Immune microenvironment composition in non‐small cell lung cancer and its association with survival
Abstract Objectives In non‐small cell lung cancer (NSCLC), the immune system and possibly its composition affect survival. In this in silico study, the immune infiltrate composition in NSCLC patients was evaluated. Methods Gene expression data of tumors from early NSCLC patients were obtained from G...
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doaj-900de82e78564084829bf0d7c620ad6a2020-11-25T03:29:05ZengWileyClinical & Translational Immunology2050-00682020-01-0196n/an/a10.1002/cti2.1142Immune microenvironment composition in non‐small cell lung cancer and its association with survivalMenno Tamminga0Thijo Jeroen N Hiltermann1Ed Schuuring2Wim Timens3Rudolf SN Fehrmann4Harry JM Groen5Department of Pulmonary Diseases University Medical Center Groningen University of Groningen Groningen The NetherlandsDepartment of Pulmonary Diseases University Medical Center Groningen University of Groningen Groningen The NetherlandsDepartment of Pathology and Medical Biology University Medical Center Groningen University of Groningen Groningen The NetherlandsDepartment of Pathology and Medical Biology University Medical Center Groningen University of Groningen Groningen The NetherlandsDepartment of Medical Oncology University Medical Center Groningen University of Groningen Groningen The NetherlandsDepartment of Pulmonary Diseases University Medical Center Groningen University of Groningen Groningen The NetherlandsAbstract Objectives In non‐small cell lung cancer (NSCLC), the immune system and possibly its composition affect survival. In this in silico study, the immune infiltrate composition in NSCLC patients was evaluated. Methods Gene expression data of tumors from early NSCLC patients were obtained from Gene Expression Omnibus (GEO). With CIBERSORT, 22 immune cell fractions were estimated. Results The immune infiltrate of 1430 pretreatment NSCLC patients contained mostly plasma cells, macrophages and CD8 T cells. Higher fractions of resting mast and CD4 T‐helper cells were associated with longer overall survival (OS) (HR = 0.95, P < 0.01; HR = 0.98, = 0.04, respectively) and higher fractions of M2 macrophages and active dendritic cells with shorter survival (HR = 1.02, P = 0.03; HR = 1.03, P = 0.05, respectively). Adenocarcinoma patients with survival data (n = 587) showed higher fractions of resting mast and resting CD4 T cells, and lower M0 macrophages than squamous cell carcinoma (n = 254), which were associated with OS (HR = 0.95, P = 0.04; HR = 0.97, P = 0.01; HR = 1.03, P = 0.01, respectively). Fractions of memory B cells, naïve CD4 T cells and neutrophils had different associations with survival depending on the subtype. Smokers had had higher fractions of regulatory T cell, follicular helper T cell, neutrophil and M2 macrophage, which were associated with shorter survival (HR = 1.3, P < 0.01; HR = 1.13, P = 0.02; HR = 1.09, P = 0.03; HR = 1.04, P = 0.02, respectively). Conclusion Pretreatment differences in immune cell composition in NSCLC are associated with survival and depend on smoking status and histological subtype. Smokers' immune composition is associated with lower survival.https://doi.org/10.1002/cti2.1142adenocarcinomabiostatisticsimmune microenvironmentnon‐small cell lung cancersmokingsquamous cell carcinoma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Menno Tamminga Thijo Jeroen N Hiltermann Ed Schuuring Wim Timens Rudolf SN Fehrmann Harry JM Groen |
spellingShingle |
Menno Tamminga Thijo Jeroen N Hiltermann Ed Schuuring Wim Timens Rudolf SN Fehrmann Harry JM Groen Immune microenvironment composition in non‐small cell lung cancer and its association with survival Clinical & Translational Immunology adenocarcinoma biostatistics immune microenvironment non‐small cell lung cancer smoking squamous cell carcinoma |
author_facet |
Menno Tamminga Thijo Jeroen N Hiltermann Ed Schuuring Wim Timens Rudolf SN Fehrmann Harry JM Groen |
author_sort |
Menno Tamminga |
title |
Immune microenvironment composition in non‐small cell lung cancer and its association with survival |
title_short |
Immune microenvironment composition in non‐small cell lung cancer and its association with survival |
title_full |
Immune microenvironment composition in non‐small cell lung cancer and its association with survival |
title_fullStr |
Immune microenvironment composition in non‐small cell lung cancer and its association with survival |
title_full_unstemmed |
Immune microenvironment composition in non‐small cell lung cancer and its association with survival |
title_sort |
immune microenvironment composition in non‐small cell lung cancer and its association with survival |
publisher |
Wiley |
series |
Clinical & Translational Immunology |
issn |
2050-0068 |
publishDate |
2020-01-01 |
description |
Abstract Objectives In non‐small cell lung cancer (NSCLC), the immune system and possibly its composition affect survival. In this in silico study, the immune infiltrate composition in NSCLC patients was evaluated. Methods Gene expression data of tumors from early NSCLC patients were obtained from Gene Expression Omnibus (GEO). With CIBERSORT, 22 immune cell fractions were estimated. Results The immune infiltrate of 1430 pretreatment NSCLC patients contained mostly plasma cells, macrophages and CD8 T cells. Higher fractions of resting mast and CD4 T‐helper cells were associated with longer overall survival (OS) (HR = 0.95, P < 0.01; HR = 0.98, = 0.04, respectively) and higher fractions of M2 macrophages and active dendritic cells with shorter survival (HR = 1.02, P = 0.03; HR = 1.03, P = 0.05, respectively). Adenocarcinoma patients with survival data (n = 587) showed higher fractions of resting mast and resting CD4 T cells, and lower M0 macrophages than squamous cell carcinoma (n = 254), which were associated with OS (HR = 0.95, P = 0.04; HR = 0.97, P = 0.01; HR = 1.03, P = 0.01, respectively). Fractions of memory B cells, naïve CD4 T cells and neutrophils had different associations with survival depending on the subtype. Smokers had had higher fractions of regulatory T cell, follicular helper T cell, neutrophil and M2 macrophage, which were associated with shorter survival (HR = 1.3, P < 0.01; HR = 1.13, P = 0.02; HR = 1.09, P = 0.03; HR = 1.04, P = 0.02, respectively). Conclusion Pretreatment differences in immune cell composition in NSCLC are associated with survival and depend on smoking status and histological subtype. Smokers' immune composition is associated with lower survival. |
topic |
adenocarcinoma biostatistics immune microenvironment non‐small cell lung cancer smoking squamous cell carcinoma |
url |
https://doi.org/10.1002/cti2.1142 |
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