Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells
Purpose. Type 2 diabetes consists of progressive hyperglycemia and insulin resistance, which could result from glucose toxicity, inflammatory cytokines, and oxidative stress. In the present study we investigated the effect of Gelam honey and quercetin on the oxidative stress-induced inflammatory pat...
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Online Access: | http://dx.doi.org/10.1155/2016/5843615 |
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doaj-903901dfdb8e4a74ab79e63707c0309c2020-11-24T22:06:33ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882016-01-01201610.1155/2016/58436155843615Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster CellsSher Zaman Safi0Kalaivani Batumalaie1Rajes Qvist2Kamaruddin Mohd Yusof3Ikram Shah Ismail4Faculty of Medicine, Department of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaFaculty of Medicine, Department of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaFaculty of Medicine, Department of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Molecular Biology and Genetics, Faculty of Arts and Science, Canik Basari University, Samsun, TurkeyFaculty of Medicine, Department of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaPurpose. Type 2 diabetes consists of progressive hyperglycemia and insulin resistance, which could result from glucose toxicity, inflammatory cytokines, and oxidative stress. In the present study we investigated the effect of Gelam honey and quercetin on the oxidative stress-induced inflammatory pathways and the proinflammatory cytokines. Methods. HIT-T15 cells were cultured and preincubated with the extract of Gelam honey (20, 40, 60, and 80 μg/mL), as well as quercetin (20, 40, 60, and 80 μM), prior to stimulation by 20 and 50 mM glucose. Results. HIT-T15 cells cultured under hyperglycemic condition showed a significant increase in the inflammatory pathways by phosphorylating JNK, IKK-β, and IRS-1 at Ser307 (p<0.05). There was a significant decrease in the phosphorylation of Akt at Ser473 (p<0.05). Pretreatment with Gelam honey and quercetin reduced the expression of phosphorylated JNK, IKK-β, and IRS-1, thereby significantly reducing the expression of proinflammatory cytokines like TNF-α, IL-6, and IL-1β (p<0.05). At the same time there was a significant increase in the phosphorylated Akt showing the protective effects against inflammation and insulin resistance (p<0.05). In conclusion, our data suggest the potential use of the extract from Gelam honey and quercetin in modulating the inflammation induced insulin signaling pathways.http://dx.doi.org/10.1155/2016/5843615 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sher Zaman Safi Kalaivani Batumalaie Rajes Qvist Kamaruddin Mohd Yusof Ikram Shah Ismail |
spellingShingle |
Sher Zaman Safi Kalaivani Batumalaie Rajes Qvist Kamaruddin Mohd Yusof Ikram Shah Ismail Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells Evidence-Based Complementary and Alternative Medicine |
author_facet |
Sher Zaman Safi Kalaivani Batumalaie Rajes Qvist Kamaruddin Mohd Yusof Ikram Shah Ismail |
author_sort |
Sher Zaman Safi |
title |
Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells |
title_short |
Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells |
title_full |
Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells |
title_fullStr |
Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells |
title_full_unstemmed |
Gelam Honey Attenuates the Oxidative Stress-Induced Inflammatory Pathways in Pancreatic Hamster Cells |
title_sort |
gelam honey attenuates the oxidative stress-induced inflammatory pathways in pancreatic hamster cells |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2016-01-01 |
description |
Purpose. Type 2 diabetes consists of progressive hyperglycemia and insulin resistance, which could result from glucose toxicity, inflammatory cytokines, and oxidative stress. In the present study we investigated the effect of Gelam honey and quercetin on the oxidative stress-induced inflammatory pathways and the proinflammatory cytokines. Methods. HIT-T15 cells were cultured and preincubated with the extract of Gelam honey (20, 40, 60, and 80 μg/mL), as well as quercetin (20, 40, 60, and 80 μM), prior to stimulation by 20 and 50 mM glucose. Results. HIT-T15 cells cultured under hyperglycemic condition showed a significant increase in the inflammatory pathways by phosphorylating JNK, IKK-β, and IRS-1 at Ser307 (p<0.05). There was a significant decrease in the phosphorylation of Akt at Ser473 (p<0.05). Pretreatment with Gelam honey and quercetin reduced the expression of phosphorylated JNK, IKK-β, and IRS-1, thereby significantly reducing the expression of proinflammatory cytokines like TNF-α, IL-6, and IL-1β (p<0.05). At the same time there was a significant increase in the phosphorylated Akt showing the protective effects against inflammation and insulin resistance (p<0.05). In conclusion, our data suggest the potential use of the extract from Gelam honey and quercetin in modulating the inflammation induced insulin signaling pathways. |
url |
http://dx.doi.org/10.1155/2016/5843615 |
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