Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis

Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis

Four different normalisation techniques were applied for the corrections of fluorescence data generated by a cDNA microarray experiment. Correction for inaccurate signals and possible bias induced by fluorescence intensity, background intensity and dye effect were used in different combinations. Res...

Full description

Bibliographic Details
Main Authors: N. P. P. Macciotta, N. Bacciu, C. Dimauro
Format: Article
Language:English
Published: Taylor & Francis Group 2010-04-01
Series:Italian Journal of Animal Science
Online Access:http://www.aspajournal.it/index.php/ijas/article/view/1397
id doaj-905b96b1ddd44947bba9e7cd19ba52d9
record_format Article
spelling doaj-905b96b1ddd44947bba9e7cd19ba52d92020-11-25T01:12:13ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2010-04-0161s12212410.4081/ijas.2007.1s.122Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysisN. P. P. MacciottaN. BacciuC. DimauroFour different normalisation techniques were applied for the corrections of fluorescence data generated by a cDNA microarray experiment. Correction for inaccurate signals and possible bias induced by fluorescence intensity, background intensity and dye effect were used in different combinations. Results of the present study highlight a pronounced role for the normalisation techniques in the absolute number of genes different expressed and a low concordance between different methods. Moreover, a significant effect of the dependent variable used, mean or median fluorescence intensity, was observed.http://www.aspajournal.it/index.php/ijas/article/view/1397
collection DOAJ
language English
format Article
sources DOAJ
author N. P. P. Macciotta
N. Bacciu
C. Dimauro
spellingShingle N. P. P. Macciotta
N. Bacciu
C. Dimauro
Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis
Italian Journal of Animal Science
author_facet N. P. P. Macciotta
N. Bacciu
C. Dimauro
author_sort N. P. P. Macciotta
title Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis
title_short Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis
title_full Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis
title_fullStr Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis
title_full_unstemmed Effect of normalisation on detection of differentially expressed genes in cDNA microarray data analysis
title_sort effect of normalisation on detection of differentially expressed genes in cdna microarray data analysis
publisher Taylor & Francis Group
series Italian Journal of Animal Science
issn 1594-4077
1828-051X
publishDate 2010-04-01
description Four different normalisation techniques were applied for the corrections of fluorescence data generated by a cDNA microarray experiment. Correction for inaccurate signals and possible bias induced by fluorescence intensity, background intensity and dye effect were used in different combinations. Results of the present study highlight a pronounced role for the normalisation techniques in the absolute number of genes different expressed and a low concordance between different methods. Moreover, a significant effect of the dependent variable used, mean or median fluorescence intensity, was observed.
url http://www.aspajournal.it/index.php/ijas/article/view/1397
work_keys_str_mv AT nppmacciotta effectofnormalisationondetectionofdifferentiallyexpressedgenesincdnamicroarraydataanalysis
AT nbacciu effectofnormalisationondetectionofdifferentiallyexpressedgenesincdnamicroarraydataanalysis
AT cdimauro effectofnormalisationondetectionofdifferentiallyexpressedgenesincdnamicroarraydataanalysis
_version_ 1725167777756479488

Similar Items