Deciphering the particle specific effects on metabolism in rat liver and plasma from ZnO nanoparticles versus ionic Zn exposure
Toxicity of ZnO nanoparticles (NPs) are often related to the release of Zn2+ ions due to their dissolution. Studies also suggest that the toxicity of ZnO NPs cannot be solely explained by the release of Zn2+ ions; however, there is a lack of direct evidence of ZnO particulate effects. This study com...
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Format: | Article |
Language: | English |
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Elsevier
2020-03-01
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Series: | Environment International |
Online Access: | http://www.sciencedirect.com/science/article/pii/S016041201934142X |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhiling Guo Yali Luo Peng Zhang Andrew J. Chetwynd Heidi Qunhui Xie Fazel Abdolahpur Monikh Wunqun Tao Changjian Xie Yiyun Liu Li Xu Zhiyong Zhang Eugenia Valsami-Jones Iseult Lynch Bin Zhao |
spellingShingle |
Zhiling Guo Yali Luo Peng Zhang Andrew J. Chetwynd Heidi Qunhui Xie Fazel Abdolahpur Monikh Wunqun Tao Changjian Xie Yiyun Liu Li Xu Zhiyong Zhang Eugenia Valsami-Jones Iseult Lynch Bin Zhao Deciphering the particle specific effects on metabolism in rat liver and plasma from ZnO nanoparticles versus ionic Zn exposure Environment International |
author_facet |
Zhiling Guo Yali Luo Peng Zhang Andrew J. Chetwynd Heidi Qunhui Xie Fazel Abdolahpur Monikh Wunqun Tao Changjian Xie Yiyun Liu Li Xu Zhiyong Zhang Eugenia Valsami-Jones Iseult Lynch Bin Zhao |
author_sort |
Zhiling Guo |
title |
Deciphering the particle specific effects on metabolism in rat liver and plasma from ZnO nanoparticles versus ionic Zn exposure |
title_short |
Deciphering the particle specific effects on metabolism in rat liver and plasma from ZnO nanoparticles versus ionic Zn exposure |
title_full |
Deciphering the particle specific effects on metabolism in rat liver and plasma from ZnO nanoparticles versus ionic Zn exposure |
title_fullStr |
Deciphering the particle specific effects on metabolism in rat liver and plasma from ZnO nanoparticles versus ionic Zn exposure |
title_full_unstemmed |
Deciphering the particle specific effects on metabolism in rat liver and plasma from ZnO nanoparticles versus ionic Zn exposure |
title_sort |
deciphering the particle specific effects on metabolism in rat liver and plasma from zno nanoparticles versus ionic zn exposure |
publisher |
Elsevier |
series |
Environment International |
issn |
0160-4120 |
publishDate |
2020-03-01 |
description |
Toxicity of ZnO nanoparticles (NPs) are often related to the release of Zn2+ ions due to their dissolution. Studies also suggest that the toxicity of ZnO NPs cannot be solely explained by the release of Zn2+ ions; however, there is a lack of direct evidence of ZnO particulate effects. This study compared the acute toxicity of ZnO NPs and ZnSO4 following intranasal exposure using a combination of metallomics and metabolomics approaches. Significant accumulation of Zn in the liver was only found in the ZnO NP treatment, with 29% of the newly accumulated Zn in the form of ZnO as revealed by X-ray fine structure spectroscopy (XAFS). This is the first direct evidence suggesting the persistence of ZnO NPs in liver upon intranasal exposure. Although both ZnO NPs and ZnSO4 altered the metabolite profiles, with some overlaps and considerable specificity, of both liver and plasma samples, more and distinct metabolites in the liver and opposite effects in the plasma were altered by ZnO NPs compared with ZnSO4, consistent with no accumulation of Zn detected in liver from ZnSO4. Specifically, a large number of antioxidant-related compounds and energetic substrates were exclusively elevated in the liver of ZnO NP-treated animals. These findings provided direct evidence that persistence of ZnO NPs induced particle-specific effects on the antioxidant systems and energy metabolism pathways. Keywords: Zinc oxide nanoparticles, Zinc ions, Metabolite profile, Energy metabolism, oxidative stress |
url |
http://www.sciencedirect.com/science/article/pii/S016041201934142X |
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doaj-90a5203781284a779b102424e6f74c102020-11-25T02:11:45ZengElsevierEnvironment International0160-41202020-03-01136Deciphering the particle specific effects on metabolism in rat liver and plasma from ZnO nanoparticles versus ionic Zn exposureZhiling Guo0Yali Luo1Peng Zhang2Andrew J. Chetwynd3Heidi Qunhui Xie4Fazel Abdolahpur Monikh5Wunqun Tao6Changjian Xie7Yiyun Liu8Li Xu9Zhiyong Zhang10Eugenia Valsami-Jones11Iseult Lynch12Bin Zhao13State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authors at: 18 Shuangqing Rd, Beijing 100085, China (Bin Zhao). University of Birmingham, Birmingham B15 2TT, UK (Peng Zhang, Zhiling Guo).State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UK; Corresponding authors at: 18 Shuangqing Rd, Beijing 100085, China (Bin Zhao). University of Birmingham, Birmingham B15 2TT, UK (Peng Zhang, Zhiling Guo).School of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UKState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China; Institute of Environment and Health, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, ChinaInstitute of Environmental Sciences (CML), Leiden University, Leiden 2300 RA, the NetherlandsState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaInstitute of High Energy Physics Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China; Institute of Environment and Health, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, ChinaInstitute of High Energy Physics Chinese Academy of Sciences, Beijing 100049, ChinaSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UKSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UKState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China; Institute of Environment and Health, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China; Corresponding authors at: 18 Shuangqing Rd, Beijing 100085, China (Bin Zhao). University of Birmingham, Birmingham B15 2TT, UK (Peng Zhang, Zhiling Guo).Toxicity of ZnO nanoparticles (NPs) are often related to the release of Zn2+ ions due to their dissolution. Studies also suggest that the toxicity of ZnO NPs cannot be solely explained by the release of Zn2+ ions; however, there is a lack of direct evidence of ZnO particulate effects. This study compared the acute toxicity of ZnO NPs and ZnSO4 following intranasal exposure using a combination of metallomics and metabolomics approaches. Significant accumulation of Zn in the liver was only found in the ZnO NP treatment, with 29% of the newly accumulated Zn in the form of ZnO as revealed by X-ray fine structure spectroscopy (XAFS). This is the first direct evidence suggesting the persistence of ZnO NPs in liver upon intranasal exposure. Although both ZnO NPs and ZnSO4 altered the metabolite profiles, with some overlaps and considerable specificity, of both liver and plasma samples, more and distinct metabolites in the liver and opposite effects in the plasma were altered by ZnO NPs compared with ZnSO4, consistent with no accumulation of Zn detected in liver from ZnSO4. Specifically, a large number of antioxidant-related compounds and energetic substrates were exclusively elevated in the liver of ZnO NP-treated animals. These findings provided direct evidence that persistence of ZnO NPs induced particle-specific effects on the antioxidant systems and energy metabolism pathways. Keywords: Zinc oxide nanoparticles, Zinc ions, Metabolite profile, Energy metabolism, oxidative stresshttp://www.sciencedirect.com/science/article/pii/S016041201934142X |