Effects of Astragalus injection on cervical immortalized epithelial cell growth and its cell cycle regulation mechanism

• Main Authors: Ling Lyu, Chen-Guang Xiao, Li Zhang, Li-Ya Jin, Qing Liu, Zhi-Rong Dai
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-02-01
• Series: Journal of Hainan Medical University
• Subjects: Cervical cancer; Cervical intraepithelial neoplasia; Astragalus injection; Cell cycle.
• Online Access: http://www.jhmuweb.net/PDF/201604/5.pdf

Objective: To study the regulating effect of Astragalus injection on cervical immortalized epithelial cell growth and cell cycle regulation mechanism. Methods: Cervical immortalized epithelial cells (H8 cells) were selected for experiment and processed with different concentrations of Astragalus injection, MTT method was used to detect cell viability, flow cytometry was used to detect the percentages of different cell cycles and the contents of different immune cell subsets, and PCR method was used to detect mRNA contents of cell cycle-related proteins. Results: Astragalus injection could reduce cell viability in a dosedependent manner, and the inhibiting effect of 200 μg/mL concentration was the most significant; NK cell and CD3+CD4+CD8-T cell contents as well as CD3+CD4+CD8-/CD3+CD4- CD8+T cell percentage of 200 μg/mL Astragalus–processing group was higher than those of 0 μg/mL Astragalus–processing group, and CD3+CD4-CD8+T cell content was lower than that of 0 μg/mL Astragalus–processing group; percentages of G0/G1 phase and S phase cells as well as mRNA contents of cyclinA, cyclinB and cyclinD1 of 200 μg/mL Astragalus–processing group were lower than those of 0 μg/mL Astragalus–processing group, and percentage of G2/M phase cell was higher than that of 0 μg/mL Astragalus–processing group. Conclusion: Astragalus injection has inhibiting effect on the growth of cervical immortalized epithelial cells, and its mechanisms include regulating immune cell contents, arresting cell cycle and down-regulating cell cycle-related protein expression.