First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America
Objective. To compare the performance of polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests for diagnosing Echinococcus granulosus in dog feces among national reference laboratories in Argentina, Chile, Peru, and Uruguay. Methods. National laboratories affiliated wit...
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Pan American Health Organization
2019-12-01
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doaj-90a3e80e5d394ea7ba8028e0a59e30a22020-11-24T21:56:34ZengPan American Health OrganizationRevista Panamericana de Salud Pública1020-49891680-53482019-12-014311710.26633/RPSP.2019.89rpspFirst inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin AmericaMaría Isabel Jercic0Graciela Santillan1Susana Elola2William Quispe Paredes3Lidia B. Conza Blanco4Noelia Morel5Rodrigo Villegas6Baldomero Molina Flores7Cesar M. Gavidia8Marta Cabrera9Alexandre Guerra dos Santos10Manuel J. Sanchez-Vazquez11Melody J. Maxwell12Marco A. Vigilato13Edmundo Larrieu14Víctor J. Del Rio Vilas15Instituto de Salud Pública de Chile, Santiago de Chile, ChileInstituto Nacional de Enfermedades Infecciosas “Carlos G. Malbran,” Buenos Aires, ArgentinaComisión Nacional de Zoonosis, Montevideo, UruguayInstituto Nacional de Salud, Lima, PeruServicio Nacional de Sanidad Agraria, Lima, PeruComisión Nacional de Zoonosis, Montevideo, UruguayInstituto de Salud Pública de Chile, Santiago de Chile, ChileCentro Panamericano de Fiebre Aftosa, Pan American Health Organization (PAHO) / World Health Organization (WHO), Rio de Janeiro, BrazilFacultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, PeruInstituto Nacional de Enfermedades Infecciosas “Carlos G. Malbran,” Buenos Aires, ArgentinaCentro Panamericano de Fiebre Aftosa, Pan American Health Organization (PAHO) / World Health Organization (WHO), Rio de Janeiro, BrazilCentro Panamericano de Fiebre Aftosa, Pan American Health Organization (PAHO) / World Health Organization (WHO), Rio de Janeiro, BrazilThe Ohio State University, Columbus, Ohio, United States of AmericaPAHO/WHO Country Office, San Salvador, El SalvadorEscuela de Veterinaria, Universidad Nacional de Rio Negro, Choele Choel, ArgentinaSchool of Veterinary Medicine, University of Surrey, Surrey, United KingdomObjective. To compare the performance of polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests for diagnosing Echinococcus granulosus in dog feces among national reference laboratories in Argentina, Chile, Peru, and Uruguay. Methods. National laboratories affiliated with the Ministry of Health/Agriculture of each country exchanged panels of 10 positive/negative samples obtained from their regular national surveillance programs in November 2015 – November 2016. All laboratories applied PCR; two also applied ELISA techniques. Sensitivity and specificity were determined for each laboratory and concordance of results among the laboratories was evaluated by Cohen Kappa coefficient. Results. Poor concordance (3 of 10 paired comparisons had values of Kappa > 0.4), low sensitivity and specificity across all laboratories, and poor performance of both techniques in detecting E. granulosus in canine feces was demonstrated in this study. An ex-post comparison of the laboratories’ test protocols showed substantial heterogeneity that could partially explain poor concordance of results. Conclusion. The results underscore the heterogeneity of canine echinococcosis diagnosis across the region and indicate possible sources of variability. Efforts to standardize canine echinococcosis testing must be included in the plan of action for the Regional Initiative for the Control of Cystic Echinococcosis. Future comparisons with fecal samples of known parasite load are needed.http://iris.paho.org/xmlui/handle/123456789/51732echinococcosisdog diseaseslaboratory proficiency testingsouth america |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María Isabel Jercic Graciela Santillan Susana Elola William Quispe Paredes Lidia B. Conza Blanco Noelia Morel Rodrigo Villegas Baldomero Molina Flores Cesar M. Gavidia Marta Cabrera Alexandre Guerra dos Santos Manuel J. Sanchez-Vazquez Melody J. Maxwell Marco A. Vigilato Edmundo Larrieu Víctor J. Del Rio Vilas |
spellingShingle |
María Isabel Jercic Graciela Santillan Susana Elola William Quispe Paredes Lidia B. Conza Blanco Noelia Morel Rodrigo Villegas Baldomero Molina Flores Cesar M. Gavidia Marta Cabrera Alexandre Guerra dos Santos Manuel J. Sanchez-Vazquez Melody J. Maxwell Marco A. Vigilato Edmundo Larrieu Víctor J. Del Rio Vilas First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America Revista Panamericana de Salud Pública echinococcosis dog diseases laboratory proficiency testing south america |
author_facet |
María Isabel Jercic Graciela Santillan Susana Elola William Quispe Paredes Lidia B. Conza Blanco Noelia Morel Rodrigo Villegas Baldomero Molina Flores Cesar M. Gavidia Marta Cabrera Alexandre Guerra dos Santos Manuel J. Sanchez-Vazquez Melody J. Maxwell Marco A. Vigilato Edmundo Larrieu Víctor J. Del Rio Vilas |
author_sort |
María Isabel Jercic |
title |
First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America |
title_short |
First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America |
title_full |
First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America |
title_fullStr |
First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America |
title_full_unstemmed |
First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America |
title_sort |
first inter-laboratory comparison of echinococcus granulosus sensu lato diagnosis in latin america |
publisher |
Pan American Health Organization |
series |
Revista Panamericana de Salud Pública |
issn |
1020-4989 1680-5348 |
publishDate |
2019-12-01 |
description |
Objective. To compare the performance of polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests for diagnosing Echinococcus granulosus in dog feces among national reference laboratories in Argentina, Chile, Peru, and Uruguay.
Methods. National laboratories affiliated with the Ministry of Health/Agriculture of each country exchanged panels of 10 positive/negative samples obtained from their regular national surveillance programs in November 2015 – November 2016. All laboratories applied PCR; two also applied ELISA techniques. Sensitivity and specificity were determined for each laboratory and concordance of results among the laboratories was evaluated by Cohen Kappa coefficient.
Results. Poor concordance (3 of 10 paired comparisons had values of Kappa > 0.4), low sensitivity and specificity across all laboratories, and poor performance of both techniques in detecting E. granulosus in canine feces was demonstrated in this study. An ex-post comparison of the laboratories’ test protocols showed substantial heterogeneity that could partially explain poor concordance of results.
Conclusion. The results underscore the heterogeneity of canine echinococcosis diagnosis across the region and indicate possible sources of variability. Efforts to standardize canine echinococcosis testing must be included in the plan of action for the Regional Initiative for the Control of Cystic Echinococcosis. Future comparisons with fecal samples of known parasite load are needed. |
topic |
echinococcosis dog diseases laboratory proficiency testing south america |
url |
http://iris.paho.org/xmlui/handle/123456789/51732 |
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