Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease
Over the last decades, cerium oxide nanoparticles (CeO<sub>2</sub> NPs) have gained great interest due to their potential applications, mainly in the fields of agriculture and biomedicine. Promising effects of CeO<sub>2</sub> NPs are recently shown in some neurodegenerative d...
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doaj-9088e00056224009b4a1574a03ad7a1a2020-11-25T01:45:17ZengMDPI AGNanomaterials2079-49912020-01-0110223510.3390/nano10020235nano10020235Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s DiseaseRoberta Ruotolo0Giuseppe De Giorgio1Ilaria Minato2Massimiliano G. Bianchi3Ovidio Bussolati4Nelson Marmiroli5Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, ItalyDepartment of Medicine and Surgery, University of Parma, 43125 Parma, ItalyDepartment of Medicine and Surgery, University of Parma, 43125 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, ItalyOver the last decades, cerium oxide nanoparticles (CeO<sub>2</sub> NPs) have gained great interest due to their potential applications, mainly in the fields of agriculture and biomedicine. Promising effects of CeO<sub>2</sub> NPs are recently shown in some neurodegenerative diseases, but the mechanism of action of these NPs in Parkinson’s disease (PD) remains to be investigated. This issue is addressed in the present study by using a yeast model based on the heterologous expression of the human α-synuclein (α-syn), the major component of Lewy bodies, which represent a neuropathological hallmark of PD. We observed that CeO<sub>2</sub> NPs strongly reduce α-syn-induced toxicity in a dose-dependent manner. This effect is associated with the inhibition of cytoplasmic α-syn foci accumulation, resulting in plasma membrane localization of α-syn after NP treatment. Moreover, CeO<sub>2</sub> NPs counteract the α-syn-induced mitochondrial dysfunction and decrease reactive oxygen species (ROS) production in yeast cells. In vitro binding assay using cell lysates showed that α-syn is adsorbed on the surface of CeO<sub>2</sub> NPs, suggesting that these NPs may act as a strong inhibitor of α-syn toxicity not only acting as a radical scavenger, but through a direct interaction with α-syn in vivo.https://www.mdpi.com/2079-4991/10/2/235cerium oxide nanoparticlesnanoceriaparkinson’s diseaseα-synucleinyeast modelamyloid aggregatesoligomer detoxificationcluster of lipid vesiclesneurodegenerative disease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roberta Ruotolo Giuseppe De Giorgio Ilaria Minato Massimiliano G. Bianchi Ovidio Bussolati Nelson Marmiroli |
spellingShingle |
Roberta Ruotolo Giuseppe De Giorgio Ilaria Minato Massimiliano G. Bianchi Ovidio Bussolati Nelson Marmiroli Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease Nanomaterials cerium oxide nanoparticles nanoceria parkinson’s disease α-synuclein yeast model amyloid aggregates oligomer detoxification cluster of lipid vesicles neurodegenerative disease |
author_facet |
Roberta Ruotolo Giuseppe De Giorgio Ilaria Minato Massimiliano G. Bianchi Ovidio Bussolati Nelson Marmiroli |
author_sort |
Roberta Ruotolo |
title |
Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease |
title_short |
Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease |
title_full |
Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease |
title_fullStr |
Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease |
title_full_unstemmed |
Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease |
title_sort |
cerium oxide nanoparticles rescue α-synuclein-induced toxicity in a yeast model of parkinson’s disease |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2020-01-01 |
description |
Over the last decades, cerium oxide nanoparticles (CeO<sub>2</sub> NPs) have gained great interest due to their potential applications, mainly in the fields of agriculture and biomedicine. Promising effects of CeO<sub>2</sub> NPs are recently shown in some neurodegenerative diseases, but the mechanism of action of these NPs in Parkinson’s disease (PD) remains to be investigated. This issue is addressed in the present study by using a yeast model based on the heterologous expression of the human α-synuclein (α-syn), the major component of Lewy bodies, which represent a neuropathological hallmark of PD. We observed that CeO<sub>2</sub> NPs strongly reduce α-syn-induced toxicity in a dose-dependent manner. This effect is associated with the inhibition of cytoplasmic α-syn foci accumulation, resulting in plasma membrane localization of α-syn after NP treatment. Moreover, CeO<sub>2</sub> NPs counteract the α-syn-induced mitochondrial dysfunction and decrease reactive oxygen species (ROS) production in yeast cells. In vitro binding assay using cell lysates showed that α-syn is adsorbed on the surface of CeO<sub>2</sub> NPs, suggesting that these NPs may act as a strong inhibitor of α-syn toxicity not only acting as a radical scavenger, but through a direct interaction with α-syn in vivo. |
topic |
cerium oxide nanoparticles nanoceria parkinson’s disease α-synuclein yeast model amyloid aggregates oligomer detoxification cluster of lipid vesicles neurodegenerative disease |
url |
https://www.mdpi.com/2079-4991/10/2/235 |
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