Inhibitory effects of Iranian Cuminum Cyminum Against Amyloid Fibril Formation and Cytotoxicity of Hen Egg white Lysozyme (HEWL) on SK-N-MC Cells
Background and Objective: Protein fibrils are deposited in different tissues as amyloid plaques, and it is strongly believed that they contribute to the pathogenesis of disorders, called amyloidosis. Cuminum cyminum has been used traditionally for dementia, which is a related disease to protein fibr...
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Alborz University of Medical Sciencs
2014-08-01
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doaj-9095fd66860041fbb2fd0232a3a898e02020-11-24T21:02:22ZfasAlborz University of Medical SciencsBihdād1735-66792014-08-0133141152Inhibitory effects of Iranian Cuminum Cyminum Against Amyloid Fibril Formation and Cytotoxicity of Hen Egg white Lysozyme (HEWL) on SK-N-MC CellsT. Salmani0F. Aliakbari1D. Morshedi2 MA in Cellular Biology, Department of Industrial and Environmental Biotechnology, National Institute of Genetic, Engineering and Biotechnology Shahrak-e Pajoohesh, km 15, Tehran-Karaj Highway, Tehran, Iran MA in Cellular Biology, Department of Industrial and Environmental Biotechnology, National Institute of Genetic, Engineering and Biotechnology Shahrak-e Pajoohesh, km 15, Tehran-Karaj Highway, Tehran, Iran PhD in Biochemistry, National Institute of Genetic, Engineering and Biotechnology Shahrak-e Pajoohesh, km 15, Tehran-Karaj Highway, Tehran, Iran Background and Objective: Protein fibrils are deposited in different tissues as amyloid plaques, and it is strongly believed that they contribute to the pathogenesis of disorders, called amyloidosis. Cuminum cyminum has been used traditionally for dementia, which is a related disease to protein fibrillation. The purpose of this study was to investigate the inhibitory effects of Iranian herbal medicinal essential oils, as sources of small molecules, on the protein fibrillation process and their cytotoxicity effects. Materials and Methods: Fibrillation was characterized using circular dichroism spectroscopy, fluorescence assay with thioflavin T, and atomic force microscopy. Cytotoxicity studies were examined on SK-N-MC cell lines by MTT, lactate dehydrogenase and glucose consumption assays. Results: The study showed that the C. cyminum essential oil inhibited fibrillation of the hen egg white lysozyme (HEWL), representing the protein model for fibrillation. This effect was specific, although, other herbal medicinal oils did not demonstrate similar inhibitory effects on protein fibrillation, in fact, sometimes they induced fibrillation. Cytotoxicity assays indicated that the oil alone destroyed the cultivated cells. Conclusion: It is concluded that although C. cyminum have a significant effect on the inhibition of protein fibrillation but causes cell death.http://aums.abzums.ac.ir/browse.php?a_code=A-10-44-2&slc_lang=en&sid=1Anti-fibrillation Essential oil HEWL fibrillation Iranian Cuminum cyminum |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
T. Salmani F. Aliakbari D. Morshedi |
spellingShingle |
T. Salmani F. Aliakbari D. Morshedi Inhibitory effects of Iranian Cuminum Cyminum Against Amyloid Fibril Formation and Cytotoxicity of Hen Egg white Lysozyme (HEWL) on SK-N-MC Cells Bihdād Anti-fibrillation Essential oil HEWL fibrillation Iranian Cuminum cyminum |
author_facet |
T. Salmani F. Aliakbari D. Morshedi |
author_sort |
T. Salmani |
title |
Inhibitory effects of Iranian Cuminum Cyminum Against Amyloid Fibril Formation and Cytotoxicity of Hen Egg white Lysozyme (HEWL) on SK-N-MC Cells |
title_short |
Inhibitory effects of Iranian Cuminum Cyminum Against Amyloid Fibril Formation and Cytotoxicity of Hen Egg white Lysozyme (HEWL) on SK-N-MC Cells |
title_full |
Inhibitory effects of Iranian Cuminum Cyminum Against Amyloid Fibril Formation and Cytotoxicity of Hen Egg white Lysozyme (HEWL) on SK-N-MC Cells |
title_fullStr |
Inhibitory effects of Iranian Cuminum Cyminum Against Amyloid Fibril Formation and Cytotoxicity of Hen Egg white Lysozyme (HEWL) on SK-N-MC Cells |
title_full_unstemmed |
Inhibitory effects of Iranian Cuminum Cyminum Against Amyloid Fibril Formation and Cytotoxicity of Hen Egg white Lysozyme (HEWL) on SK-N-MC Cells |
title_sort |
inhibitory effects of iranian cuminum cyminum against amyloid fibril formation and cytotoxicity of hen egg white lysozyme (hewl) on sk-n-mc cells |
publisher |
Alborz University of Medical Sciencs |
series |
Bihdād |
issn |
1735-6679 |
publishDate |
2014-08-01 |
description |
Background and Objective: Protein fibrils are deposited in different tissues as amyloid plaques, and it is strongly believed that they contribute to the pathogenesis of disorders, called amyloidosis. Cuminum cyminum has been used traditionally for dementia, which is a related disease to protein fibrillation. The purpose of this study was to investigate the inhibitory effects of Iranian herbal medicinal essential oils, as sources of small molecules, on the protein fibrillation process and their cytotoxicity effects. Materials and Methods: Fibrillation was characterized using circular dichroism spectroscopy, fluorescence assay with thioflavin T, and atomic force microscopy. Cytotoxicity studies were examined on SK-N-MC cell lines by MTT, lactate dehydrogenase and glucose consumption assays. Results: The study showed that the C. cyminum essential oil inhibited fibrillation of the hen egg white lysozyme (HEWL), representing the protein model for fibrillation. This effect was specific, although, other herbal medicinal oils did not demonstrate similar inhibitory effects on protein fibrillation, in fact, sometimes they induced fibrillation. Cytotoxicity assays indicated that the oil alone destroyed the cultivated cells. Conclusion: It is concluded that although C. cyminum have a significant effect on the inhibition of protein fibrillation but causes cell death. |
topic |
Anti-fibrillation Essential oil HEWL fibrillation Iranian Cuminum cyminum |
url |
http://aums.abzums.ac.ir/browse.php?a_code=A-10-44-2&slc_lang=en&sid=1 |
work_keys_str_mv |
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