANAID ANTARAMIAN NANCY BENKUSKY XINSHENG ZHU ANGÉLICA RUEDA ANA M GÓMEZ HÉCTOR H VALDIVIA Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors Biological Research Sorcin ryanodine receptors CICR dihydropyridine receptor sarcoplasmic reticulum |
author_facet |
EMILY F FARRELL ANAID ANTARAMIAN NANCY BENKUSKY XINSHENG ZHU ANGÉLICA RUEDA ANA M GÓMEZ HÉCTOR H VALDIVIA |
author_sort |
EMILY F FARRELL |
title |
Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors |
title_short |
Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors |
title_full |
Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors |
title_fullStr |
Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors |
title_full_unstemmed |
Regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors |
title_sort |
regulation of cardiac excitation-contraction coupling by sorcin, a novel modulator of ryanodine receptors |
publisher |
BMC |
series |
Biological Research |
issn |
0716-9760 0717-6287 |
publishDate |
2004-01-01 |
description |
Activation of Ca2+ release channels/ryanodine receptors (RyR) by the inward Ca2+ current (I Ca) gives rise to Ca2+-induced Ca2+ release (CICR), the amplifying Ca2+ signaling mechanism that triggers contraction of the heart. CICR, in theory, is a high-gain, self-regenerating process, but an unidentified mechanism stabilizes it in vivo. Sorcin, a 21.6 kDa Ca2+-binding protein, binds to cardiac RyRs with high affinity and completely inhibits channel activity. Sorcin significantly inhibits both the spontaneous activity of RyRs in quiescent cells (visualized as Ca2+ sparks) and the I Ca-triggered activity of RyRs that gives rise to [Ca2+]i transients. Since sorcin decreases the amplitude of the [Ca2+]i transient without affecting the amplitude of I Ca, the overall effect of sorcin is to reduce the "gain" of excitation-contraction coupling. Immunocytochemical staining shows that sorcin localizes to the dyadic space of ventricular cardiac myocytes. Ca2+ induces conformational changes and promotes translocation of sorcin between soluble and membranous compartments, but the [Ca2+] required for the latter process (ED50 = ~200 mM) appears to be reached only within the dyadic space. Thus, sorcin is a potent inhibitor of both spontaneous and I Ca-triggered RyR activity and may play a role in helping terminate the positive feedback loop of CICR. |
topic |
Sorcin ryanodine receptors CICR dihydropyridine receptor sarcoplasmic reticulum |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400015 |
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