Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris

Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris

The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors. Based on cloned new plasmids pPIC3.5-PreS2-S (8707 bp) and pPIC9-PreS2-S (898...

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Main Authors: Sobirdjan A. Sasmakov, Oybek N. Ashirov, Jaloliddin M. Abdurakhmanov, Shukhrat Sh. Khasanov, Farkhod B. Eshboev, Elvira G. Yusupova, Galina A. Piyakina, Shakhnoz S. Azimova
Format: Article
Language:English
Published: Finlay Ediciones 2021-02-01
Series:VacciMonitor
Subjects:
pichia
hepatitis b virus
recombinant dna
recombinant proteins
Online Access:http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2021000100027&lng=es&nrm=iso&tlng=en
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spelling doaj-90aafd3ea5f74a9586bac90f6a82140c2021-02-16T18:10:36ZengFinlay EdicionesVacciMonitor1025-028X1025-02982021-02-013012732Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastorisSobirdjan A. Sasmakov0https://orcid.org/0000-0003-1863-7117Oybek N. Ashirov1https://orcid.org/0000-0002-0855-6105Jaloliddin M. Abdurakhmanov2https://orcid.org/0000-0002-3954-3251Shukhrat Sh. Khasanov3https://orcid.org/0000-0003-4773-7117Farkhod B. Eshboev4https://orcid.org/0000-0003-3054-6111Elvira G. Yusupova5https://orcid.org/0000-0003-4100-0632Galina A. Piyakina6https://orcid.org/0000-0001-5370-9900Shakhnoz S. Azimova7https://orcid.org/0000-0003-1803-7728Acad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, M. Ulugbek Str. 77, 100170, Tashkent, UzbekistanAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, M. Ulugbek Str. 77, 100170, Tashkent, UzbekistanAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, M. Ulugbek Str. 77, 100170, Tashkent, UzbekistanAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, M. Ulugbek Str. 77, 100170, Tashkent, UzbekistanAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, M. Ulugbek Str. 77, 100170, Tashkent, UzbekistanAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, M. Ulugbek Str. 77, 100170, Tashkent, UzbekistanAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, M. Ulugbek Str. 77, 100170, Tashkent, UzbekistanAcad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances, M. Ulugbek Str. 77, 100170, Tashkent, UzbekistanThe aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors. Based on cloned new plasmids pPIC3.5-PreS2-S (8707 bp) and pPIC9-PreS2-S (8980 bp) the recombinant strains of P. pastoris producing the PreS2-S region of surface antigen of HBV were obtained. The PAGE electrophoresis and immunoblotting of obtained recombinant PreS2-S protein confirm the molecular weight (34 kDa) and high specificity to the HBV antibodies.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2021000100027&lng=es&nrm=iso&tlng=enpichiahepatitis b virusrecombinant dnarecombinant proteins
collection DOAJ
language English
format Article
sources DOAJ
author Sobirdjan A. Sasmakov
Oybek N. Ashirov
Jaloliddin M. Abdurakhmanov
Shukhrat Sh. Khasanov
Farkhod B. Eshboev
Elvira G. Yusupova
Galina A. Piyakina
Shakhnoz S. Azimova
spellingShingle Sobirdjan A. Sasmakov
Oybek N. Ashirov
Jaloliddin M. Abdurakhmanov
Shukhrat Sh. Khasanov
Farkhod B. Eshboev
Elvira G. Yusupova
Galina A. Piyakina
Shakhnoz S. Azimova
Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris
VacciMonitor
pichia
hepatitis b virus
recombinant dna
recombinant proteins
author_facet Sobirdjan A. Sasmakov
Oybek N. Ashirov
Jaloliddin M. Abdurakhmanov
Shukhrat Sh. Khasanov
Farkhod B. Eshboev
Elvira G. Yusupova
Galina A. Piyakina
Shakhnoz S. Azimova
author_sort Sobirdjan A. Sasmakov
title Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris
title_short Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris
title_full Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris
title_fullStr Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris
title_full_unstemmed Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris
title_sort expression of recombinant pres2-s protein from the hepatitis b virus surface antigen in pichia pastoris
publisher Finlay Ediciones
series VacciMonitor
issn 1025-028X
1025-0298
publishDate 2021-02-01
description The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors. Based on cloned new plasmids pPIC3.5-PreS2-S (8707 bp) and pPIC9-PreS2-S (8980 bp) the recombinant strains of P. pastoris producing the PreS2-S region of surface antigen of HBV were obtained. The PAGE electrophoresis and immunoblotting of obtained recombinant PreS2-S protein confirm the molecular weight (34 kDa) and high specificity to the HBV antibodies.
topic pichia
hepatitis b virus
recombinant dna
recombinant proteins
url http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2021000100027&lng=es&nrm=iso&tlng=en
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