Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries)
The FecB gene has been discovered as an important gene in sheep for its high relationship with the ovulation rate, but its regulatory mechanism remains unknown. In the present study, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) techniques were adop...
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doaj-9070aa0f5a9943509430f3c7067345b42020-11-24T21:49:58ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-02-0119253910.3390/ijms19020539ijms19020539Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries)Xiaofei Guo0Xiangyu Wang1Ran Di2Qiuyue Liu3Wenping Hu4Xiaoyun He5Jiarui Yu6Xiaosheng Zhang7Jinlong Zhang8Katarzyna Broniowska9Wei Chen10Changxin Wu11Mingxing Chu12Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaTianjin Institute of Animal Sciences, Tianjin 300381, ChinaTianjin Institute of Animal Sciences, Tianjin 300381, ChinaMetabolon, Inc., Morrisville, NC 27560, USAShanghai Applied Protein Technology Co., Ltd., Shanghai 200233, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaThe FecB gene has been discovered as an important gene in sheep for its high relationship with the ovulation rate, but its regulatory mechanism remains unknown. In the present study, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) techniques were adopted to detect the metabolic effects of FecB gene in follicular fluid (FF) and ovarian vein serum (OVS) in Small Tail Han (STH) sheep. ANOVA and random forest statistical methods were employed for the identification of important metabolic pathways and biomarkers. Changes in amino acid metabolism, redox environment, and energy metabolism were observed in FF from the three FecB genotype STH ewes. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) showed that metabolic effects of FecB gene are more pronounced in FF than in OVS. Therefore, the difference of the metabolic profile in FF is also affected by the FecB genotypes. In Spearman correlation analysis, key metabolites (e.g., glucose 6-phosphate, glucose 1-phosphate, aspartate, asparagine, glutathione oxidized (GSSG), cysteine-glutathione disulfide, γ-glutamylglutamine, and 2-hydrosybutyrate) in ovine FF samples showed a significant correlation with the ovulation rate. Our findings will help to explain the metabolic mechanism of high prolificacy ewes and benefit fertility identification.http://www.mdpi.com/1422-0067/19/2/539FecBfollicular fluidovarian vein serummetabolic profileovulation ratesheep |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaofei Guo Xiangyu Wang Ran Di Qiuyue Liu Wenping Hu Xiaoyun He Jiarui Yu Xiaosheng Zhang Jinlong Zhang Katarzyna Broniowska Wei Chen Changxin Wu Mingxing Chu |
spellingShingle |
Xiaofei Guo Xiangyu Wang Ran Di Qiuyue Liu Wenping Hu Xiaoyun He Jiarui Yu Xiaosheng Zhang Jinlong Zhang Katarzyna Broniowska Wei Chen Changxin Wu Mingxing Chu Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries) International Journal of Molecular Sciences FecB follicular fluid ovarian vein serum metabolic profile ovulation rate sheep |
author_facet |
Xiaofei Guo Xiangyu Wang Ran Di Qiuyue Liu Wenping Hu Xiaoyun He Jiarui Yu Xiaosheng Zhang Jinlong Zhang Katarzyna Broniowska Wei Chen Changxin Wu Mingxing Chu |
author_sort |
Xiaofei Guo |
title |
Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries) |
title_short |
Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries) |
title_full |
Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries) |
title_fullStr |
Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries) |
title_full_unstemmed |
Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries) |
title_sort |
metabolic effects of fecb gene on follicular fluid and ovarian vein serum in sheep (ovis aries) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-02-01 |
description |
The FecB gene has been discovered as an important gene in sheep for its high relationship with the ovulation rate, but its regulatory mechanism remains unknown. In the present study, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) techniques were adopted to detect the metabolic effects of FecB gene in follicular fluid (FF) and ovarian vein serum (OVS) in Small Tail Han (STH) sheep. ANOVA and random forest statistical methods were employed for the identification of important metabolic pathways and biomarkers. Changes in amino acid metabolism, redox environment, and energy metabolism were observed in FF from the three FecB genotype STH ewes. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) showed that metabolic effects of FecB gene are more pronounced in FF than in OVS. Therefore, the difference of the metabolic profile in FF is also affected by the FecB genotypes. In Spearman correlation analysis, key metabolites (e.g., glucose 6-phosphate, glucose 1-phosphate, aspartate, asparagine, glutathione oxidized (GSSG), cysteine-glutathione disulfide, γ-glutamylglutamine, and 2-hydrosybutyrate) in ovine FF samples showed a significant correlation with the ovulation rate. Our findings will help to explain the metabolic mechanism of high prolificacy ewes and benefit fertility identification. |
topic |
FecB follicular fluid ovarian vein serum metabolic profile ovulation rate sheep |
url |
http://www.mdpi.com/1422-0067/19/2/539 |
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