Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture.
The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production...
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doaj-9019142f87164e0c970120c6a04477e32020-11-24T21:50:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11249610.1371/journal.pone.0112496Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture.Florian NoulinJaved Karim ManesiaAnna Rosanas-UrgellAnnette ErhartCéline BorlonJan Van Den AbbeeleUmberto d'AlessandroCatherine M VerfaillieThe predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.http://europepmc.org/articles/PMC4231068?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Florian Noulin Javed Karim Manesia Anna Rosanas-Urgell Annette Erhart Céline Borlon Jan Van Den Abbeele Umberto d'Alessandro Catherine M Verfaillie |
spellingShingle |
Florian Noulin Javed Karim Manesia Anna Rosanas-Urgell Annette Erhart Céline Borlon Jan Van Den Abbeele Umberto d'Alessandro Catherine M Verfaillie Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. PLoS ONE |
author_facet |
Florian Noulin Javed Karim Manesia Anna Rosanas-Urgell Annette Erhart Céline Borlon Jan Van Den Abbeele Umberto d'Alessandro Catherine M Verfaillie |
author_sort |
Florian Noulin |
title |
Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
title_short |
Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
title_full |
Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
title_fullStr |
Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
title_full_unstemmed |
Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture. |
title_sort |
hematopoietic stem/progenitor cell sources to generate reticulocytes for plasmodium vivax culture. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies. |
url |
http://europepmc.org/articles/PMC4231068?pdf=render |
work_keys_str_mv |
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